Determination of cholesterol at the low picomole level by nano-electrospray ionization tandem mass spectrometry
A mass spectrometric method for the quantification of free cholesterol in cells and subcellular membranes is presented. The method is based on a simple one-step chemical derivatization of cholesterol to cholesterol-3-sulfate by a sulfur trioxide-pyridine complex. Quantification is performed by nano-...
Main Authors: | Roger Sandhoff, Britta Brügger, Dieter Jeckel, Wolf D. Lehmann, Felix T. Wieland |
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Format: | Article |
Language: | English |
Published: |
Elsevier
1999-01-01
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Series: | Journal of Lipid Research |
Subjects: | |
Online Access: | http://www.sciencedirect.com/science/article/pii/S0022227520333472 |
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