Linker and N-Terminal Domain Engineering of Pyrrolysyl-tRNA Synthetase for Substrate Range Shifting and Activity Enhancement

Linker and N-Terminal Domain Engineering of Pyrrolysyl-tRNA Synthetase for Substrate Range Shifting and Activity Enhancement

The Methanosarcina mazei pyrrolysyl-tRNA synthetase (PylRS)⋅tRNAPyl pair can be used to incorporate non-canonical amino acids (ncAAs) into proteins at installed amber stop codons. Although engineering of the PylRS active site generates diverse binding pockets, the substrate ranges are found similar...

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Bibliographic Details
Main Authors: Han-Kai Jiang, Man-Nee Lee, Jo-Chu Tsou, Kuan-Wen Chang, Hsueh-Wei Tseng, Kuang-Po Chen, Yaw-Kuen Li, Yane-Shih Wang
Format: Article
Language:English
Published: Frontiers Media S.A. 2020-04-01
Series:Frontiers in Bioengineering and Biotechnology
Subjects:
non-canonical amino acids
pyrrolysyl-tRNA synthetase
linker engineering
amber codon suppression
tRNA binding domain
Online Access:https://www.frontiersin.org/article/10.3389/fbioe.2020.00235/full

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https://www.frontiersin.org/article/10.3389/fbioe.2020.00235/full

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