Low extracellular magnesium does not impair glucose-stimulated insulin secretion.
There is an increasing amount of clinical evidence that hypomagnesemia (serum Mg2+ levels < 0.7 mmol/l) contributes to type 2 diabetes mellitus pathogenesis. Amongst other hypotheses, it has been suggested that Mg2+ deficiency affects insulin secretion. The aim of this study was, therefore, to in...
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Online Access: | https://doi.org/10.1371/journal.pone.0217925 |
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doaj-90b8f5467ce0413fa566aa8533b529362021-03-04T12:38:30ZengPublic Library of Science (PLoS)PLoS ONE1932-62032019-01-01146e021792510.1371/journal.pone.0217925Low extracellular magnesium does not impair glucose-stimulated insulin secretion.Lisanne M M GommersThomas G HillFrances M AshcroftJeroen H F de BaaijThere is an increasing amount of clinical evidence that hypomagnesemia (serum Mg2+ levels < 0.7 mmol/l) contributes to type 2 diabetes mellitus pathogenesis. Amongst other hypotheses, it has been suggested that Mg2+ deficiency affects insulin secretion. The aim of this study was, therefore, to investigate the acute effects of extracellular Mg2+ on glucose-stimulated insulin secretion in primary mouse islets of Langerhans and the rat insulinoma INS-1 cell line. Here we show that acute lowering of extracellular Mg2+ concentrations from 1.0 mM to 0.5 mM did not affect glucose-stimulated insulin secretion in islets or in insulin-secreting INS-1 cells. The expression of key genes in the insulin secretory pathway (e.g. Gck, Abcc8) was also unchanged in both experimental models. Knockdown of the most abundant Mg2+ channel Trpm7 by siRNAs in INS-1 cells resulted in a 3-fold increase in insulin secretion at stimulatory glucose conditions compared to mock-transfected cells. Our data suggest that insulin secretion is not affected by acute lowering of extracellular Mg2+ concentrations.https://doi.org/10.1371/journal.pone.0217925 |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Lisanne M M Gommers Thomas G Hill Frances M Ashcroft Jeroen H F de Baaij |
spellingShingle |
Lisanne M M Gommers Thomas G Hill Frances M Ashcroft Jeroen H F de Baaij Low extracellular magnesium does not impair glucose-stimulated insulin secretion. PLoS ONE |
author_facet |
Lisanne M M Gommers Thomas G Hill Frances M Ashcroft Jeroen H F de Baaij |
author_sort |
Lisanne M M Gommers |
title |
Low extracellular magnesium does not impair glucose-stimulated insulin secretion. |
title_short |
Low extracellular magnesium does not impair glucose-stimulated insulin secretion. |
title_full |
Low extracellular magnesium does not impair glucose-stimulated insulin secretion. |
title_fullStr |
Low extracellular magnesium does not impair glucose-stimulated insulin secretion. |
title_full_unstemmed |
Low extracellular magnesium does not impair glucose-stimulated insulin secretion. |
title_sort |
low extracellular magnesium does not impair glucose-stimulated insulin secretion. |
publisher |
Public Library of Science (PLoS) |
series |
PLoS ONE |
issn |
1932-6203 |
publishDate |
2019-01-01 |
description |
There is an increasing amount of clinical evidence that hypomagnesemia (serum Mg2+ levels < 0.7 mmol/l) contributes to type 2 diabetes mellitus pathogenesis. Amongst other hypotheses, it has been suggested that Mg2+ deficiency affects insulin secretion. The aim of this study was, therefore, to investigate the acute effects of extracellular Mg2+ on glucose-stimulated insulin secretion in primary mouse islets of Langerhans and the rat insulinoma INS-1 cell line. Here we show that acute lowering of extracellular Mg2+ concentrations from 1.0 mM to 0.5 mM did not affect glucose-stimulated insulin secretion in islets or in insulin-secreting INS-1 cells. The expression of key genes in the insulin secretory pathway (e.g. Gck, Abcc8) was also unchanged in both experimental models. Knockdown of the most abundant Mg2+ channel Trpm7 by siRNAs in INS-1 cells resulted in a 3-fold increase in insulin secretion at stimulatory glucose conditions compared to mock-transfected cells. Our data suggest that insulin secretion is not affected by acute lowering of extracellular Mg2+ concentrations. |
url |
https://doi.org/10.1371/journal.pone.0217925 |
work_keys_str_mv |
AT lisannemmgommers lowextracellularmagnesiumdoesnotimpairglucosestimulatedinsulinsecretion AT thomasghill lowextracellularmagnesiumdoesnotimpairglucosestimulatedinsulinsecretion AT francesmashcroft lowextracellularmagnesiumdoesnotimpairglucosestimulatedinsulinsecretion AT jeroenhfdebaaij lowextracellularmagnesiumdoesnotimpairglucosestimulatedinsulinsecretion |
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1714802043822014464 |