Environmental influence on Pristionchus pacificus mouth form through different culture methods
Abstract Environmental cues can impact development to elicit distinct phenotypes in the adult. The consequences of phenotypic plasticity can have profound effects on morphology, life cycle, and behavior to increase the fitness of the organism. The molecular mechanisms governing these interactions ar...
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Online Access: | https://doi.org/10.1038/s41598-017-07455-7 |
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doaj-913d8658c7ca4c279d1af3e55cb0a8002020-12-08T01:37:25ZengNature Publishing GroupScientific Reports2045-23222017-08-017111210.1038/s41598-017-07455-7Environmental influence on Pristionchus pacificus mouth form through different culture methodsMichael S. Werner0Bogdan Sieriebriennikov1Tobias Loschko2Suryesh Namdeo3Masa Lenuzzi4Mohannad Dardiry5Tess Renahan6Devansh Raj Sharma7Ralf J. Sommer8Department of Evolutionary Biology, Max Planck Institute for Developmental BiologyDepartment of Evolutionary Biology, Max Planck Institute for Developmental BiologyDepartment of Evolutionary Biology, Max Planck Institute for Developmental BiologyDepartment of Evolutionary Biology, Max Planck Institute for Developmental BiologyDepartment of Evolutionary Biology, Max Planck Institute for Developmental BiologyDepartment of Evolutionary Biology, Max Planck Institute for Developmental BiologyDepartment of Evolutionary Biology, Max Planck Institute for Developmental BiologyDepartment of Evolutionary Biology, Max Planck Institute for Developmental BiologyDepartment of Evolutionary Biology, Max Planck Institute for Developmental BiologyAbstract Environmental cues can impact development to elicit distinct phenotypes in the adult. The consequences of phenotypic plasticity can have profound effects on morphology, life cycle, and behavior to increase the fitness of the organism. The molecular mechanisms governing these interactions are beginning to be elucidated in a few cases, such as social insects. Nevertheless, there is a paucity of systems that are amenable to rigorous experimentation, preventing both detailed mechanistic insight and the establishment of a generalizable conceptual framework. The mouth dimorphism of the model nematode Pristionchus pacificus offers the rare opportunity to examine the genetics, genomics, and epigenetics of environmental influence on developmental plasticity. Yet there are currently no easily tunable environmental factors that affect mouth-form ratios and are scalable to large cultures required for molecular biology. Here we present a suite of culture conditions to toggle the mouth-form phenotype of P. pacificus. The effects are reversible, do not require the costly or labor-intensive synthesis of chemicals, and proceed through the same pathways previously examined from forward genetic screens. Different species of Pristionchus exhibit different responses to culture conditions, demonstrating unique gene-environment interactions, and providing an opportunity to study environmental influence on a macroevolutionary scale.https://doi.org/10.1038/s41598-017-07455-7 |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Michael S. Werner Bogdan Sieriebriennikov Tobias Loschko Suryesh Namdeo Masa Lenuzzi Mohannad Dardiry Tess Renahan Devansh Raj Sharma Ralf J. Sommer |
spellingShingle |
Michael S. Werner Bogdan Sieriebriennikov Tobias Loschko Suryesh Namdeo Masa Lenuzzi Mohannad Dardiry Tess Renahan Devansh Raj Sharma Ralf J. Sommer Environmental influence on Pristionchus pacificus mouth form through different culture methods Scientific Reports |
author_facet |
Michael S. Werner Bogdan Sieriebriennikov Tobias Loschko Suryesh Namdeo Masa Lenuzzi Mohannad Dardiry Tess Renahan Devansh Raj Sharma Ralf J. Sommer |
author_sort |
Michael S. Werner |
title |
Environmental influence on Pristionchus pacificus mouth form through different culture methods |
title_short |
Environmental influence on Pristionchus pacificus mouth form through different culture methods |
title_full |
Environmental influence on Pristionchus pacificus mouth form through different culture methods |
title_fullStr |
Environmental influence on Pristionchus pacificus mouth form through different culture methods |
title_full_unstemmed |
Environmental influence on Pristionchus pacificus mouth form through different culture methods |
title_sort |
environmental influence on pristionchus pacificus mouth form through different culture methods |
publisher |
Nature Publishing Group |
series |
Scientific Reports |
issn |
2045-2322 |
publishDate |
2017-08-01 |
description |
Abstract Environmental cues can impact development to elicit distinct phenotypes in the adult. The consequences of phenotypic plasticity can have profound effects on morphology, life cycle, and behavior to increase the fitness of the organism. The molecular mechanisms governing these interactions are beginning to be elucidated in a few cases, such as social insects. Nevertheless, there is a paucity of systems that are amenable to rigorous experimentation, preventing both detailed mechanistic insight and the establishment of a generalizable conceptual framework. The mouth dimorphism of the model nematode Pristionchus pacificus offers the rare opportunity to examine the genetics, genomics, and epigenetics of environmental influence on developmental plasticity. Yet there are currently no easily tunable environmental factors that affect mouth-form ratios and are scalable to large cultures required for molecular biology. Here we present a suite of culture conditions to toggle the mouth-form phenotype of P. pacificus. The effects are reversible, do not require the costly or labor-intensive synthesis of chemicals, and proceed through the same pathways previously examined from forward genetic screens. Different species of Pristionchus exhibit different responses to culture conditions, demonstrating unique gene-environment interactions, and providing an opportunity to study environmental influence on a macroevolutionary scale. |
url |
https://doi.org/10.1038/s41598-017-07455-7 |
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