Stable Transformation and Expression of GhEXPA8 Fibre Expansin Gene to Improve Fibre Length and Micronaire Value in Cotton
Cotton fibre is multigenic trait controlled by number of genes. Previous studies suggest that one of these genes may be responsible for switching cotton fibre growth on and off to influence the fibre quality produced from a cotton seed. In the present study, the Gossypium hirsutum GhEXPA8 fibre expa...
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doaj-9128d4d72815474299ce255258659d2d2020-11-24T21:24:54ZengFrontiers Media S.A.Frontiers in Plant Science1664-462X2015-10-01610.3389/fpls.2015.00838149710Stable Transformation and Expression of GhEXPA8 Fibre Expansin Gene to Improve Fibre Length and Micronaire Value in CottonKamran Shehzad Bajwa0Ahmad Ali Shahid1Abdul Qayyum Rao2Aftab eBashir3Asia eAftab4Tayyab eHusnain5University of the PunjabUniversity of the PunjabUniversity of the PunjabNuclear Institute of Biotechnology and Genetic Engineering (NIBGE)Nuclear Institute of Biotechnology and Genetic Engineering (NIBGE)University of the PunjabCotton fibre is multigenic trait controlled by number of genes. Previous studies suggest that one of these genes may be responsible for switching cotton fibre growth on and off to influence the fibre quality produced from a cotton seed. In the present study, the Gossypium hirsutum GhEXPA8 fibre expansin gene cloned into a pGA482 plant expression vector with a 2X 35S promoter and a CaMV terminator was introduced into local cotton variety NIAB 846 by using an Agrobacterium-mediated gene transformation. The neomycin phosphotransferase (NPTII) gene was used as a selection marker for screening of putative transgenic cotton plants. Integration and expression of the fibre expansin gene in cotton plants was confirmed with molecular techniques including Southern blot analyses, real-time PCR and cellulose assays. The data collected from three years of field performance of the transgenic cotton plants expressing GhEXPA8 showed that significant improvement has been made in fibre lengths and micronaire values as compared to control Gossypium hirsutum variety NIAB 846 cotton plants. Field data of fibre and morphological characteristics were also analyzed through statistical techniques. The results of this study support improvement of cotton fibre through genetic modification.http://journal.frontiersin.org/Journal/10.3389/fpls.2015.00838/fullAgrobacterium tumefaciensCotton FiberTransformation, GeneticMicronaire valueGhEXPA8 gene |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Kamran Shehzad Bajwa Ahmad Ali Shahid Abdul Qayyum Rao Aftab eBashir Asia eAftab Tayyab eHusnain |
spellingShingle |
Kamran Shehzad Bajwa Ahmad Ali Shahid Abdul Qayyum Rao Aftab eBashir Asia eAftab Tayyab eHusnain Stable Transformation and Expression of GhEXPA8 Fibre Expansin Gene to Improve Fibre Length and Micronaire Value in Cotton Frontiers in Plant Science Agrobacterium tumefaciens Cotton Fiber Transformation, Genetic Micronaire value GhEXPA8 gene |
author_facet |
Kamran Shehzad Bajwa Ahmad Ali Shahid Abdul Qayyum Rao Aftab eBashir Asia eAftab Tayyab eHusnain |
author_sort |
Kamran Shehzad Bajwa |
title |
Stable Transformation and Expression of GhEXPA8 Fibre Expansin Gene to Improve Fibre Length and Micronaire Value in Cotton |
title_short |
Stable Transformation and Expression of GhEXPA8 Fibre Expansin Gene to Improve Fibre Length and Micronaire Value in Cotton |
title_full |
Stable Transformation and Expression of GhEXPA8 Fibre Expansin Gene to Improve Fibre Length and Micronaire Value in Cotton |
title_fullStr |
Stable Transformation and Expression of GhEXPA8 Fibre Expansin Gene to Improve Fibre Length and Micronaire Value in Cotton |
title_full_unstemmed |
Stable Transformation and Expression of GhEXPA8 Fibre Expansin Gene to Improve Fibre Length and Micronaire Value in Cotton |
title_sort |
stable transformation and expression of ghexpa8 fibre expansin gene to improve fibre length and micronaire value in cotton |
publisher |
Frontiers Media S.A. |
series |
Frontiers in Plant Science |
issn |
1664-462X |
publishDate |
2015-10-01 |
description |
Cotton fibre is multigenic trait controlled by number of genes. Previous studies suggest that one of these genes may be responsible for switching cotton fibre growth on and off to influence the fibre quality produced from a cotton seed. In the present study, the Gossypium hirsutum GhEXPA8 fibre expansin gene cloned into a pGA482 plant expression vector with a 2X 35S promoter and a CaMV terminator was introduced into local cotton variety NIAB 846 by using an Agrobacterium-mediated gene transformation. The neomycin phosphotransferase (NPTII) gene was used as a selection marker for screening of putative transgenic cotton plants. Integration and expression of the fibre expansin gene in cotton plants was confirmed with molecular techniques including Southern blot analyses, real-time PCR and cellulose assays. The data collected from three years of field performance of the transgenic cotton plants expressing GhEXPA8 showed that significant improvement has been made in fibre lengths and micronaire values as compared to control Gossypium hirsutum variety NIAB 846 cotton plants. Field data of fibre and morphological characteristics were also analyzed through statistical techniques. The results of this study support improvement of cotton fibre through genetic modification. |
topic |
Agrobacterium tumefaciens Cotton Fiber Transformation, Genetic Micronaire value GhEXPA8 gene |
url |
http://journal.frontiersin.org/Journal/10.3389/fpls.2015.00838/full |
work_keys_str_mv |
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