Effect of Fish Bone Bioactive Peptides on Oxidative, Inflammatory and Pigmentation Processes Triggered by UVB Irradiation in Skin Cells
In the present study, we evaluated for the first time the photoprotective effect of fish bone bioactive peptides (FBBP) preparation isolated from silver carp (<i>Hypophthalmichthys molitrix</i>) discarded tissue using in vitro experimental models of skin cells exposed to ultraviolet B (U...
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doaj-9214a226075c459ea708fbf7f58f0ca72021-05-31T23:11:16ZengMDPI AGMolecules1420-30492021-05-01262691269110.3390/molecules26092691Effect of Fish Bone Bioactive Peptides on Oxidative, Inflammatory and Pigmentation Processes Triggered by UVB Irradiation in Skin CellsAndreea Iosageanu0Daniela Ilie1Oana Craciunescu2Ana-Maria Seciu-Grama3Anca Oancea4Otilia Zarnescu5Ionut Moraru6Florin Oancea7National Institute of R&D for Biological Sciences, 296, Splaiul Independentei, 060031 Bucharest, RomaniaNational Institute of R&D for Biological Sciences, 296, Splaiul Independentei, 060031 Bucharest, RomaniaNational Institute of R&D for Biological Sciences, 296, Splaiul Independentei, 060031 Bucharest, RomaniaNational Institute of R&D for Biological Sciences, 296, Splaiul Independentei, 060031 Bucharest, RomaniaNational Institute of R&D for Biological Sciences, 296, Splaiul Independentei, 060031 Bucharest, RomaniaFaculty of Biology, University of Bucharest, 91-95, Splaiul Independentei, 050095 Bucharest, RomaniaLaboratoarele Medica SRL, 11, Frasinului Street, 075100 Otopeni, RomaniaNational Institute for R&D in Chemistry and Petrochemistry—Icechim, 202, Splaiul Independentei, 060021 Bucharest, RomaniaIn the present study, we evaluated for the first time the photoprotective effect of fish bone bioactive peptides (FBBP) preparation isolated from silver carp (<i>Hypophthalmichthys molitrix</i>) discarded tissue using in vitro experimental models of skin cells exposed to ultraviolet B (UVB) irradiation and stressing agents. FBBP preparation was obtained by papain treatment of minced bones and centrifugal ultrafiltration, and the molecular weight (MW) distribution was characterized by size exclusion and reversed-phase high performance liquid chromatography (RP-HPLC). In vitro assessment of the effect of FBBP pretreatment in UVB-irradiated L929 fibroblasts and HaCaT keratinocytes revealed their cytoprotective activity. Their capacity to efficiently reduce reactive oxygen species (ROS) production and lipid peroxidation varied in a dose-dependent manner, and it was greater in fibroblasts. A decrease of proinflammatory cytokines secretion, in particular of tumor necrosis factor alpha (TNF-α), was found after FBBP pretreatment of THP-1-derived inflamed macrophages. Melanin production and tyrosinase activity investigated in UVB-irradiated Mel-Juso cells were lowered in direct relation to FBBP concentrations. FBBP fractions with high radical scavenging activity were separated by ion exchange chromatography, and two collagenic sequences were identified. All these results offer new scientific data on aquaculture fish bone-derived peptides confirming their ability to control the antioxidant, anti-inflammatory and pigmentation processes developed during UV irradiation of skin cells and recommend their use as valuable natural ingredients of photoprotective cosmeceutical products.https://www.mdpi.com/1420-3049/26/9/2691collagen peptidesfree radicals scavengingantioxidant activitybiological activityproinflammatory cytokinesmelanogenesis |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Andreea Iosageanu Daniela Ilie Oana Craciunescu Ana-Maria Seciu-Grama Anca Oancea Otilia Zarnescu Ionut Moraru Florin Oancea |
spellingShingle |
Andreea Iosageanu Daniela Ilie Oana Craciunescu Ana-Maria Seciu-Grama Anca Oancea Otilia Zarnescu Ionut Moraru Florin Oancea Effect of Fish Bone Bioactive Peptides on Oxidative, Inflammatory and Pigmentation Processes Triggered by UVB Irradiation in Skin Cells Molecules collagen peptides free radicals scavenging antioxidant activity biological activity proinflammatory cytokines melanogenesis |
author_facet |
Andreea Iosageanu Daniela Ilie Oana Craciunescu Ana-Maria Seciu-Grama Anca Oancea Otilia Zarnescu Ionut Moraru Florin Oancea |
author_sort |
Andreea Iosageanu |
title |
Effect of Fish Bone Bioactive Peptides on Oxidative, Inflammatory and Pigmentation Processes Triggered by UVB Irradiation in Skin Cells |
title_short |
Effect of Fish Bone Bioactive Peptides on Oxidative, Inflammatory and Pigmentation Processes Triggered by UVB Irradiation in Skin Cells |
title_full |
Effect of Fish Bone Bioactive Peptides on Oxidative, Inflammatory and Pigmentation Processes Triggered by UVB Irradiation in Skin Cells |
title_fullStr |
Effect of Fish Bone Bioactive Peptides on Oxidative, Inflammatory and Pigmentation Processes Triggered by UVB Irradiation in Skin Cells |
title_full_unstemmed |
Effect of Fish Bone Bioactive Peptides on Oxidative, Inflammatory and Pigmentation Processes Triggered by UVB Irradiation in Skin Cells |
title_sort |
effect of fish bone bioactive peptides on oxidative, inflammatory and pigmentation processes triggered by uvb irradiation in skin cells |
publisher |
MDPI AG |
series |
Molecules |
issn |
1420-3049 |
publishDate |
2021-05-01 |
description |
In the present study, we evaluated for the first time the photoprotective effect of fish bone bioactive peptides (FBBP) preparation isolated from silver carp (<i>Hypophthalmichthys molitrix</i>) discarded tissue using in vitro experimental models of skin cells exposed to ultraviolet B (UVB) irradiation and stressing agents. FBBP preparation was obtained by papain treatment of minced bones and centrifugal ultrafiltration, and the molecular weight (MW) distribution was characterized by size exclusion and reversed-phase high performance liquid chromatography (RP-HPLC). In vitro assessment of the effect of FBBP pretreatment in UVB-irradiated L929 fibroblasts and HaCaT keratinocytes revealed their cytoprotective activity. Their capacity to efficiently reduce reactive oxygen species (ROS) production and lipid peroxidation varied in a dose-dependent manner, and it was greater in fibroblasts. A decrease of proinflammatory cytokines secretion, in particular of tumor necrosis factor alpha (TNF-α), was found after FBBP pretreatment of THP-1-derived inflamed macrophages. Melanin production and tyrosinase activity investigated in UVB-irradiated Mel-Juso cells were lowered in direct relation to FBBP concentrations. FBBP fractions with high radical scavenging activity were separated by ion exchange chromatography, and two collagenic sequences were identified. All these results offer new scientific data on aquaculture fish bone-derived peptides confirming their ability to control the antioxidant, anti-inflammatory and pigmentation processes developed during UV irradiation of skin cells and recommend their use as valuable natural ingredients of photoprotective cosmeceutical products. |
topic |
collagen peptides free radicals scavenging antioxidant activity biological activity proinflammatory cytokines melanogenesis |
url |
https://www.mdpi.com/1420-3049/26/9/2691 |
work_keys_str_mv |
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