Taxogenomic and Metabolic Insights into <i>Marinobacterium ramblicola</i> sp. nov., a New Slightly Halophilic Bacterium Isolated from Rambla Salada, Murcia

• Main Authors: Ana Durán-Viseras, David J. Castro, José Carlos Reina, Victoria Béjar, Fernando Martínez-Checa
• Format: Article
• Language: English
• Published: MDPI AG 2021-08-01
• Series: Microorganisms
• Subjects: <i>Marinobacterium</i>; hypersaline soil; taxogenomics; metabolism; slightly halophilic
• Online Access: https://www.mdpi.com/2076-2607/9/8/1654

A Gram-negative, motile, rod-shaped bacteria, designated D7^T, was isolated by using the dilution-to-extinction method, from a soil sample taken from Rambla Salada (Murcia, Spain). Growth of strain D7^T was observed at 15–40 °C (optimum, 37 °C), pH 5–9 (optimum, 7) and 0–7.5% (<i>w</i>/<i>v</i>) NaCl (optimum, 3%). It is facultatively anaerobic. Phylogenetic analysis based on 16S rRNA gene sequence showed it belongs to the genus <i>Marinobacterium</i>. The in silico DDH and ANI against closest <i>Marinobacterium</i> relatives support its placement as a new species within this genus. The major fatty acids of strain D7^T were C_16:0, summed feature 3 (C_16:1 <i>ω</i>7<i>c</i>/C_16:1 <i>ω</i>6<i>c</i>) and summed feature 8 (C_18:1 <i>ω</i>7<i>c</i>/C_18:1 <i>ω</i>6<i>c</i>). The polar lipid profile consists of phosphatidylethanolamine, phosphatidylglycerol and two uncharacterized lipids. Ubiquinone 8 was the unique isoprenoid quinone detected. The DNA G + C content was 59.2 mol%. On the basis of the phylogenetic, phenotypic, chemotaxonomic and genomic characterization, strain D7^T (= CECT 9818^T = LMG 31312^T) represents a novel species of the genus <i>Marinobacterium</i> for which the name <i>Marinobacterium ramblicola</i> sp. nov. is proposed. Genome-based metabolic reconstructions of strain D7^T suggested a heterotrophic and chemolitotrophic lifestyle, as well as the capacity to biosynthetize and catabolize compatible solutes, and to degrade hydrocarbon aromatic compounds.