Production of Ethylene Glycol from Glycerol Using an In Vitro Enzymatic Cascade
Glycerol is a readily available and inexpensive substance that is mostly generated during biofuel production processes. In order to ensure the viability of the biofuel industry, it is essential to develop complementing technologies for the resource utilization of glycerol. Ethylene glycol is a two-c...
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doaj-91d51c2549174e3ba2c56e856fdaffc92021-02-06T00:04:37ZengMDPI AGCatalysts2073-43442021-02-011121421410.3390/catal11020214Production of Ethylene Glycol from Glycerol Using an In Vitro Enzymatic CascadeKai Li0Weikang Sun1Wensi Meng2Jinxin Yan3Yipeng Zhang4Shiting Guo5Chuanjuan Lü6Cuiqing Ma7Chao Gao8State Key Laboratory of Microbial Technology, Shandong University, Qingdao 266237, ChinaState Key Laboratory of Microbial Technology, Shandong University, Qingdao 266237, ChinaState Key Laboratory of Microbial Technology, Shandong University, Qingdao 266237, ChinaState Key Laboratory of Microbial Technology, Shandong University, Qingdao 266237, ChinaState Key Laboratory of Microbial Technology, Shandong University, Qingdao 266237, ChinaState Key Laboratory of Microbial Technology, Shandong University, Qingdao 266237, ChinaState Key Laboratory of Microbial Technology, Shandong University, Qingdao 266237, ChinaState Key Laboratory of Microbial Technology, Shandong University, Qingdao 266237, ChinaState Key Laboratory of Microbial Technology, Shandong University, Qingdao 266237, ChinaGlycerol is a readily available and inexpensive substance that is mostly generated during biofuel production processes. In order to ensure the viability of the biofuel industry, it is essential to develop complementing technologies for the resource utilization of glycerol. Ethylene glycol is a two-carbon organic chemical with multiple applications and a huge market. In this study, an artificial enzymatic cascade comprised alditol oxidase, catalase, glyoxylate/hydroxypyruvate reductase, pyruvate decarboxylase and lactaldehyde:propanediol oxidoreductase was developed for the production of ethylene glycol from glycerol. The reduced nicotinamide adenine dinucleotide (NADH) generated during the dehydrogenation of the glycerol oxidation product <span style="font-variant: small-caps;">d</span>-glycerate can be as the reductant to support the ethylene glycol production. Using this in vitro synthetic system with self-sufficient NADH recycling, 7.64 ± 0.15 mM ethylene glycol was produced from 10 mM glycerol in 10 h, with a high yield of 0.515 ± 0.1 g/g. The in vitro enzymatic cascade is not only a promising alternative for the generation of ethylene glycol but also a successful example of the value-added utilization of glycerol.https://www.mdpi.com/2073-4344/11/2/214glycerolethylene glycolbiocatalysisin vitro enzymatic cascade |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Kai Li Weikang Sun Wensi Meng Jinxin Yan Yipeng Zhang Shiting Guo Chuanjuan Lü Cuiqing Ma Chao Gao |
spellingShingle |
Kai Li Weikang Sun Wensi Meng Jinxin Yan Yipeng Zhang Shiting Guo Chuanjuan Lü Cuiqing Ma Chao Gao Production of Ethylene Glycol from Glycerol Using an In Vitro Enzymatic Cascade Catalysts glycerol ethylene glycol biocatalysis in vitro enzymatic cascade |
author_facet |
Kai Li Weikang Sun Wensi Meng Jinxin Yan Yipeng Zhang Shiting Guo Chuanjuan Lü Cuiqing Ma Chao Gao |
author_sort |
Kai Li |
title |
Production of Ethylene Glycol from Glycerol Using an In Vitro Enzymatic Cascade |
title_short |
Production of Ethylene Glycol from Glycerol Using an In Vitro Enzymatic Cascade |
title_full |
Production of Ethylene Glycol from Glycerol Using an In Vitro Enzymatic Cascade |
title_fullStr |
Production of Ethylene Glycol from Glycerol Using an In Vitro Enzymatic Cascade |
title_full_unstemmed |
Production of Ethylene Glycol from Glycerol Using an In Vitro Enzymatic Cascade |
title_sort |
production of ethylene glycol from glycerol using an in vitro enzymatic cascade |
publisher |
MDPI AG |
series |
Catalysts |
issn |
2073-4344 |
publishDate |
2021-02-01 |
description |
Glycerol is a readily available and inexpensive substance that is mostly generated during biofuel production processes. In order to ensure the viability of the biofuel industry, it is essential to develop complementing technologies for the resource utilization of glycerol. Ethylene glycol is a two-carbon organic chemical with multiple applications and a huge market. In this study, an artificial enzymatic cascade comprised alditol oxidase, catalase, glyoxylate/hydroxypyruvate reductase, pyruvate decarboxylase and lactaldehyde:propanediol oxidoreductase was developed for the production of ethylene glycol from glycerol. The reduced nicotinamide adenine dinucleotide (NADH) generated during the dehydrogenation of the glycerol oxidation product <span style="font-variant: small-caps;">d</span>-glycerate can be as the reductant to support the ethylene glycol production. Using this in vitro synthetic system with self-sufficient NADH recycling, 7.64 ± 0.15 mM ethylene glycol was produced from 10 mM glycerol in 10 h, with a high yield of 0.515 ± 0.1 g/g. The in vitro enzymatic cascade is not only a promising alternative for the generation of ethylene glycol but also a successful example of the value-added utilization of glycerol. |
topic |
glycerol ethylene glycol biocatalysis in vitro enzymatic cascade |
url |
https://www.mdpi.com/2073-4344/11/2/214 |
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