Genomic comparison of Trypanosoma conorhini and Trypanosoma rangeli to Trypanosoma cruzi strains of high and low virulence
Abstract Background Trypanosoma conorhini and Trypanosoma rangeli, like Trypanosoma cruzi, are kinetoplastid protist parasites of mammals displaying divergent hosts, geographic ranges and lifestyles. Largely nonpathogenic T. rangeli and T. conorhini represent clades that are phylogenetically closely...
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doaj-90b4383edab44c22a96c02c7c23d70a52020-11-25T00:57:18ZengBMCBMC Genomics1471-21642018-10-0119112010.1186/s12864-018-5112-0Genomic comparison of Trypanosoma conorhini and Trypanosoma rangeli to Trypanosoma cruzi strains of high and low virulenceKatie R Bradwell0Vishal N Koparde1Andrey V Matveyev2Myrna G Serrano3João M P Alves4Hardik Parikh5Bernice Huang6Vladimir Lee7Oneida Espinosa-Alvarez8Paola A Ortiz9André G Costa-Martins10Marta M G Teixeira11Gregory A Buck12Center for the Study of Biological Complexity, Virginia Commonwealth UniversityCenter for the Study of Biological Complexity, Virginia Commonwealth UniversityCenter for the Study of Biological Complexity, Virginia Commonwealth UniversityCenter for the Study of Biological Complexity, Virginia Commonwealth UniversityDepartment of Parasitology, ICB, University of São PauloCenter for the Study of Biological Complexity, Virginia Commonwealth UniversityCenter for the Study of Biological Complexity, Virginia Commonwealth UniversityCenter for the Study of Biological Complexity, Virginia Commonwealth UniversityDepartment of Parasitology, ICB, University of São PauloDepartment of Parasitology, ICB, University of São PauloDepartment of Parasitology, ICB, University of São PauloDepartment of Parasitology, ICB, University of São PauloCenter for the Study of Biological Complexity, Virginia Commonwealth UniversityAbstract Background Trypanosoma conorhini and Trypanosoma rangeli, like Trypanosoma cruzi, are kinetoplastid protist parasites of mammals displaying divergent hosts, geographic ranges and lifestyles. Largely nonpathogenic T. rangeli and T. conorhini represent clades that are phylogenetically closely related to the T. cruzi and T. cruzi-like taxa and provide insights into the evolution of pathogenicity in those parasites. T. rangeli, like T. cruzi is endemic in many Latin American countries, whereas T. conorhini is tropicopolitan. T. rangeli and T. conorhini are exclusively extracellular, while T. cruzi has an intracellular stage in the mammalian host. Results Here we provide the first comprehensive sequence analysis of T. rangeli AM80 and T. conorhini 025E, and provide a comparison of their genomes to those of T. cruzi G and T. cruzi CL, respectively members of T. cruzi lineages TcI and TcVI. We report de novo assembled genome sequences of the low-virulent T. cruzi G, T. rangeli AM80, and T. conorhini 025E ranging from ~ 21–25 Mbp, with ~ 10,000 to 13,000 genes, and for the highly virulent and hybrid T. cruzi CL we present a ~ 65 Mbp in-house assembled haplotyped genome with ~ 12,500 genes per haplotype. Single copy orthologs of the two T. cruzi strains exhibited ~ 97% amino acid identity, and ~ 78% identity to proteins of T. rangeli or T. conorhini. Proteins of the latter two organisms exhibited ~ 84% identity. T. cruzi CL exhibited the highest heterozygosity. T. rangeli and T. conorhini displayed greater metabolic capabilities for utilization of complex carbohydrates, and contained fewer retrotransposons and multigene family copies, i.e. trans-sialidases, mucins, DGF-1, and MASP, compared to T. cruzi. Conclusions Our analyses of the T. rangeli and T. conorhini genomes closely reflected their phylogenetic proximity to the T. cruzi clade, and were largely consistent with their divergent life cycles. Our results provide a greater context for understanding the life cycles, host range expansion, immunity evasion, and pathogenesis of these trypanosomatids.http://link.springer.com/article/10.1186/s12864-018-5112-0TrypanosomatidsComparative genomicsGenome sequencing |
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DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Katie R Bradwell Vishal N Koparde Andrey V Matveyev Myrna G Serrano João M P Alves Hardik Parikh Bernice Huang Vladimir Lee Oneida Espinosa-Alvarez Paola A Ortiz André G Costa-Martins Marta M G Teixeira Gregory A Buck |
spellingShingle |
Katie R Bradwell Vishal N Koparde Andrey V Matveyev Myrna G Serrano João M P Alves Hardik Parikh Bernice Huang Vladimir Lee Oneida Espinosa-Alvarez Paola A Ortiz André G Costa-Martins Marta M G Teixeira Gregory A Buck Genomic comparison of Trypanosoma conorhini and Trypanosoma rangeli to Trypanosoma cruzi strains of high and low virulence BMC Genomics Trypanosomatids Comparative genomics Genome sequencing |
author_facet |
Katie R Bradwell Vishal N Koparde Andrey V Matveyev Myrna G Serrano João M P Alves Hardik Parikh Bernice Huang Vladimir Lee Oneida Espinosa-Alvarez Paola A Ortiz André G Costa-Martins Marta M G Teixeira Gregory A Buck |
author_sort |
Katie R Bradwell |
title |
Genomic comparison of Trypanosoma conorhini and Trypanosoma rangeli to Trypanosoma cruzi strains of high and low virulence |
title_short |
Genomic comparison of Trypanosoma conorhini and Trypanosoma rangeli to Trypanosoma cruzi strains of high and low virulence |
title_full |
Genomic comparison of Trypanosoma conorhini and Trypanosoma rangeli to Trypanosoma cruzi strains of high and low virulence |
title_fullStr |
Genomic comparison of Trypanosoma conorhini and Trypanosoma rangeli to Trypanosoma cruzi strains of high and low virulence |
title_full_unstemmed |
Genomic comparison of Trypanosoma conorhini and Trypanosoma rangeli to Trypanosoma cruzi strains of high and low virulence |
title_sort |
genomic comparison of trypanosoma conorhini and trypanosoma rangeli to trypanosoma cruzi strains of high and low virulence |
publisher |
BMC |
series |
BMC Genomics |
issn |
1471-2164 |
publishDate |
2018-10-01 |
description |
Abstract Background Trypanosoma conorhini and Trypanosoma rangeli, like Trypanosoma cruzi, are kinetoplastid protist parasites of mammals displaying divergent hosts, geographic ranges and lifestyles. Largely nonpathogenic T. rangeli and T. conorhini represent clades that are phylogenetically closely related to the T. cruzi and T. cruzi-like taxa and provide insights into the evolution of pathogenicity in those parasites. T. rangeli, like T. cruzi is endemic in many Latin American countries, whereas T. conorhini is tropicopolitan. T. rangeli and T. conorhini are exclusively extracellular, while T. cruzi has an intracellular stage in the mammalian host. Results Here we provide the first comprehensive sequence analysis of T. rangeli AM80 and T. conorhini 025E, and provide a comparison of their genomes to those of T. cruzi G and T. cruzi CL, respectively members of T. cruzi lineages TcI and TcVI. We report de novo assembled genome sequences of the low-virulent T. cruzi G, T. rangeli AM80, and T. conorhini 025E ranging from ~ 21–25 Mbp, with ~ 10,000 to 13,000 genes, and for the highly virulent and hybrid T. cruzi CL we present a ~ 65 Mbp in-house assembled haplotyped genome with ~ 12,500 genes per haplotype. Single copy orthologs of the two T. cruzi strains exhibited ~ 97% amino acid identity, and ~ 78% identity to proteins of T. rangeli or T. conorhini. Proteins of the latter two organisms exhibited ~ 84% identity. T. cruzi CL exhibited the highest heterozygosity. T. rangeli and T. conorhini displayed greater metabolic capabilities for utilization of complex carbohydrates, and contained fewer retrotransposons and multigene family copies, i.e. trans-sialidases, mucins, DGF-1, and MASP, compared to T. cruzi. Conclusions Our analyses of the T. rangeli and T. conorhini genomes closely reflected their phylogenetic proximity to the T. cruzi clade, and were largely consistent with their divergent life cycles. Our results provide a greater context for understanding the life cycles, host range expansion, immunity evasion, and pathogenesis of these trypanosomatids. |
topic |
Trypanosomatids Comparative genomics Genome sequencing |
url |
http://link.springer.com/article/10.1186/s12864-018-5112-0 |
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