Dataset of plasmid DNA extraction using different magnetic nanoparticles (MNPs)
In this dataset we integrated figures related to bacterial transformation using pBI121 plasmid and complementary analysis for magnetic nanoparticles (MNPs) characterizations. The structural map of pBI121 plasmid was drawn by Vector NTI software using the complete sequence of binary vector pBI121. Es...
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doaj-911225525cc1413984293a09ab33b1c02020-11-25T01:51:11ZengElsevierData in Brief2352-34092016-12-019C80781110.1016/j.dib.2016.10.013Dataset of plasmid DNA extraction using different magnetic nanoparticles (MNPs)H. Rahnama0A. Sattarzadeh1F. Kazemi2N. Ahmadi3F. Sanjarian4Z. Zand5Agricultural Biotechnology Research Institute of Iran (ABRII), Agricultural Research, Education and Extension Organization (AREEO), Karaj, IranAgricultural Biotechnology Research Institute of Iran (ABRII), Agricultural Research, Education and Extension Organization (AREEO), Karaj, IranInstitute for Advanced Studies in Basic Sciences (IASBS), Zanjan, IranAgricultural Biotechnology Research Institute of Iran (ABRII), Agricultural Research, Education and Extension Organization (AREEO), Karaj, IranNational Institute of Genetic Engineering and Biotechnology (NIGEB), Tehran, IranInstitute for Advanced Studies in Basic Sciences (IASBS), Zanjan, IranIn this dataset we integrated figures related to bacterial transformation using pBI121 plasmid and complementary analysis for magnetic nanoparticles (MNPs) characterizations. The structural map of pBI121 plasmid was drawn by Vector NTI software using the complete sequence of binary vector pBI121. Escherichia coli bacteria transformed using pBI121 plasmid and were grown on the selection media containing kanamycin. MNPs were characterized by energy dispersive spectroscopy (EDS) and transmission electron microscopy (TEM). Finally, the overall efficiency of different MNPs (Fe3O4, Fe3O4/SiO2, Fe3O4/SiO2/TiO2) in plasmid DNA isolation was compared using gel electrophoresis analysis. The data supplied in this article supports the accompanying publication “Comparative study of three magnetic nano-particles (FeSO4, FeSO4/SiO2, FeSO4/SiO2/TiO2) in plasmid DNA extraction” (H. Rahnama, A. Sattarzadeh, F. Kazemi, N. Ahmadi, F. Sanjarian, Z. Zand, 2016) [1].http://www.sciencedirect.com/science/article/pii/S2352340916306400pBI121BacteriaMagnetic nanoparticlesPlasmidTEMEDS |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
H. Rahnama A. Sattarzadeh F. Kazemi N. Ahmadi F. Sanjarian Z. Zand |
spellingShingle |
H. Rahnama A. Sattarzadeh F. Kazemi N. Ahmadi F. Sanjarian Z. Zand Dataset of plasmid DNA extraction using different magnetic nanoparticles (MNPs) Data in Brief pBI121 Bacteria Magnetic nanoparticles Plasmid TEM EDS |
author_facet |
H. Rahnama A. Sattarzadeh F. Kazemi N. Ahmadi F. Sanjarian Z. Zand |
author_sort |
H. Rahnama |
title |
Dataset of plasmid DNA extraction using different magnetic nanoparticles (MNPs) |
title_short |
Dataset of plasmid DNA extraction using different magnetic nanoparticles (MNPs) |
title_full |
Dataset of plasmid DNA extraction using different magnetic nanoparticles (MNPs) |
title_fullStr |
Dataset of plasmid DNA extraction using different magnetic nanoparticles (MNPs) |
title_full_unstemmed |
Dataset of plasmid DNA extraction using different magnetic nanoparticles (MNPs) |
title_sort |
dataset of plasmid dna extraction using different magnetic nanoparticles (mnps) |
publisher |
Elsevier |
series |
Data in Brief |
issn |
2352-3409 |
publishDate |
2016-12-01 |
description |
In this dataset we integrated figures related to bacterial transformation using pBI121 plasmid and complementary analysis for magnetic nanoparticles (MNPs) characterizations. The structural map of pBI121 plasmid was drawn by Vector NTI software using the complete sequence of binary vector pBI121. Escherichia coli bacteria transformed using pBI121 plasmid and were grown on the selection media containing kanamycin.
MNPs were characterized by energy dispersive spectroscopy (EDS) and transmission electron microscopy (TEM). Finally, the overall efficiency of different MNPs (Fe3O4, Fe3O4/SiO2, Fe3O4/SiO2/TiO2) in plasmid DNA isolation was compared using gel electrophoresis analysis. The data supplied in this article supports the accompanying publication “Comparative study of three magnetic nano-particles (FeSO4, FeSO4/SiO2, FeSO4/SiO2/TiO2) in plasmid DNA extraction” (H. Rahnama, A. Sattarzadeh, F. Kazemi, N. Ahmadi, F. Sanjarian, Z. Zand, 2016) [1]. |
topic |
pBI121 Bacteria Magnetic nanoparticles Plasmid TEM EDS |
url |
http://www.sciencedirect.com/science/article/pii/S2352340916306400 |
work_keys_str_mv |
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