Coupling an Electroactive <i>Pseudomonas Putida</i> KT2440 with Bioelectrochemical Rhamnolipid Production
Sufficient supply of oxygen is a major bottleneck in industrial biotechnological synthesis. One example is the heterologous production of rhamnolipids using <i>Pseudomonas putida</i> KT2440. Typically, the synthesis is accompanied by strong foam formation in the reactor vessel hampering...
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doaj-919318be1d3a4f30951f89608ad0d0ba2020-12-11T00:05:13ZengMDPI AGMicroorganisms2076-26072020-12-0181959195910.3390/microorganisms8121959Coupling an Electroactive <i>Pseudomonas Putida</i> KT2440 with Bioelectrochemical Rhamnolipid ProductionTheresia D. Askitosari0Carola Berger1Till Tiso2Falk Harnisch3Lars M. Blank4Miriam A. Rosenbaum5Institute of Applied Microbiology—iAMB, Aachen Biology and Biotechnology—ABBt, RWTH Aachen University, 52074 Aachen, GermanyLeibniz Institute for Natural Product Research and Infection Biology—Hans Knöll Institute, 07745 Jena, GermanyInstitute of Applied Microbiology—iAMB, Aachen Biology and Biotechnology—ABBt, RWTH Aachen University, 52074 Aachen, GermanyDepartment of Environmental Microbiology, Helmholtz Centre for Environmental Research—UFZ, 04318 Leipzig, GermanyInstitute of Applied Microbiology—iAMB, Aachen Biology and Biotechnology—ABBt, RWTH Aachen University, 52074 Aachen, GermanyLeibniz Institute for Natural Product Research and Infection Biology—Hans Knöll Institute, 07745 Jena, GermanySufficient supply of oxygen is a major bottleneck in industrial biotechnological synthesis. One example is the heterologous production of rhamnolipids using <i>Pseudomonas putida</i> KT2440. Typically, the synthesis is accompanied by strong foam formation in the reactor vessel hampering the process. It is caused by the extensive bubbling needed to sustain the high respirative oxygen demand in the presence of the produced surfactants. One way to reduce the oxygen requirement is to enable the cells to use the anode of a bioelectrochemical system (BES) as an alternative sink for their metabolically derived electrons. We here used a <i>P. putida</i> KT2440 strain that interacts with the anode using mediated extracellular electron transfer via intrinsically produced phenazines, to perform heterologous rhamnolipid production under oxygen limitation. The strain <i>P. putida</i> RL-PCA successfully produced 30.4 ± 4.7 mg/L mono-rhamnolipids together with 11.2 ± 0.8 mg/L of phenazine-1-carboxylic acid (PCA) in 500-mL benchtop BES reactors and 30.5 ± 0.5 mg/L rhamnolipids accompanied by 25.7 ± 8.0 mg/L PCA in electrode containing standard 1-L bioreactors. Hence, this study marks a first proof of concept to produce glycolipid surfactants in oxygen-limited BES with an industrially relevant strain.https://www.mdpi.com/2076-2607/8/12/1959<i>Pseudomonas putida</i>rhamnolipidbioelectrochemical systemphenazinesredox mediatormicrobial electrosynthesis |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Theresia D. Askitosari Carola Berger Till Tiso Falk Harnisch Lars M. Blank Miriam A. Rosenbaum |
spellingShingle |
Theresia D. Askitosari Carola Berger Till Tiso Falk Harnisch Lars M. Blank Miriam A. Rosenbaum Coupling an Electroactive <i>Pseudomonas Putida</i> KT2440 with Bioelectrochemical Rhamnolipid Production Microorganisms <i>Pseudomonas putida</i> rhamnolipid bioelectrochemical system phenazines redox mediator microbial electrosynthesis |
author_facet |
Theresia D. Askitosari Carola Berger Till Tiso Falk Harnisch Lars M. Blank Miriam A. Rosenbaum |
author_sort |
Theresia D. Askitosari |
title |
Coupling an Electroactive <i>Pseudomonas Putida</i> KT2440 with Bioelectrochemical Rhamnolipid Production |
title_short |
Coupling an Electroactive <i>Pseudomonas Putida</i> KT2440 with Bioelectrochemical Rhamnolipid Production |
title_full |
Coupling an Electroactive <i>Pseudomonas Putida</i> KT2440 with Bioelectrochemical Rhamnolipid Production |
title_fullStr |
Coupling an Electroactive <i>Pseudomonas Putida</i> KT2440 with Bioelectrochemical Rhamnolipid Production |
title_full_unstemmed |
Coupling an Electroactive <i>Pseudomonas Putida</i> KT2440 with Bioelectrochemical Rhamnolipid Production |
title_sort |
coupling an electroactive <i>pseudomonas putida</i> kt2440 with bioelectrochemical rhamnolipid production |
publisher |
MDPI AG |
series |
Microorganisms |
issn |
2076-2607 |
publishDate |
2020-12-01 |
description |
Sufficient supply of oxygen is a major bottleneck in industrial biotechnological synthesis. One example is the heterologous production of rhamnolipids using <i>Pseudomonas putida</i> KT2440. Typically, the synthesis is accompanied by strong foam formation in the reactor vessel hampering the process. It is caused by the extensive bubbling needed to sustain the high respirative oxygen demand in the presence of the produced surfactants. One way to reduce the oxygen requirement is to enable the cells to use the anode of a bioelectrochemical system (BES) as an alternative sink for their metabolically derived electrons. We here used a <i>P. putida</i> KT2440 strain that interacts with the anode using mediated extracellular electron transfer via intrinsically produced phenazines, to perform heterologous rhamnolipid production under oxygen limitation. The strain <i>P. putida</i> RL-PCA successfully produced 30.4 ± 4.7 mg/L mono-rhamnolipids together with 11.2 ± 0.8 mg/L of phenazine-1-carboxylic acid (PCA) in 500-mL benchtop BES reactors and 30.5 ± 0.5 mg/L rhamnolipids accompanied by 25.7 ± 8.0 mg/L PCA in electrode containing standard 1-L bioreactors. Hence, this study marks a first proof of concept to produce glycolipid surfactants in oxygen-limited BES with an industrially relevant strain. |
topic |
<i>Pseudomonas putida</i> rhamnolipid bioelectrochemical system phenazines redox mediator microbial electrosynthesis |
url |
https://www.mdpi.com/2076-2607/8/12/1959 |
work_keys_str_mv |
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