Anti-SARS-Cov-2 IgA Response in Tears of COVID-19 Patients

The pandemic virus SARS-CoV-2 has been reported to be able to enter the body via the eye conjunctiva, but the presence of antiviral response in the eye remains poorly known. Our study was thus aimed to analyze the presence of secretory mucosal anti-SARS-CoV-2 type A immunoglobulins (IgA) in the conj...

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Main Authors: Elisabetta Caselli, Irene Soffritti, Giuseppe Lamberti, Maria D’Accolti, Filippo Franco, Davide Demaria, Marco Contoli, Angela Passaro, Carlo Contini, Paolo Perri
Format: Article
Language:English
Published: MDPI AG 2020-11-01
Series:Biology
Subjects:
IgA
eye
Online Access:https://www.mdpi.com/2079-7737/9/11/374
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spelling doaj-913f81cf22db4a5c9dbf7727d42ab0d62020-11-25T04:04:07ZengMDPI AGBiology2079-77372020-11-01937437410.3390/biology9110374Anti-SARS-Cov-2 IgA Response in Tears of COVID-19 PatientsElisabetta Caselli0Irene Soffritti1Giuseppe Lamberti2Maria D’Accolti3Filippo Franco4Davide Demaria5Marco Contoli6Angela Passaro7Carlo Contini8Paolo Perri9Department of Chemical and Pharmaceutical Sciences and LTTA, Section of Microbiology, University of Ferrara, 44121 Ferrara, ItalyDepartment of Chemical and Pharmaceutical Sciences and LTTA, Section of Microbiology, University of Ferrara, 44121 Ferrara, ItalyDepartment of Biomedical and Surgical-Specialized Sciences, Ophthalmology Unit, University of Ferrara, 44121 Ferrara, ItalyDepartment of Chemical and Pharmaceutical Sciences and LTTA, Section of Microbiology, University of Ferrara, 44121 Ferrara, ItalyDepartment of Biomedical and Surgical-Specialized Sciences, Ophthalmology Unit, University of Ferrara, 44121 Ferrara, ItalyDepartment of Biomedical and Surgical-Specialized Sciences, Ophthalmology Unit, University of Ferrara, 44121 Ferrara, ItalyDepartment of Morphology, Surgery and Experimental Medicine, University of Ferrara, 44121 Ferrara, ItalyDepartment of Morphology, Surgery and Experimental Medicine, University of Ferrara, 44121 Ferrara, ItalyDepartment of Medical Sciences, Infectious Diseases Unit, University of Ferrara, 44121 Ferrara, ItalyDepartment of Morphology, Surgery and Experimental Medicine, University of Ferrara, 44121 Ferrara, ItalyThe pandemic virus SARS-CoV-2 has been reported to be able to enter the body via the eye conjunctiva, but the presence of antiviral response in the eye remains poorly known. Our study was thus aimed to analyze the presence of secretory mucosal anti-SARS-CoV-2 type A immunoglobulins (IgA) in the conjunctival fluid of COVID-19 patients. The tears of 28 COVID-19 patients and 20 uninfected controls were collected by the Schirmer test and analyzed by a specific ELISA assay detecting anti-spike (S1) virus protein IgA. The results showed that 35.7% of COVID-19 subjects have specific antiviral IgA at the ocular level, persisting till 48 days post disease onset. Most of the IgA positive subjects presented mild symptoms. The collected data indicate a prolonged persistence of anti-SARS-CoV-2 IgA at the eye level and suggest that IgA detection may be extremely helpful in clarifying virus pathology and epidemiology.https://www.mdpi.com/2079-7737/9/11/374SAR-CoV-2IgAeyeCOVID-19
collection DOAJ
language English
format Article
sources DOAJ
author Elisabetta Caselli
Irene Soffritti
Giuseppe Lamberti
Maria D’Accolti
Filippo Franco
Davide Demaria
Marco Contoli
Angela Passaro
Carlo Contini
Paolo Perri
spellingShingle Elisabetta Caselli
Irene Soffritti
Giuseppe Lamberti
Maria D’Accolti
Filippo Franco
Davide Demaria
Marco Contoli
Angela Passaro
Carlo Contini
Paolo Perri
Anti-SARS-Cov-2 IgA Response in Tears of COVID-19 Patients
Biology
SAR-CoV-2
IgA
eye
COVID-19
author_facet Elisabetta Caselli
Irene Soffritti
Giuseppe Lamberti
Maria D’Accolti
Filippo Franco
Davide Demaria
Marco Contoli
Angela Passaro
Carlo Contini
Paolo Perri
author_sort Elisabetta Caselli
title Anti-SARS-Cov-2 IgA Response in Tears of COVID-19 Patients
title_short Anti-SARS-Cov-2 IgA Response in Tears of COVID-19 Patients
title_full Anti-SARS-Cov-2 IgA Response in Tears of COVID-19 Patients
title_fullStr Anti-SARS-Cov-2 IgA Response in Tears of COVID-19 Patients
title_full_unstemmed Anti-SARS-Cov-2 IgA Response in Tears of COVID-19 Patients
title_sort anti-sars-cov-2 iga response in tears of covid-19 patients
publisher MDPI AG
series Biology
issn 2079-7737
publishDate 2020-11-01
description The pandemic virus SARS-CoV-2 has been reported to be able to enter the body via the eye conjunctiva, but the presence of antiviral response in the eye remains poorly known. Our study was thus aimed to analyze the presence of secretory mucosal anti-SARS-CoV-2 type A immunoglobulins (IgA) in the conjunctival fluid of COVID-19 patients. The tears of 28 COVID-19 patients and 20 uninfected controls were collected by the Schirmer test and analyzed by a specific ELISA assay detecting anti-spike (S1) virus protein IgA. The results showed that 35.7% of COVID-19 subjects have specific antiviral IgA at the ocular level, persisting till 48 days post disease onset. Most of the IgA positive subjects presented mild symptoms. The collected data indicate a prolonged persistence of anti-SARS-CoV-2 IgA at the eye level and suggest that IgA detection may be extremely helpful in clarifying virus pathology and epidemiology.
topic SAR-CoV-2
IgA
eye
COVID-19
url https://www.mdpi.com/2079-7737/9/11/374
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