Genetic variation on internal protein matric (M1) and non structural protein (NS1) of Indonesian avian influenza virus H5N1 subtype
The mutation and genetic variation of avian influenza virus ussually associated with Hemmaglutinin (HA) and Neuraminidase (NA). The HA and NA protein are surface glycoproteins which have role for receptor binding site of the virus, determine virus subtype and genetic variation occured in those prote...
Main Author: | |
---|---|
Format: | Article |
Language: | English |
Published: |
Pusat Penelitian dan Pengembangan Peternakan
2011-03-01
|
Series: | Jurnal Ilmu Ternak dan Veteriner |
Subjects: | |
Online Access: | http://medpub.litbang.pertanian.go.id/index.php/jitv/article/view/636/645 |
id |
doaj-911b77a38adb4e7784ee216922c5aa01 |
---|---|
record_format |
Article |
spelling |
doaj-911b77a38adb4e7784ee216922c5aa012020-11-24T22:46:08ZengPusat Penelitian dan Pengembangan PeternakanJurnal Ilmu Ternak dan Veteriner0853-73802252-696X2011-03-011617181Genetic variation on internal protein matric (M1) and non structural protein (NS1) of Indonesian avian influenza virus H5N1 subtypeN.L.P. Indi Dharmayanti0—The mutation and genetic variation of avian influenza virus ussually associated with Hemmaglutinin (HA) and Neuraminidase (NA). The HA and NA protein are surface glycoproteins which have role for receptor binding site of the virus, determine virus subtype and genetic variation occured in those proteins. On the other site, the virus have the internal protein that posses function for virus replication. This study analyzed the mutation on the the internal protein virus especially the Matrix (M1) and non structural (NS1) protein and its three dimensioanal structure of proteins. The methods used in this study were virus propagation, (Reverse Transcriptase Polymerase Chain Reaction) RT-PCR sequencing of M1 and NS1 and using DS Modeller dan DS Standalone from Discovery Studio for Modeling and Simulation to predict the three dimentional structure of the proteins. The result of this study showed that Indonesian AI H5N1 subtype had genetic variation internal protein and have no change on conserved of condition such as putative zinc finger and nuclear localization signal (NLS). Genetic variation that occured on PDZ motif of NS1 especially have human origin motif that might be correlated with virus adaptation on human.http://medpub.litbang.pertanian.go.id/index.php/jitv/article/view/636/645Avian InfluenzaMatrix (M1)Non Structural (NS1)Genetic Variation |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
N.L.P. Indi Dharmayanti |
spellingShingle |
N.L.P. Indi Dharmayanti Genetic variation on internal protein matric (M1) and non structural protein (NS1) of Indonesian avian influenza virus H5N1 subtype Jurnal Ilmu Ternak dan Veteriner Avian Influenza Matrix (M1) Non Structural (NS1) Genetic Variation |
author_facet |
N.L.P. Indi Dharmayanti |
author_sort |
N.L.P. Indi Dharmayanti |
title |
Genetic variation on internal protein matric (M1) and non structural protein (NS1) of Indonesian avian influenza virus H5N1 subtype |
title_short |
Genetic variation on internal protein matric (M1) and non structural protein (NS1) of Indonesian avian influenza virus H5N1 subtype |
title_full |
Genetic variation on internal protein matric (M1) and non structural protein (NS1) of Indonesian avian influenza virus H5N1 subtype |
title_fullStr |
Genetic variation on internal protein matric (M1) and non structural protein (NS1) of Indonesian avian influenza virus H5N1 subtype |
title_full_unstemmed |
Genetic variation on internal protein matric (M1) and non structural protein (NS1) of Indonesian avian influenza virus H5N1 subtype |
title_sort |
genetic variation on internal protein matric (m1) and non structural protein (ns1) of indonesian avian influenza virus h5n1 subtype |
publisher |
Pusat Penelitian dan Pengembangan Peternakan |
series |
Jurnal Ilmu Ternak dan Veteriner |
issn |
0853-7380 2252-696X |
publishDate |
2011-03-01 |
description |
The mutation and genetic variation of avian influenza virus ussually associated with Hemmaglutinin (HA) and Neuraminidase (NA). The HA and NA protein are surface glycoproteins which have role for receptor binding site of the virus, determine virus subtype and genetic variation occured in those proteins. On the other site, the virus have the internal protein that posses function for virus replication. This study analyzed the mutation on the the internal protein virus especially the Matrix (M1) and non structural (NS1) protein and its three dimensioanal structure of proteins. The methods used in this study were virus propagation, (Reverse Transcriptase Polymerase Chain Reaction) RT-PCR sequencing of M1 and NS1 and using DS Modeller dan DS Standalone from Discovery Studio for Modeling and Simulation to predict the three dimentional structure of the proteins. The result of this study showed that Indonesian AI H5N1 subtype had genetic variation internal protein and have no change on conserved of condition such as putative zinc finger and nuclear localization signal (NLS). Genetic variation that occured on PDZ motif of NS1 especially have human origin motif that might be correlated with virus adaptation on human. |
topic |
Avian Influenza Matrix (M1) Non Structural (NS1) Genetic Variation |
url |
http://medpub.litbang.pertanian.go.id/index.php/jitv/article/view/636/645 |
work_keys_str_mv |
AT nlpindidharmayanti geneticvariationoninternalproteinmatricm1andnonstructuralproteinns1ofindonesianavianinfluenzavirush5n1subtype |
_version_ |
1725686147978362880 |