Sequencing, Expression, and Functional Analyses of Four Genes Related to Fatty Acid Biosynthesis During the Diapause Process in the Female Ladybird, Coccinella septempunctata L.
The ladybird Coccinella septempunctata L., a predatory insect, serves as an excellent biological control agent against common agricultural pests. It undergoes a diapause phenomenon, during which a large amount of fat accumulates in the abdomen. A comprehensive analysis of this lipid accumulation can...
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doaj-91025f84c1d1424fab655748e3c8ad6f2021-08-19T10:38:00ZengFrontiers Media S.A.Frontiers in Physiology1664-042X2021-08-011210.3389/fphys.2021.706032706032Sequencing, Expression, and Functional Analyses of Four Genes Related to Fatty Acid Biosynthesis During the Diapause Process in the Female Ladybird, Coccinella septempunctata L.Mei Xiang0Mei Xiang1Hong-Zhi Zhang2Xiao-Yu Jing3Xiao-Yu Jing4Meng-Qing Wang5Jian-Jun Mao6Yu-Yan Li7Lian-Sheng Zang8Li-Sheng Zhang9Institute of Plant Protection, Chinese Academy of Agricultural Sciences, Beijing, ChinaInstitute of Biological Control, Jilin Agricultural University, Changchun, ChinaDepartment of Entomology and BIO5 Institute, University of Arizona, Tucson, AZ, United StatesInstitute of Plant Protection, Chinese Academy of Agricultural Sciences, Beijing, ChinaInstitute of Biological Control, Jilin Agricultural University, Changchun, ChinaInstitute of Plant Protection, Chinese Academy of Agricultural Sciences, Beijing, ChinaInstitute of Plant Protection, Chinese Academy of Agricultural Sciences, Beijing, ChinaInstitute of Plant Protection, Chinese Academy of Agricultural Sciences, Beijing, ChinaInstitute of Biological Control, Jilin Agricultural University, Changchun, ChinaInstitute of Plant Protection, Chinese Academy of Agricultural Sciences, Beijing, ChinaThe ladybird Coccinella septempunctata L., a predatory insect, serves as an excellent biological control agent against common agricultural pests. It undergoes a diapause phenomenon, during which a large amount of fat accumulates in the abdomen. A comprehensive analysis of this lipid accumulation can reveal the molecular mechanisms underlying diapause regulation, which can be exploited to improve the shipping and transport of the insect for agricultural applications. In this study, we compared the transcriptome of C. septempunctata during non-diapause, diapause, and post-diapause and screened four key genes related to lipid metabolism. The cDNA of these four relevant enzymes, acetyl-CoA carboxylase (ACC), long-chain fatty acid-CoA ligase (ACSL), elongase of very-long-chain fatty acids (ELO), and very-long-chain 3-oxoacyl-CoA reductase (KAR), were cloned using reverse transcription-polymerase chain reaction and rapid amplification of cDNA ends. Their expression profiles were analyzed during the preparation and maintenance phases of diapause and the post-diapause phase. The functions of these four key enzymes in diapause were further verified using RNA interference. All four genes were most closely related to the homeotic gene from Tribolium castaneum. The expression profiles of these four genes were significantly affected under diapause-inducing conditions; their expression level was the highest in the diapause preparation phase, and it gradually decreased with the diapause induction time. RNA interference showed that the target genes play important roles in fat storage during early diapause, and the decrease in their expression leads to a decrease in lipid content in C. septempunctata. These results indicate an important role of ACC, ACSL, ELO, and KAR in lipid accumulation. Our findings could help elucidate the production and accumulation of lipids by insects during the preparation for diapause and improve biological control.https://www.frontiersin.org/articles/10.3389/fphys.2021.706032/fullCoccinella septempunctatadiapausecloningexpressionRNAi |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Mei Xiang Mei Xiang Hong-Zhi Zhang Xiao-Yu Jing Xiao-Yu Jing Meng-Qing Wang Jian-Jun Mao Yu-Yan Li Lian-Sheng Zang Li-Sheng Zhang |
spellingShingle |
Mei Xiang Mei Xiang Hong-Zhi Zhang Xiao-Yu Jing Xiao-Yu Jing Meng-Qing Wang Jian-Jun Mao Yu-Yan Li Lian-Sheng Zang Li-Sheng Zhang Sequencing, Expression, and Functional Analyses of Four Genes Related to Fatty Acid Biosynthesis During the Diapause Process in the Female Ladybird, Coccinella septempunctata L. Frontiers in Physiology Coccinella septempunctata diapause cloning expression RNAi |
author_facet |
Mei Xiang Mei Xiang Hong-Zhi Zhang Xiao-Yu Jing Xiao-Yu Jing Meng-Qing Wang Jian-Jun Mao Yu-Yan Li Lian-Sheng Zang Li-Sheng Zhang |
author_sort |
Mei Xiang |
title |
Sequencing, Expression, and Functional Analyses of Four Genes Related to Fatty Acid Biosynthesis During the Diapause Process in the Female Ladybird, Coccinella septempunctata L. |
title_short |
Sequencing, Expression, and Functional Analyses of Four Genes Related to Fatty Acid Biosynthesis During the Diapause Process in the Female Ladybird, Coccinella septempunctata L. |
title_full |
Sequencing, Expression, and Functional Analyses of Four Genes Related to Fatty Acid Biosynthesis During the Diapause Process in the Female Ladybird, Coccinella septempunctata L. |
title_fullStr |
Sequencing, Expression, and Functional Analyses of Four Genes Related to Fatty Acid Biosynthesis During the Diapause Process in the Female Ladybird, Coccinella septempunctata L. |
title_full_unstemmed |
Sequencing, Expression, and Functional Analyses of Four Genes Related to Fatty Acid Biosynthesis During the Diapause Process in the Female Ladybird, Coccinella septempunctata L. |
title_sort |
sequencing, expression, and functional analyses of four genes related to fatty acid biosynthesis during the diapause process in the female ladybird, coccinella septempunctata l. |
publisher |
Frontiers Media S.A. |
series |
Frontiers in Physiology |
issn |
1664-042X |
publishDate |
2021-08-01 |
description |
The ladybird Coccinella septempunctata L., a predatory insect, serves as an excellent biological control agent against common agricultural pests. It undergoes a diapause phenomenon, during which a large amount of fat accumulates in the abdomen. A comprehensive analysis of this lipid accumulation can reveal the molecular mechanisms underlying diapause regulation, which can be exploited to improve the shipping and transport of the insect for agricultural applications. In this study, we compared the transcriptome of C. septempunctata during non-diapause, diapause, and post-diapause and screened four key genes related to lipid metabolism. The cDNA of these four relevant enzymes, acetyl-CoA carboxylase (ACC), long-chain fatty acid-CoA ligase (ACSL), elongase of very-long-chain fatty acids (ELO), and very-long-chain 3-oxoacyl-CoA reductase (KAR), were cloned using reverse transcription-polymerase chain reaction and rapid amplification of cDNA ends. Their expression profiles were analyzed during the preparation and maintenance phases of diapause and the post-diapause phase. The functions of these four key enzymes in diapause were further verified using RNA interference. All four genes were most closely related to the homeotic gene from Tribolium castaneum. The expression profiles of these four genes were significantly affected under diapause-inducing conditions; their expression level was the highest in the diapause preparation phase, and it gradually decreased with the diapause induction time. RNA interference showed that the target genes play important roles in fat storage during early diapause, and the decrease in their expression leads to a decrease in lipid content in C. septempunctata. These results indicate an important role of ACC, ACSL, ELO, and KAR in lipid accumulation. Our findings could help elucidate the production and accumulation of lipids by insects during the preparation for diapause and improve biological control. |
topic |
Coccinella septempunctata diapause cloning expression RNAi |
url |
https://www.frontiersin.org/articles/10.3389/fphys.2021.706032/full |
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