| Summary: | A Gram-negative, motile, rod-shaped bacteria, designated D7<sup>T</sup>, was isolated by using the dilution-to-extinction method, from a soil sample taken from Rambla Salada (Murcia, Spain). Growth of strain D7<sup>T</sup> was observed at 15–40 °C (optimum, 37 °C), pH 5–9 (optimum, 7) and 0–7.5% (<i>w</i>/<i>v</i>) NaCl (optimum, 3%). It is facultatively anaerobic. Phylogenetic analysis based on 16S rRNA gene sequence showed it belongs to the genus <i>Marinobacterium</i>. The in silico DDH and ANI against closest <i>Marinobacterium</i> relatives support its placement as a new species within this genus. The major fatty acids of strain D7<sup>T</sup> were C<sub>16:0</sub>, summed feature 3 (C<sub>16:1</sub> <i>ω</i>7<i>c</i>/C<sub>16:1</sub> <i>ω</i>6<i>c</i>) and summed feature 8 (C<sub>18:1</sub> <i>ω</i>7<i>c</i>/C<sub>18:1</sub> <i>ω</i>6<i>c</i>). The polar lipid profile consists of phosphatidylethanolamine, phosphatidylglycerol and two uncharacterized lipids. Ubiquinone 8 was the unique isoprenoid quinone detected. The DNA G + C content was 59.2 mol%. On the basis of the phylogenetic, phenotypic, chemotaxonomic and genomic characterization, strain D7<sup>T</sup> (= CECT 9818<sup>T</sup> = LMG 31312<sup>T</sup>) represents a novel species of the genus <i>Marinobacterium</i> for which the name <i>Marinobacterium ramblicola</i> sp. nov. is proposed. Genome-based metabolic reconstructions of strain D7<sup>T</sup> suggested a heterotrophic and chemolitotrophic lifestyle, as well as the capacity to biosynthetize and catabolize compatible solutes, and to degrade hydrocarbon aromatic compounds.
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