Variance decomposition of protein profiles from antibody arrays using a longitudinal twin model
<p>Abstract</p> <p>Background</p> <p>The advent of affinity-based proteomics technologies for global protein profiling provides the prospect of finding new molecular biomarkers for common, multifactorial disorders. The molecular phenotypes obtained from studies on such...
Main Authors: | , , , , , , , , , |
---|---|
Format: | Article |
Language: | English |
Published: |
BMC
2011-11-01
|
Series: | Proteome Science |
Subjects: | |
Online Access: | http://www.proteomesci.com/content/9/1/73 |
id |
doaj-9171ed74803348909081c327bb97a0f0 |
---|---|
record_format |
Article |
spelling |
doaj-9171ed74803348909081c327bb97a0f02020-11-25T02:45:13ZengBMCProteome Science1477-59562011-11-01917310.1186/1477-5956-9-73Variance decomposition of protein profiles from antibody arrays using a longitudinal twin modelKato Bernet SNicholson GeorgeNeiman MajaRantalainen MattiasHolmes Chris CBarrett AmyUhlén MathiasNilsson PeterSpector Tim DSchwenk Jochen M<p>Abstract</p> <p>Background</p> <p>The advent of affinity-based proteomics technologies for global protein profiling provides the prospect of finding new molecular biomarkers for common, multifactorial disorders. The molecular phenotypes obtained from studies on such platforms are driven by multiple sources, including genetic, environmental, and experimental components. In characterizing the contribution of different sources of variation to the measured phenotypes, the aim is to facilitate the design and interpretation of future biomedical studies employing exploratory and multiplexed technologies. Thus, biometrical genetic modelling of twin or other family data can be used to decompose the variation underlying a phenotype into biological and experimental components.</p> <p>Results</p> <p>Using antibody suspension bead arrays and antibodies from the Human Protein Atlas, we study unfractionated serum from a longitudinal study on 154 twins. In this study, we provide a detailed description of how the variation in a molecular phenotype in terms of protein profile can be decomposed into familial i.e. genetic and common environmental; individual environmental, short-term biological and experimental components. The results show that across 69 antibodies analyzed in the study, the median proportion of the total variation explained by familial sources is 12% (IQR 1-22%), and the median proportion of the total variation attributable to experimental sources is 63% (IQR 53-72%).</p> <p>Conclusion</p> <p>The variability analysis of antibody arrays highlights the importance to consider variability components and their relative contributions when designing and evaluating studies for biomarker discoveries with exploratory, high-throughput and multiplexed methods.</p> http://www.proteomesci.com/content/9/1/73Variance decompositionlinear mixed-effects modellongitudinal twin studysuspension bead arraysantibodiesprotein profiling |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Kato Bernet S Nicholson George Neiman Maja Rantalainen Mattias Holmes Chris C Barrett Amy Uhlén Mathias Nilsson Peter Spector Tim D Schwenk Jochen M |
spellingShingle |
Kato Bernet S Nicholson George Neiman Maja Rantalainen Mattias Holmes Chris C Barrett Amy Uhlén Mathias Nilsson Peter Spector Tim D Schwenk Jochen M Variance decomposition of protein profiles from antibody arrays using a longitudinal twin model Proteome Science Variance decomposition linear mixed-effects model longitudinal twin study suspension bead arrays antibodies protein profiling |
author_facet |
Kato Bernet S Nicholson George Neiman Maja Rantalainen Mattias Holmes Chris C Barrett Amy Uhlén Mathias Nilsson Peter Spector Tim D Schwenk Jochen M |
author_sort |
Kato Bernet S |
title |
Variance decomposition of protein profiles from antibody arrays using a longitudinal twin model |
title_short |
Variance decomposition of protein profiles from antibody arrays using a longitudinal twin model |
title_full |
Variance decomposition of protein profiles from antibody arrays using a longitudinal twin model |
title_fullStr |
Variance decomposition of protein profiles from antibody arrays using a longitudinal twin model |
title_full_unstemmed |
Variance decomposition of protein profiles from antibody arrays using a longitudinal twin model |
title_sort |
variance decomposition of protein profiles from antibody arrays using a longitudinal twin model |
publisher |
BMC |
series |
Proteome Science |
issn |
1477-5956 |
publishDate |
2011-11-01 |
description |
<p>Abstract</p> <p>Background</p> <p>The advent of affinity-based proteomics technologies for global protein profiling provides the prospect of finding new molecular biomarkers for common, multifactorial disorders. The molecular phenotypes obtained from studies on such platforms are driven by multiple sources, including genetic, environmental, and experimental components. In characterizing the contribution of different sources of variation to the measured phenotypes, the aim is to facilitate the design and interpretation of future biomedical studies employing exploratory and multiplexed technologies. Thus, biometrical genetic modelling of twin or other family data can be used to decompose the variation underlying a phenotype into biological and experimental components.</p> <p>Results</p> <p>Using antibody suspension bead arrays and antibodies from the Human Protein Atlas, we study unfractionated serum from a longitudinal study on 154 twins. In this study, we provide a detailed description of how the variation in a molecular phenotype in terms of protein profile can be decomposed into familial i.e. genetic and common environmental; individual environmental, short-term biological and experimental components. The results show that across 69 antibodies analyzed in the study, the median proportion of the total variation explained by familial sources is 12% (IQR 1-22%), and the median proportion of the total variation attributable to experimental sources is 63% (IQR 53-72%).</p> <p>Conclusion</p> <p>The variability analysis of antibody arrays highlights the importance to consider variability components and their relative contributions when designing and evaluating studies for biomarker discoveries with exploratory, high-throughput and multiplexed methods.</p> |
topic |
Variance decomposition linear mixed-effects model longitudinal twin study suspension bead arrays antibodies protein profiling |
url |
http://www.proteomesci.com/content/9/1/73 |
work_keys_str_mv |
AT katobernets variancedecompositionofproteinprofilesfromantibodyarraysusingalongitudinaltwinmodel AT nicholsongeorge variancedecompositionofproteinprofilesfromantibodyarraysusingalongitudinaltwinmodel AT neimanmaja variancedecompositionofproteinprofilesfromantibodyarraysusingalongitudinaltwinmodel AT rantalainenmattias variancedecompositionofproteinprofilesfromantibodyarraysusingalongitudinaltwinmodel AT holmeschrisc variancedecompositionofproteinprofilesfromantibodyarraysusingalongitudinaltwinmodel AT barrettamy variancedecompositionofproteinprofilesfromantibodyarraysusingalongitudinaltwinmodel AT uhlenmathias variancedecompositionofproteinprofilesfromantibodyarraysusingalongitudinaltwinmodel AT nilssonpeter variancedecompositionofproteinprofilesfromantibodyarraysusingalongitudinaltwinmodel AT spectortimd variancedecompositionofproteinprofilesfromantibodyarraysusingalongitudinaltwinmodel AT schwenkjochenm variancedecompositionofproteinprofilesfromantibodyarraysusingalongitudinaltwinmodel |
_version_ |
1724763454814814208 |