Development of a PVY Resistant Flue-Cured Tobacco Line via EMS Mutagenesis of <i>eIF4E</i>

• Main Authors: Lu Zhao, Wenzheng Li, Bingwu Wang, Yulong Gao, Xueyi Sui, Yong Liu, Xuejun Chen, Xuefeng Yao, Fangchan Jiao, Zhongbang Song
• Format: Article
• Language: English
• Published: MDPI AG 2019-12-01
• Series: Agronomy
• Subjects: <i>eif4e1-s</i>; potyvirus strains; <i>nicotiana tabacum</i> cv. yunyan87; ems-induced nonsense mutation; tilling; dcaps markers
• Online Access: https://www.mdpi.com/2073-4395/10/1/36

Recessive resistance against potyviruses, such as <i>Potato virus Y</i> (PVY), relies on mutations in the eukaryotic translation initiation factor 4E (eIF4E) or one of its isoforms. The <i>eIF4E1-S</i> mutants of burley tobacco (<i>Nicotiana tabacum</i> L.) exhibit recessive resistance against PVY strains. Here, we developed a TILLING population of flue-cured tobacco (<i>N. tabacum</i> cv. Yunyan87) using ethyl methanesulfonate (EMS) to identify <i>eIF4E1-S</i> mutants. M3 plants homozygous for a nonsense mutation in exon 1 of the <i>eIF4E1-S</i> gene demonstrated resistance against PVY^MN. These M3 plants were backcrossed to &#8216;Yunyan87&#8217;, and BC₄F₃ plants were screened using derived cleaved amplified polymorphic sequence (dCAPS) markers. BC₄F₃ plants showing agronomic traits comparable to the recurrent parent &#8216;Yunyan87&#8217; and resistance against PVY^O, PVY^N, and PVY^NTN strains were identified. These genotypes would provide useful germplasm for future tobacco improvement and would aid in basic research on PVY resistance in flue-cured tobacco.