Fabrication and Characterization of Hydrogels Based on Gelatinised Collagen with Potential Application in Tissue Engineering

Regenerative medicine is increasingly focused on the development of biomaterials that facilitate cell adhesion and proliferation through the use of natural polymers, which have better biocompatibility and biodegradability. In this way, the use of hydrogels has been considered as a potential option f...

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Main Authors: Victor Perez-Puyana, Mercedes Jiménez-Rosado, Alberto Romero, Antonio Guerrero
Format: Article
Language:English
Published: MDPI AG 2020-05-01
Series:Polymers
Subjects:
Online Access:https://www.mdpi.com/2073-4360/12/5/1146
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spelling doaj-916bba04a7a54ebb92c7935a487988902020-11-25T03:00:21ZengMDPI AGPolymers2073-43602020-05-01121146114610.3390/polym12051146Fabrication and Characterization of Hydrogels Based on Gelatinised Collagen with Potential Application in Tissue EngineeringVictor Perez-Puyana0Mercedes Jiménez-Rosado1Alberto Romero2Antonio Guerrero3Departamento de Ingeniería Química, Facultad de Química, Universidad de Sevilla, 41012 Sevilla, SpainDepartamento de Ingeniería Química, Escuela Politécnica Superior, Universidad de Sevilla, 41011 Sevilla, SpainDepartamento de Ingeniería Química, Facultad de Química, Universidad de Sevilla, 41012 Sevilla, SpainDepartamento de Ingeniería Química, Escuela Politécnica Superior, Universidad de Sevilla, 41011 Sevilla, SpainRegenerative medicine is increasingly focused on the development of biomaterials that facilitate cell adhesion and proliferation through the use of natural polymers, which have better biocompatibility and biodegradability. In this way, the use of hydrogels has been considered as a potential option for tissue engineering due to their physical and chemical characteristics. However, few studies associate the raw materials properties and processing conditions with the final characteristics of hydrogels, which could condition their use as scaffolds for tissue engineering. In this context, the main objective of this work was the evaluation of type I collagen as raw material for the elaboration of hydrogels. In addition, gelation time, pH and temperature were evaluated as the most influential variables in the hydrogel processing method by rheological (time, strain and frequency sweep tests) and microstructural (Cryo-SEM) measurements. The results indicate that it is possible to obtain collagen hydrogels with adequate rheological and microstructural characteristics by selecting optimal processing conditions. However, further studies are necessary to assess their suitability for cell accommodation and growth.https://www.mdpi.com/2073-4360/12/5/1146gelatinised collagenhydrogelrheologyCryo-SEMtissue engineering
collection DOAJ
language English
format Article
sources DOAJ
author Victor Perez-Puyana
Mercedes Jiménez-Rosado
Alberto Romero
Antonio Guerrero
spellingShingle Victor Perez-Puyana
Mercedes Jiménez-Rosado
Alberto Romero
Antonio Guerrero
Fabrication and Characterization of Hydrogels Based on Gelatinised Collagen with Potential Application in Tissue Engineering
Polymers
gelatinised collagen
hydrogel
rheology
Cryo-SEM
tissue engineering
author_facet Victor Perez-Puyana
Mercedes Jiménez-Rosado
Alberto Romero
Antonio Guerrero
author_sort Victor Perez-Puyana
title Fabrication and Characterization of Hydrogels Based on Gelatinised Collagen with Potential Application in Tissue Engineering
title_short Fabrication and Characterization of Hydrogels Based on Gelatinised Collagen with Potential Application in Tissue Engineering
title_full Fabrication and Characterization of Hydrogels Based on Gelatinised Collagen with Potential Application in Tissue Engineering
title_fullStr Fabrication and Characterization of Hydrogels Based on Gelatinised Collagen with Potential Application in Tissue Engineering
title_full_unstemmed Fabrication and Characterization of Hydrogels Based on Gelatinised Collagen with Potential Application in Tissue Engineering
title_sort fabrication and characterization of hydrogels based on gelatinised collagen with potential application in tissue engineering
publisher MDPI AG
series Polymers
issn 2073-4360
publishDate 2020-05-01
description Regenerative medicine is increasingly focused on the development of biomaterials that facilitate cell adhesion and proliferation through the use of natural polymers, which have better biocompatibility and biodegradability. In this way, the use of hydrogels has been considered as a potential option for tissue engineering due to their physical and chemical characteristics. However, few studies associate the raw materials properties and processing conditions with the final characteristics of hydrogels, which could condition their use as scaffolds for tissue engineering. In this context, the main objective of this work was the evaluation of type I collagen as raw material for the elaboration of hydrogels. In addition, gelation time, pH and temperature were evaluated as the most influential variables in the hydrogel processing method by rheological (time, strain and frequency sweep tests) and microstructural (Cryo-SEM) measurements. The results indicate that it is possible to obtain collagen hydrogels with adequate rheological and microstructural characteristics by selecting optimal processing conditions. However, further studies are necessary to assess their suitability for cell accommodation and growth.
topic gelatinised collagen
hydrogel
rheology
Cryo-SEM
tissue engineering
url https://www.mdpi.com/2073-4360/12/5/1146
work_keys_str_mv AT victorperezpuyana fabricationandcharacterizationofhydrogelsbasedongelatinisedcollagenwithpotentialapplicationintissueengineering
AT mercedesjimenezrosado fabricationandcharacterizationofhydrogelsbasedongelatinisedcollagenwithpotentialapplicationintissueengineering
AT albertoromero fabricationandcharacterizationofhydrogelsbasedongelatinisedcollagenwithpotentialapplicationintissueengineering
AT antonioguerrero fabricationandcharacterizationofhydrogelsbasedongelatinisedcollagenwithpotentialapplicationintissueengineering
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