The Pseudomonas syringae pv. tomato DC3000 PSPTO_0820 multidrug transporter is involved in resistance to plant antimicrobials and bacterial survival during tomato plant infection.
Multidrug resistance efflux pumps protect bacterial cells against a wide spectrum of antimicrobial compounds. PSPTO_0820 is a predicted multidrug transporter from the phytopathogenic bacterium Pseudomonas syringae pv. tomato DC3000. Orthologs of this protein are conserved within many Pseudomonas spe...
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doaj-920462b17bd74187a2540ba454d7feea2021-03-03T20:36:40ZengPublic Library of Science (PLoS)PLoS ONE1932-62032019-01-01146e021881510.1371/journal.pone.0218815The Pseudomonas syringae pv. tomato DC3000 PSPTO_0820 multidrug transporter is involved in resistance to plant antimicrobials and bacterial survival during tomato plant infection.Saray Santamaría-HernandoMarta SenovillaAlmudena González-MulaPedro Manuel Martínez-GarcíaSandra NebredaPablo Rodríguez-PalenzuelaEmilia López-SolanillaJosé Juan Rodríguez-HervaMultidrug resistance efflux pumps protect bacterial cells against a wide spectrum of antimicrobial compounds. PSPTO_0820 is a predicted multidrug transporter from the phytopathogenic bacterium Pseudomonas syringae pv. tomato DC3000. Orthologs of this protein are conserved within many Pseudomonas species that interact with plants. To study the potential role of PSPTO_0820 in plant-bacteria interaction, a mutant in this gene was isolated and characterized. In addition, with the aim to find the outer membrane channel for this efflux system, a mutant in PSPTO_4977, a TolC-like gene, was also analyzed. Both mutants were more susceptible to trans-cinnamic and chlorogenic acids and to the flavonoid (+)-catechin, when added to the culture medium. The expression level of both genes increased in the presence of (+)-catechin and, in the case of PSPTO_0820, also in response to trans-cinnamic acid. PSPTO_0820 and PSPTO_4977 mutants were unable to colonize tomato at high population levels. This work evidences the involvement of these two proteins in the resistance to plant antimicrobials, supporting also the importance of chlorogenic acid, trans-cinnamic acid, and (+)-catechin in the tomato plant defense response against P. syringae pv. tomato DC3000 infection.https://doi.org/10.1371/journal.pone.0218815 |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Saray Santamaría-Hernando Marta Senovilla Almudena González-Mula Pedro Manuel Martínez-García Sandra Nebreda Pablo Rodríguez-Palenzuela Emilia López-Solanilla José Juan Rodríguez-Herva |
spellingShingle |
Saray Santamaría-Hernando Marta Senovilla Almudena González-Mula Pedro Manuel Martínez-García Sandra Nebreda Pablo Rodríguez-Palenzuela Emilia López-Solanilla José Juan Rodríguez-Herva The Pseudomonas syringae pv. tomato DC3000 PSPTO_0820 multidrug transporter is involved in resistance to plant antimicrobials and bacterial survival during tomato plant infection. PLoS ONE |
author_facet |
Saray Santamaría-Hernando Marta Senovilla Almudena González-Mula Pedro Manuel Martínez-García Sandra Nebreda Pablo Rodríguez-Palenzuela Emilia López-Solanilla José Juan Rodríguez-Herva |
author_sort |
Saray Santamaría-Hernando |
title |
The Pseudomonas syringae pv. tomato DC3000 PSPTO_0820 multidrug transporter is involved in resistance to plant antimicrobials and bacterial survival during tomato plant infection. |
title_short |
The Pseudomonas syringae pv. tomato DC3000 PSPTO_0820 multidrug transporter is involved in resistance to plant antimicrobials and bacterial survival during tomato plant infection. |
title_full |
The Pseudomonas syringae pv. tomato DC3000 PSPTO_0820 multidrug transporter is involved in resistance to plant antimicrobials and bacterial survival during tomato plant infection. |
title_fullStr |
The Pseudomonas syringae pv. tomato DC3000 PSPTO_0820 multidrug transporter is involved in resistance to plant antimicrobials and bacterial survival during tomato plant infection. |
title_full_unstemmed |
The Pseudomonas syringae pv. tomato DC3000 PSPTO_0820 multidrug transporter is involved in resistance to plant antimicrobials and bacterial survival during tomato plant infection. |
title_sort |
pseudomonas syringae pv. tomato dc3000 pspto_0820 multidrug transporter is involved in resistance to plant antimicrobials and bacterial survival during tomato plant infection. |
publisher |
Public Library of Science (PLoS) |
series |
PLoS ONE |
issn |
1932-6203 |
publishDate |
2019-01-01 |
description |
Multidrug resistance efflux pumps protect bacterial cells against a wide spectrum of antimicrobial compounds. PSPTO_0820 is a predicted multidrug transporter from the phytopathogenic bacterium Pseudomonas syringae pv. tomato DC3000. Orthologs of this protein are conserved within many Pseudomonas species that interact with plants. To study the potential role of PSPTO_0820 in plant-bacteria interaction, a mutant in this gene was isolated and characterized. In addition, with the aim to find the outer membrane channel for this efflux system, a mutant in PSPTO_4977, a TolC-like gene, was also analyzed. Both mutants were more susceptible to trans-cinnamic and chlorogenic acids and to the flavonoid (+)-catechin, when added to the culture medium. The expression level of both genes increased in the presence of (+)-catechin and, in the case of PSPTO_0820, also in response to trans-cinnamic acid. PSPTO_0820 and PSPTO_4977 mutants were unable to colonize tomato at high population levels. This work evidences the involvement of these two proteins in the resistance to plant antimicrobials, supporting also the importance of chlorogenic acid, trans-cinnamic acid, and (+)-catechin in the tomato plant defense response against P. syringae pv. tomato DC3000 infection. |
url |
https://doi.org/10.1371/journal.pone.0218815 |
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