Gene expression profiling and pathway analysis data in MCF-7 and MDA-MB-231 human breast cancer cell lines treated with dioscin
Microarray technology (Human OneArray microarray, phylanxbiotech.com) was used to compare gene expression profiles of non-invasive MCF-7 and invasive MDA-MB-231 breast cancer cells exposed to dioscin (DS), a steroidal saponin isolated from the roots of wild yam, (Dioscorea villosa). Initially the di...
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doaj-91afc1873c584ca9a49e346a537079872020-11-25T00:47:49ZengElsevierData in Brief2352-34092016-09-018272279Gene expression profiling and pathway analysis data in MCF-7 and MDA-MB-231 human breast cancer cell lines treated with dioscinPranapda Aumsuwan0Shabana I. Khan1Ikhlas A. Khan2Larry A. Walker3Asok K. Dasmahapatra4National Center for Natural Product Research, University of Mississippi, MS 38677, USA; Division of Pharmacology, Department of BioMolecular Sciences, University of Mississippi, MS 38677, USANational Center for Natural Product Research, University of Mississippi, MS 38677, USA; Division of Pharmacognosy, Department of BioMolecular sciences, School of Pharmacy, University of Mississippi, MS 38677, USANational Center for Natural Product Research, University of Mississippi, MS 38677, USA; Division of Pharmacognosy, Department of BioMolecular sciences, School of Pharmacy, University of Mississippi, MS 38677, USANational Center for Natural Product Research, University of Mississippi, MS 38677, USA; Division of Pharmacology, Department of BioMolecular Sciences, University of Mississippi, MS 38677, USA; University of Mississippi Cancer Institute (Oxford Campus), School of Pharmacy, University of Mississippi, MS 38677, USANational Center for Natural Product Research, University of Mississippi, MS 38677, USA; Division of Pharmacology, Department of BioMolecular Sciences, University of Mississippi, MS 38677, USA; University of Mississippi Cancer Institute (Oxford Campus), School of Pharmacy, University of Mississippi, MS 38677, USA; Corresponding author at: National Center for Natural Product Research Department of BioMolecular Sciences Pharmacology Division 313 Faser Hall University MS 38677 USA. Tel.: +1 662 915 7077; fax: +1 662 915 5148.Microarray technology (Human OneArray microarray, phylanxbiotech.com) was used to compare gene expression profiles of non-invasive MCF-7 and invasive MDA-MB-231 breast cancer cells exposed to dioscin (DS), a steroidal saponin isolated from the roots of wild yam, (Dioscorea villosa). Initially the differential expression of genes (DEG) was identified which was followed by pathway enrichment analysis (PEA). Of the genes queried on OneArray, we identified 4641 DEG changed between MCF-7 and MDA-MB-231 cells (vehicle-treated) with cut-off log2 |fold change|≧1. Among these genes, 2439 genes were upregulated and 2002 were downregulated. DS exposure (2.30 μM, 72 h) to these cells identified 801 (MCF-7) and 96 (MDA-MB-231) DEG that showed significant difference when compared with the untreated cells (p<0.05). Within these gene sets, DS was able to upregulate 395 genes and downregulate 406 genes in MCF-7 and upregulate 36 and downregulate 60 genes in MDA-MB-231 cells. Further comparison of DEG between MCF-7 and MDA-MB-231 cells exposed to DS identified 3626 DEG of which 1700 were upregulated and 1926 were down-regulated. Regarding to PEA, 12 canonical pathways were significantly altered between these two cell lines. However, there was no alteration in any of these pathways in MCF-7 cells, while in MDA-MB-231 cells only MAPK pathway showed significant alteration. When PEA comparison was made on DS exposed cells, it was observed that only 2 pathways were significantly affected. Further, we identified the shared DEG, which were targeted by DS and overlapped in both MCF-7 and MDA-MB-231 cells, by intersection analysis (Venn diagram). We found that 7 DEG were overlapped of which six are reported in the database. This data highlight the diverse gene networks and pathways in MCF-7 and MDA-MB-231 human breast cancer cell lines treated with dioscin.http://www.sciencedirect.com/science/article/pii/S2352340916303286 |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Pranapda Aumsuwan Shabana I. Khan Ikhlas A. Khan Larry A. Walker Asok K. Dasmahapatra |
spellingShingle |
Pranapda Aumsuwan Shabana I. Khan Ikhlas A. Khan Larry A. Walker Asok K. Dasmahapatra Gene expression profiling and pathway analysis data in MCF-7 and MDA-MB-231 human breast cancer cell lines treated with dioscin Data in Brief |
author_facet |
Pranapda Aumsuwan Shabana I. Khan Ikhlas A. Khan Larry A. Walker Asok K. Dasmahapatra |
author_sort |
Pranapda Aumsuwan |
title |
Gene expression profiling and pathway analysis data in MCF-7 and MDA-MB-231 human breast cancer cell lines treated with dioscin |
title_short |
Gene expression profiling and pathway analysis data in MCF-7 and MDA-MB-231 human breast cancer cell lines treated with dioscin |
title_full |
Gene expression profiling and pathway analysis data in MCF-7 and MDA-MB-231 human breast cancer cell lines treated with dioscin |
title_fullStr |
Gene expression profiling and pathway analysis data in MCF-7 and MDA-MB-231 human breast cancer cell lines treated with dioscin |
title_full_unstemmed |
Gene expression profiling and pathway analysis data in MCF-7 and MDA-MB-231 human breast cancer cell lines treated with dioscin |
title_sort |
gene expression profiling and pathway analysis data in mcf-7 and mda-mb-231 human breast cancer cell lines treated with dioscin |
publisher |
Elsevier |
series |
Data in Brief |
issn |
2352-3409 |
publishDate |
2016-09-01 |
description |
Microarray technology (Human OneArray microarray, phylanxbiotech.com) was used to compare gene expression profiles of non-invasive MCF-7 and invasive MDA-MB-231 breast cancer cells exposed to dioscin (DS), a steroidal saponin isolated from the roots of wild yam, (Dioscorea villosa). Initially the differential expression of genes (DEG) was identified which was followed by pathway enrichment analysis (PEA). Of the genes queried on OneArray, we identified 4641 DEG changed between MCF-7 and MDA-MB-231 cells (vehicle-treated) with cut-off log2 |fold change|≧1. Among these genes, 2439 genes were upregulated and 2002 were downregulated. DS exposure (2.30 μM, 72 h) to these cells identified 801 (MCF-7) and 96 (MDA-MB-231) DEG that showed significant difference when compared with the untreated cells (p<0.05). Within these gene sets, DS was able to upregulate 395 genes and downregulate 406 genes in MCF-7 and upregulate 36 and downregulate 60 genes in MDA-MB-231 cells. Further comparison of DEG between MCF-7 and MDA-MB-231 cells exposed to DS identified 3626 DEG of which 1700 were upregulated and 1926 were down-regulated. Regarding to PEA, 12 canonical pathways were significantly altered between these two cell lines. However, there was no alteration in any of these pathways in MCF-7 cells, while in MDA-MB-231 cells only MAPK pathway showed significant alteration. When PEA comparison was made on DS exposed cells, it was observed that only 2 pathways were significantly affected. Further, we identified the shared DEG, which were targeted by DS and overlapped in both MCF-7 and MDA-MB-231 cells, by intersection analysis (Venn diagram). We found that 7 DEG were overlapped of which six are reported in the database. This data highlight the diverse gene networks and pathways in MCF-7 and MDA-MB-231 human breast cancer cell lines treated with dioscin. |
url |
http://www.sciencedirect.com/science/article/pii/S2352340916303286 |
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