Dataset of plasmid DNA extraction using different magnetic nanoparticles (MNPs)

Dataset of plasmid DNA extraction using different magnetic nanoparticles (MNPs)

In this dataset we integrated figures related to bacterial transformation using pBI121 plasmid and complementary analysis for magnetic nanoparticles (MNPs) characterizations. The structural map of pBI121 plasmid was drawn by Vector NTI software using the complete sequence of binary vector pBI121. Es...

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Bibliographic Details
Main Authors: H. Rahnama, A. Sattarzadeh, F. Kazemi, N. Ahmadi, F. Sanjarian, Z. Zand
Format: Article
Language:English
Published: Elsevier 2016-12-01
Series:Data in Brief
Subjects:
pBI121
Bacteria
Magnetic nanoparticles
Plasmid
TEM
EDS
Online Access:http://www.sciencedirect.com/science/article/pii/S2352340916306400
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spelling doaj-911225525cc1413984293a09ab33b1c02020-11-25T01:51:11ZengElsevierData in Brief2352-34092016-12-019C80781110.1016/j.dib.2016.10.013Dataset of plasmid DNA extraction using different magnetic nanoparticles (MNPs)H. Rahnama0A. Sattarzadeh1F. Kazemi2N. Ahmadi3F. Sanjarian4Z. Zand5Agricultural Biotechnology Research Institute of Iran (ABRII), Agricultural Research, Education and Extension Organization (AREEO), Karaj, IranAgricultural Biotechnology Research Institute of Iran (ABRII), Agricultural Research, Education and Extension Organization (AREEO), Karaj, IranInstitute for Advanced Studies in Basic Sciences (IASBS), Zanjan, IranAgricultural Biotechnology Research Institute of Iran (ABRII), Agricultural Research, Education and Extension Organization (AREEO), Karaj, IranNational Institute of Genetic Engineering and Biotechnology (NIGEB), Tehran, IranInstitute for Advanced Studies in Basic Sciences (IASBS), Zanjan, IranIn this dataset we integrated figures related to bacterial transformation using pBI121 plasmid and complementary analysis for magnetic nanoparticles (MNPs) characterizations. The structural map of pBI121 plasmid was drawn by Vector NTI software using the complete sequence of binary vector pBI121. Escherichia coli bacteria transformed using pBI121 plasmid and were grown on the selection media containing kanamycin. MNPs were characterized by energy dispersive spectroscopy (EDS) and transmission electron microscopy (TEM). Finally, the overall efficiency of different MNPs (Fe3O4, Fe3O4/SiO2, Fe3O4/SiO2/TiO2) in plasmid DNA isolation was compared using gel electrophoresis analysis. The data supplied in this article supports the accompanying publication “Comparative study of three magnetic nano-particles (FeSO4, FeSO4/SiO2, FeSO4/SiO2/TiO2) in plasmid DNA extraction” (H. Rahnama, A. Sattarzadeh, F. Kazemi, N. Ahmadi, F. Sanjarian, Z. Zand, 2016) [1].http://www.sciencedirect.com/science/article/pii/S2352340916306400pBI121BacteriaMagnetic nanoparticlesPlasmidTEMEDS
collection DOAJ
language English
format Article
sources DOAJ
author H. Rahnama
A. Sattarzadeh
F. Kazemi
N. Ahmadi
F. Sanjarian
Z. Zand
spellingShingle H. Rahnama
A. Sattarzadeh
F. Kazemi
N. Ahmadi
F. Sanjarian
Z. Zand
Dataset of plasmid DNA extraction using different magnetic nanoparticles (MNPs)
Data in Brief
pBI121
Bacteria
Magnetic nanoparticles
Plasmid
TEM
EDS
author_facet H. Rahnama
A. Sattarzadeh
F. Kazemi
N. Ahmadi
F. Sanjarian
Z. Zand
author_sort H. Rahnama
title Dataset of plasmid DNA extraction using different magnetic nanoparticles (MNPs)
title_short Dataset of plasmid DNA extraction using different magnetic nanoparticles (MNPs)
title_full Dataset of plasmid DNA extraction using different magnetic nanoparticles (MNPs)
title_fullStr Dataset of plasmid DNA extraction using different magnetic nanoparticles (MNPs)
title_full_unstemmed Dataset of plasmid DNA extraction using different magnetic nanoparticles (MNPs)
title_sort dataset of plasmid dna extraction using different magnetic nanoparticles (mnps)
publisher Elsevier
series Data in Brief
issn 2352-3409
publishDate 2016-12-01
description In this dataset we integrated figures related to bacterial transformation using pBI121 plasmid and complementary analysis for magnetic nanoparticles (MNPs) characterizations. The structural map of pBI121 plasmid was drawn by Vector NTI software using the complete sequence of binary vector pBI121. Escherichia coli bacteria transformed using pBI121 plasmid and were grown on the selection media containing kanamycin. MNPs were characterized by energy dispersive spectroscopy (EDS) and transmission electron microscopy (TEM). Finally, the overall efficiency of different MNPs (Fe3O4, Fe3O4/SiO2, Fe3O4/SiO2/TiO2) in plasmid DNA isolation was compared using gel electrophoresis analysis. The data supplied in this article supports the accompanying publication “Comparative study of three magnetic nano-particles (FeSO4, FeSO4/SiO2, FeSO4/SiO2/TiO2) in plasmid DNA extraction” (H. Rahnama, A. Sattarzadeh, F. Kazemi, N. Ahmadi, F. Sanjarian, Z. Zand, 2016) [1].
topic pBI121
Bacteria
Magnetic nanoparticles
Plasmid
TEM
EDS
url http://www.sciencedirect.com/science/article/pii/S2352340916306400
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