Transcriptional Variation in Glucosinolate Biosynthetic Genes and Inducible Responses to Aphid Herbivory on Field-Grown Arabidopsis thaliana
Recently, increasing attempts have been made to understand how plant genes function in natura. In this context, transcriptional profiles represent plant physiological status in response to environmental stimuli. Herein, we combined high-throughput RNA-Seq with insect survey data on 19 accessions of...
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doaj-91c7d37fe04148dfae42930a4d0453302020-11-25T00:53:23ZengFrontiers Media S.A.Frontiers in Genetics1664-80212019-09-011010.3389/fgene.2019.00787469835Transcriptional Variation in Glucosinolate Biosynthetic Genes and Inducible Responses to Aphid Herbivory on Field-Grown Arabidopsis thalianaYasuhiro Sato0Yasuhiro Sato1Ayumi Tezuka2Makoto Kashima3Ayumi Deguchi4Ayumi Deguchi5Rie Shimizu-Inatsugi6Misako Yamazaki7Kentaro K. Shimizu8Kentaro K. Shimizu9Atsushi J. Nagano10PRESTO, Japan Science and Technology Agency, Kawaguchi, JapanResearch Institute for Food and Agriculture, Ryukoku University, Otsu, JapanResearch Institute for Food and Agriculture, Ryukoku University, Otsu, JapanResearch Institute for Food and Agriculture, Ryukoku University, Otsu, JapanResearch Institute for Food and Agriculture, Ryukoku University, Otsu, JapanGraduate School of Horticulture, Chiba University, Matsudo, JapanDepartment of Evolutionary Biology and Environmental Studies, University of Zurich, Zurich, SwitzerlandDepartment of Evolutionary Biology and Environmental Studies, University of Zurich, Zurich, SwitzerlandDepartment of Evolutionary Biology and Environmental Studies, University of Zurich, Zurich, SwitzerlandKihara Institute for Biological Research, Yokohama City University, Yokohama, JapanDepartment of Plant Life Sciences, Faculty of Agriculture, Ryukoku University, Otsu, JapanRecently, increasing attempts have been made to understand how plant genes function in natura. In this context, transcriptional profiles represent plant physiological status in response to environmental stimuli. Herein, we combined high-throughput RNA-Seq with insect survey data on 19 accessions of Arabidopsis thaliana grown at a field site in Switzerland. We found that genes with the gene ontology (GO) annotations of “glucosinolate biosynthetic process” and “response to insects” were most significantly enriched, and the expression of these genes was highly variable among plant accessions. Nearly half of the total expression variation in the glucosinolate biosynthetic genes (AOPs, ESM1, ESP, and TGG1) was explained by among-accession variation. Of these genes, the expression level of AOP3 differed among Col-0 accession individuals depending on the abundance of the mustard aphid (Lipaphis erysimi). We also found that the expression of the major cis-jasmone activated gene CYP81D11 was positively correlated with the number of flea beetles (Phyllotreta striolata and Phyllotreta atra). Combined with the field RNA-Seq data, bioassays confirmed that AOP3 was up-regulated in response to attack by mustard aphids. The combined results from RNA-Seq and our ecological survey illustrate the feasibility of using field transcriptomics to detect an inducible defense, providing a first step towards an in natura understanding of biotic interactions involving phenotypic plasticity.https://www.frontiersin.org/article/10.3389/fgene.2019.00787/fullAOP3in naturaLipaphis erysimiRNA-Seqplant–insect interaction |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Yasuhiro Sato Yasuhiro Sato Ayumi Tezuka Makoto Kashima Ayumi Deguchi Ayumi Deguchi Rie Shimizu-Inatsugi Misako Yamazaki Kentaro K. Shimizu Kentaro K. Shimizu Atsushi J. Nagano |
spellingShingle |
Yasuhiro Sato Yasuhiro Sato Ayumi Tezuka Makoto Kashima Ayumi Deguchi Ayumi Deguchi Rie Shimizu-Inatsugi Misako Yamazaki Kentaro K. Shimizu Kentaro K. Shimizu Atsushi J. Nagano Transcriptional Variation in Glucosinolate Biosynthetic Genes and Inducible Responses to Aphid Herbivory on Field-Grown Arabidopsis thaliana Frontiers in Genetics AOP3 in natura Lipaphis erysimi RNA-Seq plant–insect interaction |
author_facet |
Yasuhiro Sato Yasuhiro Sato Ayumi Tezuka Makoto Kashima Ayumi Deguchi Ayumi Deguchi Rie Shimizu-Inatsugi Misako Yamazaki Kentaro K. Shimizu Kentaro K. Shimizu Atsushi J. Nagano |
author_sort |
Yasuhiro Sato |
title |
Transcriptional Variation in Glucosinolate Biosynthetic Genes and Inducible Responses to Aphid Herbivory on Field-Grown Arabidopsis thaliana |
title_short |
Transcriptional Variation in Glucosinolate Biosynthetic Genes and Inducible Responses to Aphid Herbivory on Field-Grown Arabidopsis thaliana |
title_full |
Transcriptional Variation in Glucosinolate Biosynthetic Genes and Inducible Responses to Aphid Herbivory on Field-Grown Arabidopsis thaliana |
title_fullStr |
Transcriptional Variation in Glucosinolate Biosynthetic Genes and Inducible Responses to Aphid Herbivory on Field-Grown Arabidopsis thaliana |
title_full_unstemmed |
Transcriptional Variation in Glucosinolate Biosynthetic Genes and Inducible Responses to Aphid Herbivory on Field-Grown Arabidopsis thaliana |
title_sort |
transcriptional variation in glucosinolate biosynthetic genes and inducible responses to aphid herbivory on field-grown arabidopsis thaliana |
publisher |
Frontiers Media S.A. |
series |
Frontiers in Genetics |
issn |
1664-8021 |
publishDate |
2019-09-01 |
description |
Recently, increasing attempts have been made to understand how plant genes function in natura. In this context, transcriptional profiles represent plant physiological status in response to environmental stimuli. Herein, we combined high-throughput RNA-Seq with insect survey data on 19 accessions of Arabidopsis thaliana grown at a field site in Switzerland. We found that genes with the gene ontology (GO) annotations of “glucosinolate biosynthetic process” and “response to insects” were most significantly enriched, and the expression of these genes was highly variable among plant accessions. Nearly half of the total expression variation in the glucosinolate biosynthetic genes (AOPs, ESM1, ESP, and TGG1) was explained by among-accession variation. Of these genes, the expression level of AOP3 differed among Col-0 accession individuals depending on the abundance of the mustard aphid (Lipaphis erysimi). We also found that the expression of the major cis-jasmone activated gene CYP81D11 was positively correlated with the number of flea beetles (Phyllotreta striolata and Phyllotreta atra). Combined with the field RNA-Seq data, bioassays confirmed that AOP3 was up-regulated in response to attack by mustard aphids. The combined results from RNA-Seq and our ecological survey illustrate the feasibility of using field transcriptomics to detect an inducible defense, providing a first step towards an in natura understanding of biotic interactions involving phenotypic plasticity. |
topic |
AOP3 in natura Lipaphis erysimi RNA-Seq plant–insect interaction |
url |
https://www.frontiersin.org/article/10.3389/fgene.2019.00787/full |
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