Hyperspectral Reflectance and Fluorescence Imaging to Detect Scab Induced Stress in Apple Leaves
Apple scab causes significant losses in the production of this fruit. A timely and more site-specific monitoring and spraying of the disease could reduce the number of applications of fungicides in the fruit industry. The aim of this leaf-scale study therefore lies in the early detection of apple sc...
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MDPI AG
2009-11-01
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Online Access: | http://www.mdpi.com/2072-4292/1/4/858/ |
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doaj-919631ae902749519cd6f1b87d0b81e02020-11-25T00:38:19ZengMDPI AGRemote Sensing2072-42922009-11-011485887410.3390/rs1040858Hyperspectral Reflectance and Fluorescence Imaging to Detect Scab Induced Stress in Apple LeavesJohan KeulemansStefaan LhermitteRoland ValckeBen SomersWillem W. VerstraetenAnnemarie AuwerkerkenStephanie DelalieuxPol CoppinApple scab causes significant losses in the production of this fruit. A timely and more site-specific monitoring and spraying of the disease could reduce the number of applications of fungicides in the fruit industry. The aim of this leaf-scale study therefore lies in the early detection of apple scab infections in a non-invasive and non-destructive way. In order to attain this objective, fluorescence- and hyperspectral imaging techniques were used. An experiment was conducted under controlled environmental conditions, linking hyperspectral reflectance and fluorescence imaging measurements to scab infection symptoms in a susceptible apple cultivar (Malus x domestica Borkh. cv. Braeburn). Plant stress was induced by inoculation of the apple plants with scab spores. The quantum efficiency of Photosystem II (PSII) photochemistry was derived from fluorescence images of leaves under light adapted conditions. Leaves inoculated with scab spores were expected to have lower PSII quantum efficiency than control (mock) leaves. However, besides scab-induced, also immature leaves exhibited low PSII quantum efficiency. Therefore, this study recommends the simultaneous use of fluorescence imaging and hyperspectral techniques. A shortwave infrared narrow-waveband ratio index (R1480/R2135) is presented in this paper as a promising tool to identify scab stress before symptoms become visible to the naked eye. Low PSII quantum efficiency attended by low narrow waveband R1480/R2135 index values points out scab stress in an early stage. Apparent high PSII quantum efficiency together with high overall reflectance in VIS and SWIR spectral domains indicate a severe, well-developed scab infection. http://www.mdpi.com/2072-4292/1/4/858/hyperspectral and fluorescence datascab detection |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Johan Keulemans Stefaan Lhermitte Roland Valcke Ben Somers Willem W. Verstraeten Annemarie Auwerkerken Stephanie Delalieux Pol Coppin |
spellingShingle |
Johan Keulemans Stefaan Lhermitte Roland Valcke Ben Somers Willem W. Verstraeten Annemarie Auwerkerken Stephanie Delalieux Pol Coppin Hyperspectral Reflectance and Fluorescence Imaging to Detect Scab Induced Stress in Apple Leaves Remote Sensing hyperspectral and fluorescence data scab detection |
author_facet |
Johan Keulemans Stefaan Lhermitte Roland Valcke Ben Somers Willem W. Verstraeten Annemarie Auwerkerken Stephanie Delalieux Pol Coppin |
author_sort |
Johan Keulemans |
title |
Hyperspectral Reflectance and Fluorescence Imaging to Detect Scab Induced Stress in Apple Leaves |
title_short |
Hyperspectral Reflectance and Fluorescence Imaging to Detect Scab Induced Stress in Apple Leaves |
title_full |
Hyperspectral Reflectance and Fluorescence Imaging to Detect Scab Induced Stress in Apple Leaves |
title_fullStr |
Hyperspectral Reflectance and Fluorescence Imaging to Detect Scab Induced Stress in Apple Leaves |
title_full_unstemmed |
Hyperspectral Reflectance and Fluorescence Imaging to Detect Scab Induced Stress in Apple Leaves |
title_sort |
hyperspectral reflectance and fluorescence imaging to detect scab induced stress in apple leaves |
publisher |
MDPI AG |
series |
Remote Sensing |
issn |
2072-4292 |
publishDate |
2009-11-01 |
description |
Apple scab causes significant losses in the production of this fruit. A timely and more site-specific monitoring and spraying of the disease could reduce the number of applications of fungicides in the fruit industry. The aim of this leaf-scale study therefore lies in the early detection of apple scab infections in a non-invasive and non-destructive way. In order to attain this objective, fluorescence- and hyperspectral imaging techniques were used. An experiment was conducted under controlled environmental conditions, linking hyperspectral reflectance and fluorescence imaging measurements to scab infection symptoms in a susceptible apple cultivar (Malus x domestica Borkh. cv. Braeburn). Plant stress was induced by inoculation of the apple plants with scab spores. The quantum efficiency of Photosystem II (PSII) photochemistry was derived from fluorescence images of leaves under light adapted conditions. Leaves inoculated with scab spores were expected to have lower PSII quantum efficiency than control (mock) leaves. However, besides scab-induced, also immature leaves exhibited low PSII quantum efficiency. Therefore, this study recommends the simultaneous use of fluorescence imaging and hyperspectral techniques. A shortwave infrared narrow-waveband ratio index (R1480/R2135) is presented in this paper as a promising tool to identify scab stress before symptoms become visible to the naked eye. Low PSII quantum efficiency attended by low narrow waveband R1480/R2135 index values points out scab stress in an early stage. Apparent high PSII quantum efficiency together with high overall reflectance in VIS and SWIR spectral domains indicate a severe, well-developed scab infection. |
topic |
hyperspectral and fluorescence data scab detection |
url |
http://www.mdpi.com/2072-4292/1/4/858/ |
work_keys_str_mv |
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