Nanopriming technology for enhancing germination and starch metabolism of aged rice seeds using phytosynthesized silver nanoparticles
Abstract Application of nanomaterials for agriculture is relatively new as compared to their use in biomedical and industrial sectors. In order to promote sustainable nanoagriculture, biocompatible silver nanoparticles (AgNPs) have been synthesized through green route using kaffir lime leaf extract...
Main Authors: | , , , |
---|---|
Format: | Article |
Language: | English |
Published: |
Nature Publishing Group
2017-08-01
|
Series: | Scientific Reports |
Online Access: | https://doi.org/10.1038/s41598-017-08669-5 |
id |
doaj-91061e9279da4c00982fc6f909567d02 |
---|---|
record_format |
Article |
spelling |
doaj-91061e9279da4c00982fc6f909567d022020-12-08T01:00:16ZengNature Publishing GroupScientific Reports2045-23222017-08-017112110.1038/s41598-017-08669-5Nanopriming technology for enhancing germination and starch metabolism of aged rice seeds using phytosynthesized silver nanoparticlesWuttipong Mahakham0Ajit K. Sarmah1Santi Maensiri2Piyada Theerakulpisut3Department of Civil and Environmental Engineering, Faculty of Engineering, The University of Auckland, Private Bag 92019Department of Civil and Environmental Engineering, Faculty of Engineering, The University of Auckland, Private Bag 92019School of Physics, Institute of Science, Suranaree University of TechnologySalt-tolerant Rice Research Group, Department of Biology, Faculty of Science, Khon Kaen UniversityAbstract Application of nanomaterials for agriculture is relatively new as compared to their use in biomedical and industrial sectors. In order to promote sustainable nanoagriculture, biocompatible silver nanoparticles (AgNPs) have been synthesized through green route using kaffir lime leaf extract for use as nanopriming agent for enhancing seed germination of rice aged seeds. Results of various characterization techniques showed the successful formation of AgNPs which were capped with phytochemicals present in the plant extract. Rice aged seeds primed with phytosynthesized AgNPs at 5 and 10 ppm significantly improved germination performance and seedling vigor compared to unprimed control, AgNO3 priming, and conventional hydropriming. Nanopriming could enhance α-amylase activity, resulting in higher soluble sugar content for supporting seedlings growth. Furthermore, nanopriming stimulated the up-regulation of aquaporin genes in germinating seeds. Meanwhile, more ROS production was observed in germinating seeds of nanopriming treatment compared to unprimed control and other priming treatments, suggesting that both ROS and aquaporins play important roles in enhancing seed germination. Different mechanisms underlying nanopriming-induced seed germination were proposed, including creation of nanopores for enhanced water uptake, rebooting ROS/antioxidant systems in seeds, generation of hydroxyl radicals for cell wall loosening, and nanocatalyst for fastening starch hydrolysis.https://doi.org/10.1038/s41598-017-08669-5 |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Wuttipong Mahakham Ajit K. Sarmah Santi Maensiri Piyada Theerakulpisut |
spellingShingle |
Wuttipong Mahakham Ajit K. Sarmah Santi Maensiri Piyada Theerakulpisut Nanopriming technology for enhancing germination and starch metabolism of aged rice seeds using phytosynthesized silver nanoparticles Scientific Reports |
author_facet |
Wuttipong Mahakham Ajit K. Sarmah Santi Maensiri Piyada Theerakulpisut |
author_sort |
Wuttipong Mahakham |
title |
Nanopriming technology for enhancing germination and starch metabolism of aged rice seeds using phytosynthesized silver nanoparticles |
title_short |
Nanopriming technology for enhancing germination and starch metabolism of aged rice seeds using phytosynthesized silver nanoparticles |
title_full |
Nanopriming technology for enhancing germination and starch metabolism of aged rice seeds using phytosynthesized silver nanoparticles |
title_fullStr |
Nanopriming technology for enhancing germination and starch metabolism of aged rice seeds using phytosynthesized silver nanoparticles |
title_full_unstemmed |
Nanopriming technology for enhancing germination and starch metabolism of aged rice seeds using phytosynthesized silver nanoparticles |
title_sort |
nanopriming technology for enhancing germination and starch metabolism of aged rice seeds using phytosynthesized silver nanoparticles |
publisher |
Nature Publishing Group |
series |
Scientific Reports |
issn |
2045-2322 |
publishDate |
2017-08-01 |
description |
Abstract Application of nanomaterials for agriculture is relatively new as compared to their use in biomedical and industrial sectors. In order to promote sustainable nanoagriculture, biocompatible silver nanoparticles (AgNPs) have been synthesized through green route using kaffir lime leaf extract for use as nanopriming agent for enhancing seed germination of rice aged seeds. Results of various characterization techniques showed the successful formation of AgNPs which were capped with phytochemicals present in the plant extract. Rice aged seeds primed with phytosynthesized AgNPs at 5 and 10 ppm significantly improved germination performance and seedling vigor compared to unprimed control, AgNO3 priming, and conventional hydropriming. Nanopriming could enhance α-amylase activity, resulting in higher soluble sugar content for supporting seedlings growth. Furthermore, nanopriming stimulated the up-regulation of aquaporin genes in germinating seeds. Meanwhile, more ROS production was observed in germinating seeds of nanopriming treatment compared to unprimed control and other priming treatments, suggesting that both ROS and aquaporins play important roles in enhancing seed germination. Different mechanisms underlying nanopriming-induced seed germination were proposed, including creation of nanopores for enhanced water uptake, rebooting ROS/antioxidant systems in seeds, generation of hydroxyl radicals for cell wall loosening, and nanocatalyst for fastening starch hydrolysis. |
url |
https://doi.org/10.1038/s41598-017-08669-5 |
work_keys_str_mv |
AT wuttipongmahakham nanoprimingtechnologyforenhancinggerminationandstarchmetabolismofagedriceseedsusingphytosynthesizedsilvernanoparticles AT ajitksarmah nanoprimingtechnologyforenhancinggerminationandstarchmetabolismofagedriceseedsusingphytosynthesizedsilvernanoparticles AT santimaensiri nanoprimingtechnologyforenhancinggerminationandstarchmetabolismofagedriceseedsusingphytosynthesizedsilvernanoparticles AT piyadatheerakulpisut nanoprimingtechnologyforenhancinggerminationandstarchmetabolismofagedriceseedsusingphytosynthesizedsilvernanoparticles |
_version_ |
1724395408531128320 |