The impact of CdSe/ZnS Quantum Dots in cells of <it>Medicago sativa </it>in suspension culture

<p>Abstract</p> <p>Background</p> <p>Nanotechnology has the potential to provide agriculture with new tools that may be used in the rapid detection and molecular treatment of diseases and enhancement of plant ability to absorb nutrients, among others. Data on nanopartic...

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Main Authors: Maycock Christopher, Malhó Rui, Tomaz Leonor, Miguel Ana S, Santos Ana R, Vaz Patto Maria C, Fevereiro Pedro, Oliva Abel
Format: Article
Language:English
Published: BMC 2010-10-01
Series:Journal of Nanobiotechnology
Online Access:http://www.jnanobiotechnology.com/content/8/1/24
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spelling doaj-918c27aadb1448bbaae7da96afa76fe62020-11-25T02:42:24ZengBMCJournal of Nanobiotechnology1477-31552010-10-01812410.1186/1477-3155-8-24The impact of CdSe/ZnS Quantum Dots in cells of <it>Medicago sativa </it>in suspension cultureMaycock ChristopherMalhó RuiTomaz LeonorMiguel Ana SSantos Ana RVaz Patto Maria CFevereiro PedroOliva Abel<p>Abstract</p> <p>Background</p> <p>Nanotechnology has the potential to provide agriculture with new tools that may be used in the rapid detection and molecular treatment of diseases and enhancement of plant ability to absorb nutrients, among others. Data on nanoparticle toxicity in plants is largely heterogeneous with a diversity of physicochemical parameters reported, which difficult generalizations. Here a cell biology approach was used to evaluate the impact of Quantum Dots (QDs) nanocrystals on plant cells, including their effect on cell growth, cell viability, oxidative stress and ROS accumulation, besides their cytomobility.</p> <p>Results</p> <p>A plant cell suspension culture of <it>Medicago sativa </it>was settled for the assessment of the impact of the addition of mercaptopropanoic acid coated CdSe/ZnS QDs. Cell growth was significantly reduced when 100 mM of mercaptopropanoic acid -QDs was added during the exponential growth phase, with less than 50% of the cells viable 72 hours after mercaptopropanoic acid -QDs addition. They were up taken by <it>Medicago sativa </it>cells and accumulated in the cytoplasm and nucleus as revealed by optical thin confocal imaging. As part of the cellular response to internalization, <it>Medicago sativa </it>cells were found to increase the production of Reactive Oxygen Species (ROS) in a dose and time dependent manner. Using the fluorescent dye H<sub>2</sub>DCFDA it was observable that mercaptopropanoic acid-QDs concentrations between 5-180 nM led to a progressive and linear increase of ROS accumulation.</p> <p>Conclusions</p> <p>Our results showed that the extent of mercaptopropanoic acid coated CdSe/ZnS QDs cytotoxicity in plant cells is dependent upon a number of factors including QDs properties, dose and the environmental conditions of administration and that, for <it>Medicago sativa </it>cells, a safe range of 1-5 nM should not be exceeded for biological applications.</p> http://www.jnanobiotechnology.com/content/8/1/24
collection DOAJ
language English
format Article
sources DOAJ
author Maycock Christopher
Malhó Rui
Tomaz Leonor
Miguel Ana S
Santos Ana R
Vaz Patto Maria C
Fevereiro Pedro
Oliva Abel
spellingShingle Maycock Christopher
Malhó Rui
Tomaz Leonor
Miguel Ana S
Santos Ana R
Vaz Patto Maria C
Fevereiro Pedro
Oliva Abel
The impact of CdSe/ZnS Quantum Dots in cells of <it>Medicago sativa </it>in suspension culture
Journal of Nanobiotechnology
author_facet Maycock Christopher
Malhó Rui
Tomaz Leonor
Miguel Ana S
Santos Ana R
Vaz Patto Maria C
Fevereiro Pedro
Oliva Abel
author_sort Maycock Christopher
title The impact of CdSe/ZnS Quantum Dots in cells of <it>Medicago sativa </it>in suspension culture
title_short The impact of CdSe/ZnS Quantum Dots in cells of <it>Medicago sativa </it>in suspension culture
title_full The impact of CdSe/ZnS Quantum Dots in cells of <it>Medicago sativa </it>in suspension culture
title_fullStr The impact of CdSe/ZnS Quantum Dots in cells of <it>Medicago sativa </it>in suspension culture
title_full_unstemmed The impact of CdSe/ZnS Quantum Dots in cells of <it>Medicago sativa </it>in suspension culture
title_sort impact of cdse/zns quantum dots in cells of <it>medicago sativa </it>in suspension culture
publisher BMC
series Journal of Nanobiotechnology
issn 1477-3155
publishDate 2010-10-01
description <p>Abstract</p> <p>Background</p> <p>Nanotechnology has the potential to provide agriculture with new tools that may be used in the rapid detection and molecular treatment of diseases and enhancement of plant ability to absorb nutrients, among others. Data on nanoparticle toxicity in plants is largely heterogeneous with a diversity of physicochemical parameters reported, which difficult generalizations. Here a cell biology approach was used to evaluate the impact of Quantum Dots (QDs) nanocrystals on plant cells, including their effect on cell growth, cell viability, oxidative stress and ROS accumulation, besides their cytomobility.</p> <p>Results</p> <p>A plant cell suspension culture of <it>Medicago sativa </it>was settled for the assessment of the impact of the addition of mercaptopropanoic acid coated CdSe/ZnS QDs. Cell growth was significantly reduced when 100 mM of mercaptopropanoic acid -QDs was added during the exponential growth phase, with less than 50% of the cells viable 72 hours after mercaptopropanoic acid -QDs addition. They were up taken by <it>Medicago sativa </it>cells and accumulated in the cytoplasm and nucleus as revealed by optical thin confocal imaging. As part of the cellular response to internalization, <it>Medicago sativa </it>cells were found to increase the production of Reactive Oxygen Species (ROS) in a dose and time dependent manner. Using the fluorescent dye H<sub>2</sub>DCFDA it was observable that mercaptopropanoic acid-QDs concentrations between 5-180 nM led to a progressive and linear increase of ROS accumulation.</p> <p>Conclusions</p> <p>Our results showed that the extent of mercaptopropanoic acid coated CdSe/ZnS QDs cytotoxicity in plant cells is dependent upon a number of factors including QDs properties, dose and the environmental conditions of administration and that, for <it>Medicago sativa </it>cells, a safe range of 1-5 nM should not be exceeded for biological applications.</p>
url http://www.jnanobiotechnology.com/content/8/1/24
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