Synergistic Activities of Abdominal Muscles Are Required for Efficient Micturition in Anesthetized Female Mice
Purpose To characterize the electromyographic activity of abdominal striated muscles during micturition in urethane-anesthetized female mice, and to quantitatively evaluate the contribution of abdominal responses to efficient voiding. Methods Cystometric and multichannel electromyographic recordings...
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Korean Continence Society
2018-03-01
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doaj-91b76df7ebb34f21a4356dc45a57d44e2020-11-24T23:02:55ZengKorean Continence SocietyInternational Neurourology Journal2093-47772093-69312018-03-0122191910.5213/inj.1835052.526717Synergistic Activities of Abdominal Muscles Are Required for Efficient Micturition in Anesthetized Female MiceChuan Zhang0Yingchun Zhang1Yolanda Cruz2Timothy B. Boone3Alvaro Munoz4 Department of Biomedical Engineering, University of Houston, Houston, TX, USA Department of Biomedical Engineering, University of Houston, Houston, TX, USA Centro Tlaxcala de Biología de la Conducta, Universidad Autónoma de Tlaxcala, Tlaxcala, México Regenerative Medicine and Tissue Engineering Program-Urology, Houston Methodist Research Institute, Houston, TX, USA Regenerative Medicine and Tissue Engineering Program-Urology, Houston Methodist Research Institute, Houston, TX, USAPurpose To characterize the electromyographic activity of abdominal striated muscles during micturition in urethane-anesthetized female mice, and to quantitatively evaluate the contribution of abdominal responses to efficient voiding. Methods Cystometric and multichannel electromyographic recordings were integrated to enable a comprehensive evaluation during micturition in urethane-anesthetized female mice. Four major abdominal muscle domains were evaluated: the external oblique, internal oblique, and superior and inferior rectus abdominis. To further characterize the functionality of the abdominal muscles, pancuronium bromide (25 μg/mL or 50 μg/mL, abdominal surface) was applied as a blocking agent of neuromuscular junctions. Results We observed a robust activation of the abdominal muscles during voiding, with a consistent onset/offset concomitant with the bladder pressure threshold. Pancuronium was effective, in a dose-dependent fashion, for partial and complete blockage of abdominal activity. Electromyographic discharges during voiding were significantly inhibited by applying pancuronium. Decreased cystometric parameters were recorded, including the peak pressure, pressure threshold, intercontractile interval, and voiding duration, suggesting that the voiding efficiency was significantly compromised by abdominal muscle relaxation. Conclusions The relevance of the abdominal striated musculature for micturition has remained a topic of debate in human physiology. Although the study was performed on anesthetized mice, these results support the existence of synergistic abdominal electromyographic activity facilitating voiding in anesthetized mice. Further, our study presents a rodent model that can be used for future investigations into micturition-related abdominal activity.http://www.einj.org/upload/pdf/inj-1835052-526.pdfLower urinary tract symptomsMicturitionAbdominal musclesElectromyographyCystometry |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Chuan Zhang Yingchun Zhang Yolanda Cruz Timothy B. Boone Alvaro Munoz |
spellingShingle |
Chuan Zhang Yingchun Zhang Yolanda Cruz Timothy B. Boone Alvaro Munoz Synergistic Activities of Abdominal Muscles Are Required for Efficient Micturition in Anesthetized Female Mice International Neurourology Journal Lower urinary tract symptoms Micturition Abdominal muscles Electromyography Cystometry |
author_facet |
Chuan Zhang Yingchun Zhang Yolanda Cruz Timothy B. Boone Alvaro Munoz |
author_sort |
Chuan Zhang |
title |
Synergistic Activities of Abdominal Muscles Are Required for Efficient Micturition in Anesthetized Female Mice |
title_short |
Synergistic Activities of Abdominal Muscles Are Required for Efficient Micturition in Anesthetized Female Mice |
title_full |
Synergistic Activities of Abdominal Muscles Are Required for Efficient Micturition in Anesthetized Female Mice |
title_fullStr |
Synergistic Activities of Abdominal Muscles Are Required for Efficient Micturition in Anesthetized Female Mice |
title_full_unstemmed |
Synergistic Activities of Abdominal Muscles Are Required for Efficient Micturition in Anesthetized Female Mice |
title_sort |
synergistic activities of abdominal muscles are required for efficient micturition in anesthetized female mice |
publisher |
Korean Continence Society |
series |
International Neurourology Journal |
issn |
2093-4777 2093-6931 |
publishDate |
2018-03-01 |
description |
Purpose To characterize the electromyographic activity of abdominal striated muscles during micturition in urethane-anesthetized female mice, and to quantitatively evaluate the contribution of abdominal responses to efficient voiding. Methods Cystometric and multichannel electromyographic recordings were integrated to enable a comprehensive evaluation during micturition in urethane-anesthetized female mice. Four major abdominal muscle domains were evaluated: the external oblique, internal oblique, and superior and inferior rectus abdominis. To further characterize the functionality of the abdominal muscles, pancuronium bromide (25 μg/mL or 50 μg/mL, abdominal surface) was applied as a blocking agent of neuromuscular junctions. Results We observed a robust activation of the abdominal muscles during voiding, with a consistent onset/offset concomitant with the bladder pressure threshold. Pancuronium was effective, in a dose-dependent fashion, for partial and complete blockage of abdominal activity. Electromyographic discharges during voiding were significantly inhibited by applying pancuronium. Decreased cystometric parameters were recorded, including the peak pressure, pressure threshold, intercontractile interval, and voiding duration, suggesting that the voiding efficiency was significantly compromised by abdominal muscle relaxation. Conclusions The relevance of the abdominal striated musculature for micturition has remained a topic of debate in human physiology. Although the study was performed on anesthetized mice, these results support the existence of synergistic abdominal electromyographic activity facilitating voiding in anesthetized mice. Further, our study presents a rodent model that can be used for future investigations into micturition-related abdominal activity. |
topic |
Lower urinary tract symptoms Micturition Abdominal muscles Electromyography Cystometry |
url |
http://www.einj.org/upload/pdf/inj-1835052-526.pdf |
work_keys_str_mv |
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