Tris (1,3-dichloro-2-propyl) phosphate exposure disrupts the gut microbiome and its associated metabolites in mice
Background: Tris (1,3-dichloro-2-propyl) phosphate (TDCIPP) has been frequently detected in environmental media and biological samples. However, knowledge of its adverse health consequences is limited, and its impacts on the human gut microbiota, which play a key role in health and disease, remain u...
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doaj-913f10a4bb234cf484ba3392096091fb2020-12-27T04:27:52ZengElsevierEnvironment International0160-41202021-01-01146106256Tris (1,3-dichloro-2-propyl) phosphate exposure disrupts the gut microbiome and its associated metabolites in miceXiao Yan0Mian He1Jing Zheng2Ting Zhu3Zhongjie Zou4Bin Tang5Yunjiang Yu6Bixian Mai7State Environmental Protection Key Laboratory of Environmental Pollution Health Risk Assessment, South China Institute of Environmental Sciences, Ministry of Ecology and Environment, Guangzhou 510655, PR ChinaThe Seventh Affiliated Hospital of Sun Yat-sen University, Shenzhen 518107, PR ChinaState Environmental Protection Key Laboratory of Environmental Pollution Health Risk Assessment, South China Institute of Environmental Sciences, Ministry of Ecology and Environment, Guangzhou 510655, PR China; Corresponding authors.State Key Laboratory of Organic Geochemistry and Guangdong Key Laboratory of Environmental Resources Utilization and Protection, Guangzhou Institute of Geochemistry, Chinese Academy of Sciences, Guangzhou 510640, PR ChinaSchool of Traditional Chinese Medicine, Guangdong Pharmaceutical University, Guangzhou 510006, PR ChinaState Environmental Protection Key Laboratory of Environmental Pollution Health Risk Assessment, South China Institute of Environmental Sciences, Ministry of Ecology and Environment, Guangzhou 510655, PR ChinaState Environmental Protection Key Laboratory of Environmental Pollution Health Risk Assessment, South China Institute of Environmental Sciences, Ministry of Ecology and Environment, Guangzhou 510655, PR China; Corresponding authors.State Key Laboratory of Organic Geochemistry and Guangdong Key Laboratory of Environmental Resources Utilization and Protection, Guangzhou Institute of Geochemistry, Chinese Academy of Sciences, Guangzhou 510640, PR ChinaBackground: Tris (1,3-dichloro-2-propyl) phosphate (TDCIPP) has been frequently detected in environmental media and biological samples. However, knowledge of its adverse health consequences is limited, and its impacts on the human gut microbiota, which play a key role in health and disease, remain unexplored. Objectives: To better evaluate the potential risk of TDCIPP exposure in human health, we investigated the effects of TDCIPP on gut microbiome and gut metabolites in C57BL/6 mice. Methods: We applied an integrated analytical approach by combing 16S rRNA gene sequencing, metagenomic sequencing and 1H NMR metabolomics analysis in fecal samples collected from mouse with TDCIPP exposure as well as those from controls. Results: Both 16S rRNA sequencing and metagenome sequencing showed that TDCIPP exposure significantly changed the gut microbiome, with a remarkable increased Firmicutes at the expense of Bacteroidetes after exposure. Perturbed gut metabolic profiles in the treated group were also observed and closely related with altered gut microbiome. Gene functional annotation analysis further suggested perturbed gut metabolites could be directly caused by altered gut microbiome. Conclusion: TDCIPP exposure has great influence on the gut ecosystem as reflected by perturbation of microbiome community structure, microbial species, gut microbe associated gene expression and gut metabolites, which may contribute to the progression of certain uncharacterized gut microbiota related host diseases. Our findings provide novel insights into adverse effects of TDCIPP exposure on human health.http://www.sciencedirect.com/science/article/pii/S016041202032211XTris (13-dichloro-2-propyl) phosphateGut microbiomeGut metabolomicMice |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Xiao Yan Mian He Jing Zheng Ting Zhu Zhongjie Zou Bin Tang Yunjiang Yu Bixian Mai |
spellingShingle |
Xiao Yan Mian He Jing Zheng Ting Zhu Zhongjie Zou Bin Tang Yunjiang Yu Bixian Mai Tris (1,3-dichloro-2-propyl) phosphate exposure disrupts the gut microbiome and its associated metabolites in mice Environment International Tris (1 3-dichloro-2-propyl) phosphate Gut microbiome Gut metabolomic Mice |
author_facet |
Xiao Yan Mian He Jing Zheng Ting Zhu Zhongjie Zou Bin Tang Yunjiang Yu Bixian Mai |
author_sort |
Xiao Yan |
title |
Tris (1,3-dichloro-2-propyl) phosphate exposure disrupts the gut microbiome and its associated metabolites in mice |
title_short |
Tris (1,3-dichloro-2-propyl) phosphate exposure disrupts the gut microbiome and its associated metabolites in mice |
title_full |
Tris (1,3-dichloro-2-propyl) phosphate exposure disrupts the gut microbiome and its associated metabolites in mice |
title_fullStr |
Tris (1,3-dichloro-2-propyl) phosphate exposure disrupts the gut microbiome and its associated metabolites in mice |
title_full_unstemmed |
Tris (1,3-dichloro-2-propyl) phosphate exposure disrupts the gut microbiome and its associated metabolites in mice |
title_sort |
tris (1,3-dichloro-2-propyl) phosphate exposure disrupts the gut microbiome and its associated metabolites in mice |
publisher |
Elsevier |
series |
Environment International |
issn |
0160-4120 |
publishDate |
2021-01-01 |
description |
Background: Tris (1,3-dichloro-2-propyl) phosphate (TDCIPP) has been frequently detected in environmental media and biological samples. However, knowledge of its adverse health consequences is limited, and its impacts on the human gut microbiota, which play a key role in health and disease, remain unexplored. Objectives: To better evaluate the potential risk of TDCIPP exposure in human health, we investigated the effects of TDCIPP on gut microbiome and gut metabolites in C57BL/6 mice. Methods: We applied an integrated analytical approach by combing 16S rRNA gene sequencing, metagenomic sequencing and 1H NMR metabolomics analysis in fecal samples collected from mouse with TDCIPP exposure as well as those from controls. Results: Both 16S rRNA sequencing and metagenome sequencing showed that TDCIPP exposure significantly changed the gut microbiome, with a remarkable increased Firmicutes at the expense of Bacteroidetes after exposure. Perturbed gut metabolic profiles in the treated group were also observed and closely related with altered gut microbiome. Gene functional annotation analysis further suggested perturbed gut metabolites could be directly caused by altered gut microbiome. Conclusion: TDCIPP exposure has great influence on the gut ecosystem as reflected by perturbation of microbiome community structure, microbial species, gut microbe associated gene expression and gut metabolites, which may contribute to the progression of certain uncharacterized gut microbiota related host diseases. Our findings provide novel insights into adverse effects of TDCIPP exposure on human health. |
topic |
Tris (1 3-dichloro-2-propyl) phosphate Gut microbiome Gut metabolomic Mice |
url |
http://www.sciencedirect.com/science/article/pii/S016041202032211X |
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