The Cellular Origins of Chick Global Flash Multifocal Electroretinogram
Purpose: The aim of this study was to obtain a better understanding of the cellular contributions to the chick global flash mfERG by using a pharmacological dissection method. Method: Global flash mfERGs were recorded from 11 white leghorn chicks (Gallus gallus). The inner retinal response was suppr...
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doaj-91d3dff7917e416b95bcfc0d0110d5ad2020-11-25T03:09:18ZengSAGE Publishingi-Perception2041-66952011-05-01210.1068/ic30410.1068_ic304The Cellular Origins of Chick Global Flash Multifocal ElectroretinogramPatrick W. K. Ting0Patrick H. W. Chu1Yf Ng2Henry H. L. Chan3Laboratory of Experimental Optometry (Neuroscience), School of Optometry, The Hong Kong Polytechnic University, Hung Hom, Kowloon, Hong Kong.Laboratory of Experimental Optometry (Neuroscience), School of Optometry, The Hong Kong Polytechnic University, Hung Hom, Kowloon, Hong Kong.Laboratory of Experimental Optometry (Neuroscience), School of Optometry, The Hong Kong Polytechnic University, Hung Hom, Kowloon, Hong Kong.Laboratory of Experimental Optometry (Neuroscience), School of Optometry, The Hong Kong Polytechnic University, Hung Hom, Kowloon, Hong Kong.Purpose: The aim of this study was to obtain a better understanding of the cellular contributions to the chick global flash mfERG by using a pharmacological dissection method. Method: Global flash mfERGs were recorded from 11 white leghorn chicks (Gallus gallus). The inner retinal response was suppressed by injection of tetrodotoxin (TTX) and N-methyl-D-aspartic acid (NMDA). Responses from ON- and OFF-pathway were isolated by further injection of 2-amino-4-phosphonobutyric acid (APB). Results: The global flash mfERG of white leghorn chicks consist of a direct component (DC) originated from outer retina and a late induced component (IC) originated from the inner retina which are comparable to the global flash mfERG responses from primate, porcine and human. The ON- and OFF- responses found in chicks are also similar to that of porcine and primate with the N1 component is mainly from OFF-bipolar responses and the ON-response contributes mainly in the P1 component. Conclusion: Because of the similarities for the global flash mfERG found in chick to other species, this makes the possibility of using chick retina as a potential animal model for eye research in the area of mfERG.https://doi.org/10.1068/ic304 |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Patrick W. K. Ting Patrick H. W. Chu Yf Ng Henry H. L. Chan |
spellingShingle |
Patrick W. K. Ting Patrick H. W. Chu Yf Ng Henry H. L. Chan The Cellular Origins of Chick Global Flash Multifocal Electroretinogram i-Perception |
author_facet |
Patrick W. K. Ting Patrick H. W. Chu Yf Ng Henry H. L. Chan |
author_sort |
Patrick W. K. Ting |
title |
The Cellular Origins of Chick Global Flash Multifocal Electroretinogram |
title_short |
The Cellular Origins of Chick Global Flash Multifocal Electroretinogram |
title_full |
The Cellular Origins of Chick Global Flash Multifocal Electroretinogram |
title_fullStr |
The Cellular Origins of Chick Global Flash Multifocal Electroretinogram |
title_full_unstemmed |
The Cellular Origins of Chick Global Flash Multifocal Electroretinogram |
title_sort |
cellular origins of chick global flash multifocal electroretinogram |
publisher |
SAGE Publishing |
series |
i-Perception |
issn |
2041-6695 |
publishDate |
2011-05-01 |
description |
Purpose: The aim of this study was to obtain a better understanding of the cellular contributions to the chick global flash mfERG by using a pharmacological dissection method. Method: Global flash mfERGs were recorded from 11 white leghorn chicks (Gallus gallus). The inner retinal response was suppressed by injection of tetrodotoxin (TTX) and N-methyl-D-aspartic acid (NMDA). Responses from ON- and OFF-pathway were isolated by further injection of 2-amino-4-phosphonobutyric acid (APB). Results: The global flash mfERG of white leghorn chicks consist of a direct component (DC) originated from outer retina and a late induced component (IC) originated from the inner retina which are comparable to the global flash mfERG responses from primate, porcine and human. The ON- and OFF- responses found in chicks are also similar to that of porcine and primate with the N1 component is mainly from OFF-bipolar responses and the ON-response contributes mainly in the P1 component. Conclusion: Because of the similarities for the global flash mfERG found in chick to other species, this makes the possibility of using chick retina as a potential animal model for eye research in the area of mfERG. |
url |
https://doi.org/10.1068/ic304 |
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