Anti-SARS-Cov-2 IgA Response in Tears of COVID-19 Patients
The pandemic virus SARS-CoV-2 has been reported to be able to enter the body via the eye conjunctiva, but the presence of antiviral response in the eye remains poorly known. Our study was thus aimed to analyze the presence of secretory mucosal anti-SARS-CoV-2 type A immunoglobulins (IgA) in the conj...
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doaj-913f81cf22db4a5c9dbf7727d42ab0d62020-11-25T04:04:07ZengMDPI AGBiology2079-77372020-11-01937437410.3390/biology9110374Anti-SARS-Cov-2 IgA Response in Tears of COVID-19 PatientsElisabetta Caselli0Irene Soffritti1Giuseppe Lamberti2Maria D’Accolti3Filippo Franco4Davide Demaria5Marco Contoli6Angela Passaro7Carlo Contini8Paolo Perri9Department of Chemical and Pharmaceutical Sciences and LTTA, Section of Microbiology, University of Ferrara, 44121 Ferrara, ItalyDepartment of Chemical and Pharmaceutical Sciences and LTTA, Section of Microbiology, University of Ferrara, 44121 Ferrara, ItalyDepartment of Biomedical and Surgical-Specialized Sciences, Ophthalmology Unit, University of Ferrara, 44121 Ferrara, ItalyDepartment of Chemical and Pharmaceutical Sciences and LTTA, Section of Microbiology, University of Ferrara, 44121 Ferrara, ItalyDepartment of Biomedical and Surgical-Specialized Sciences, Ophthalmology Unit, University of Ferrara, 44121 Ferrara, ItalyDepartment of Biomedical and Surgical-Specialized Sciences, Ophthalmology Unit, University of Ferrara, 44121 Ferrara, ItalyDepartment of Morphology, Surgery and Experimental Medicine, University of Ferrara, 44121 Ferrara, ItalyDepartment of Morphology, Surgery and Experimental Medicine, University of Ferrara, 44121 Ferrara, ItalyDepartment of Medical Sciences, Infectious Diseases Unit, University of Ferrara, 44121 Ferrara, ItalyDepartment of Morphology, Surgery and Experimental Medicine, University of Ferrara, 44121 Ferrara, ItalyThe pandemic virus SARS-CoV-2 has been reported to be able to enter the body via the eye conjunctiva, but the presence of antiviral response in the eye remains poorly known. Our study was thus aimed to analyze the presence of secretory mucosal anti-SARS-CoV-2 type A immunoglobulins (IgA) in the conjunctival fluid of COVID-19 patients. The tears of 28 COVID-19 patients and 20 uninfected controls were collected by the Schirmer test and analyzed by a specific ELISA assay detecting anti-spike (S1) virus protein IgA. The results showed that 35.7% of COVID-19 subjects have specific antiviral IgA at the ocular level, persisting till 48 days post disease onset. Most of the IgA positive subjects presented mild symptoms. The collected data indicate a prolonged persistence of anti-SARS-CoV-2 IgA at the eye level and suggest that IgA detection may be extremely helpful in clarifying virus pathology and epidemiology.https://www.mdpi.com/2079-7737/9/11/374SAR-CoV-2IgAeyeCOVID-19 |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Elisabetta Caselli Irene Soffritti Giuseppe Lamberti Maria D’Accolti Filippo Franco Davide Demaria Marco Contoli Angela Passaro Carlo Contini Paolo Perri |
spellingShingle |
Elisabetta Caselli Irene Soffritti Giuseppe Lamberti Maria D’Accolti Filippo Franco Davide Demaria Marco Contoli Angela Passaro Carlo Contini Paolo Perri Anti-SARS-Cov-2 IgA Response in Tears of COVID-19 Patients Biology SAR-CoV-2 IgA eye COVID-19 |
author_facet |
Elisabetta Caselli Irene Soffritti Giuseppe Lamberti Maria D’Accolti Filippo Franco Davide Demaria Marco Contoli Angela Passaro Carlo Contini Paolo Perri |
author_sort |
Elisabetta Caselli |
title |
Anti-SARS-Cov-2 IgA Response in Tears of COVID-19 Patients |
title_short |
Anti-SARS-Cov-2 IgA Response in Tears of COVID-19 Patients |
title_full |
Anti-SARS-Cov-2 IgA Response in Tears of COVID-19 Patients |
title_fullStr |
Anti-SARS-Cov-2 IgA Response in Tears of COVID-19 Patients |
title_full_unstemmed |
Anti-SARS-Cov-2 IgA Response in Tears of COVID-19 Patients |
title_sort |
anti-sars-cov-2 iga response in tears of covid-19 patients |
publisher |
MDPI AG |
series |
Biology |
issn |
2079-7737 |
publishDate |
2020-11-01 |
description |
The pandemic virus SARS-CoV-2 has been reported to be able to enter the body via the eye conjunctiva, but the presence of antiviral response in the eye remains poorly known. Our study was thus aimed to analyze the presence of secretory mucosal anti-SARS-CoV-2 type A immunoglobulins (IgA) in the conjunctival fluid of COVID-19 patients. The tears of 28 COVID-19 patients and 20 uninfected controls were collected by the Schirmer test and analyzed by a specific ELISA assay detecting anti-spike (S1) virus protein IgA. The results showed that 35.7% of COVID-19 subjects have specific antiviral IgA at the ocular level, persisting till 48 days post disease onset. Most of the IgA positive subjects presented mild symptoms. The collected data indicate a prolonged persistence of anti-SARS-CoV-2 IgA at the eye level and suggest that IgA detection may be extremely helpful in clarifying virus pathology and epidemiology. |
topic |
SAR-CoV-2 IgA eye COVID-19 |
url |
https://www.mdpi.com/2079-7737/9/11/374 |
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