Expression of Membrane Progesterone Receptors in Eutopic and Ectopic Endometrium of Women with Endometriosis
Endometriosis is one of the most frequent gynecological diseases in reproductive age women, but its etiology is not completely understood. Endometriosis is characterized by progesterone resistance, which has been explained in part by a decrease in the expression of the intracellular progesterone rec...
Main Authors: | , , , , , , , , , , , , , , , , |
---|---|
Format: | Article |
Language: | English |
Published: |
Hindawi Limited
2020-01-01
|
Series: | BioMed Research International |
Online Access: | http://dx.doi.org/10.1155/2020/2196024 |
id |
doaj-921902a4ebfd493a939856cc9bf56e85 |
---|---|
record_format |
Article |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Edgar Ricardo Vázquez-Martínez Claudia Bello-Alvarez Ana Lorena Hermenegildo-Molina Mario Solís-Paredes Sandra Parra-Hernández Oliver Cruz-Orozco J. Roberto Silvestri-Tomassoni Luis F. Escobar-Ponce Luis A. Hernández-López Christian Reyes-Mayoral Andrea Olguín-Ortega Brenda Sánchez-Ramírez Mauricio Osorio-Caballero Elizabeth García-Gómez Guadalupe Estrada-Gutierrez Marco Cerbón Ignacio Camacho-Arroyo |
spellingShingle |
Edgar Ricardo Vázquez-Martínez Claudia Bello-Alvarez Ana Lorena Hermenegildo-Molina Mario Solís-Paredes Sandra Parra-Hernández Oliver Cruz-Orozco J. Roberto Silvestri-Tomassoni Luis F. Escobar-Ponce Luis A. Hernández-López Christian Reyes-Mayoral Andrea Olguín-Ortega Brenda Sánchez-Ramírez Mauricio Osorio-Caballero Elizabeth García-Gómez Guadalupe Estrada-Gutierrez Marco Cerbón Ignacio Camacho-Arroyo Expression of Membrane Progesterone Receptors in Eutopic and Ectopic Endometrium of Women with Endometriosis BioMed Research International |
author_facet |
Edgar Ricardo Vázquez-Martínez Claudia Bello-Alvarez Ana Lorena Hermenegildo-Molina Mario Solís-Paredes Sandra Parra-Hernández Oliver Cruz-Orozco J. Roberto Silvestri-Tomassoni Luis F. Escobar-Ponce Luis A. Hernández-López Christian Reyes-Mayoral Andrea Olguín-Ortega Brenda Sánchez-Ramírez Mauricio Osorio-Caballero Elizabeth García-Gómez Guadalupe Estrada-Gutierrez Marco Cerbón Ignacio Camacho-Arroyo |
author_sort |
Edgar Ricardo Vázquez-Martínez |
title |
Expression of Membrane Progesterone Receptors in Eutopic and Ectopic Endometrium of Women with Endometriosis |
title_short |
Expression of Membrane Progesterone Receptors in Eutopic and Ectopic Endometrium of Women with Endometriosis |
title_full |
Expression of Membrane Progesterone Receptors in Eutopic and Ectopic Endometrium of Women with Endometriosis |
title_fullStr |
Expression of Membrane Progesterone Receptors in Eutopic and Ectopic Endometrium of Women with Endometriosis |
title_full_unstemmed |
Expression of Membrane Progesterone Receptors in Eutopic and Ectopic Endometrium of Women with Endometriosis |
title_sort |
expression of membrane progesterone receptors in eutopic and ectopic endometrium of women with endometriosis |
publisher |
Hindawi Limited |
series |
BioMed Research International |
issn |
2314-6133 2314-6141 |
publishDate |
2020-01-01 |
description |
Endometriosis is one of the most frequent gynecological diseases in reproductive age women, but its etiology is not completely understood. Endometriosis is characterized by progesterone resistance, which has been explained in part by a decrease in the expression of the intracellular progesterone receptor in the ectopic endometrium. Progesterone action is also mediated by nongenomic mechanisms via membrane progesterone receptors (mPRs) that belong to the class II members of the progesterone and adipoQ receptor (PAQR) family. The aim of the present study was to evaluate the expression at mRNA and protein levels of mPR members in the eutopic and ectopic endometrium of women with endometriosis. Total RNA and total protein were isolated from control endometrium (17 samples), eutopic endometrium (17 samples), and ectopic endometrium (9 samples). The expression of PAQR7 (mPRα), PAQR8 (mPRβ), and PAQR6 (mPRδ) at mRNA and protein levels was evaluated by RT-qPCR and Western blot, whereas PAQR5 (mPRγ) gene expression was evaluated by RT-qPCR. Statistical analysis between comparable groups was performed using one-way ANOVA followed by Tukey’s multiple comparisons test with a confidence interval of 95 %. The analysis of gene expression showed that PAQR7 and PAQR5 expression was lower in both eutopic and ectopic endometrium as compared to the endometrium of women without endometriosis, whereas the expression of PAQR8 and PAQR6 was only reduced in eutopic endometrium. Furthermore, mPRα and mPRβ protein content was decreased in the ectopic endometrium of women with endometriosis. Our results demonstrate a decrease in the expression and protein content of mPRs in eutopic and ectopic endometrium of patients with endometriosis, which could contribute to the progesterone resistance observed in patients with this disease. |
url |
http://dx.doi.org/10.1155/2020/2196024 |
work_keys_str_mv |
AT edgarricardovazquezmartinez expressionofmembraneprogesteronereceptorsineutopicandectopicendometriumofwomenwithendometriosis AT claudiabelloalvarez expressionofmembraneprogesteronereceptorsineutopicandectopicendometriumofwomenwithendometriosis AT analorenahermenegildomolina expressionofmembraneprogesteronereceptorsineutopicandectopicendometriumofwomenwithendometriosis AT mariosolisparedes expressionofmembraneprogesteronereceptorsineutopicandectopicendometriumofwomenwithendometriosis AT sandraparrahernandez expressionofmembraneprogesteronereceptorsineutopicandectopicendometriumofwomenwithendometriosis AT olivercruzorozco expressionofmembraneprogesteronereceptorsineutopicandectopicendometriumofwomenwithendometriosis AT jrobertosilvestritomassoni expressionofmembraneprogesteronereceptorsineutopicandectopicendometriumofwomenwithendometriosis AT luisfescobarponce expressionofmembraneprogesteronereceptorsineutopicandectopicendometriumofwomenwithendometriosis AT luisahernandezlopez expressionofmembraneprogesteronereceptorsineutopicandectopicendometriumofwomenwithendometriosis AT christianreyesmayoral expressionofmembraneprogesteronereceptorsineutopicandectopicendometriumofwomenwithendometriosis AT andreaolguinortega expressionofmembraneprogesteronereceptorsineutopicandectopicendometriumofwomenwithendometriosis AT brendasanchezramirez expressionofmembraneprogesteronereceptorsineutopicandectopicendometriumofwomenwithendometriosis AT mauricioosoriocaballero expressionofmembraneprogesteronereceptorsineutopicandectopicendometriumofwomenwithendometriosis AT elizabethgarciagomez expressionofmembraneprogesteronereceptorsineutopicandectopicendometriumofwomenwithendometriosis AT guadalupeestradagutierrez expressionofmembraneprogesteronereceptorsineutopicandectopicendometriumofwomenwithendometriosis AT marcocerbon expressionofmembraneprogesteronereceptorsineutopicandectopicendometriumofwomenwithendometriosis AT ignaciocamachoarroyo expressionofmembraneprogesteronereceptorsineutopicandectopicendometriumofwomenwithendometriosis |
_version_ |
1715355430244319232 |
spelling |
doaj-921902a4ebfd493a939856cc9bf56e852020-11-25T02:54:18ZengHindawi LimitedBioMed Research International2314-61332314-61412020-01-01202010.1155/2020/21960242196024Expression of Membrane Progesterone Receptors in Eutopic and Ectopic Endometrium of Women with EndometriosisEdgar Ricardo Vázquez-Martínez0Claudia Bello-Alvarez1Ana Lorena Hermenegildo-Molina2Mario Solís-Paredes3Sandra Parra-Hernández4Oliver Cruz-Orozco5J. Roberto Silvestri-Tomassoni6Luis F. Escobar-Ponce7Luis A. Hernández-López8Christian Reyes-Mayoral9Andrea Olguín-Ortega10Brenda Sánchez-Ramírez11Mauricio Osorio-Caballero12Elizabeth García-Gómez13Guadalupe Estrada-Gutierrez14Marco Cerbón15Ignacio Camacho-Arroyo16Unidad de Investigación en Reproducción Humana, Instituto Nacional de Perinatología-Facultad de Química, Universidad Nacional Autónoma de México, Ciudad de México 11000, MexicoUnidad de Investigación en Reproducción Humana, Instituto Nacional de Perinatología-Facultad de Química, Universidad Nacional Autónoma de México, Ciudad de México 11000, MexicoUnidad de Investigación en Reproducción Humana, Instituto Nacional de Perinatología-Facultad de Química, Universidad Nacional Autónoma de México, Ciudad de México 11000, MexicoDepartamento de Genética y Genómica Humana, Instituto Nacional de Perinatología, MexicoDepartamento de Inmunobioquímica, Instituto Nacional de Perinatología, MexicoSubdirección de Reproducción Humana, Instituto Nacional de Perinatología, MexicoDepartamento de Ginecología, Instituto Nacional de Perinatología, MexicoCoordinación de Ginecología Laparoscópica, Instituto Nacional de Perinatología, MexicoDepartamento de Ginecología, Instituto Nacional de Perinatología, MexicoSubdirección de Reproducción Humana, Instituto Nacional de Perinatología, MexicoDepartamento de Ginecología, Instituto Nacional de Perinatología, MexicoDepartamento de Ginecología, Instituto Nacional de Perinatología, MexicoDepartamento de Salud Sexual y Reproducción Humana, Instituto Nacional de Perinatología, MexicoUnidad de Investigación en Reproducción Humana, Consejo Nacional de Ciencia y Tecnología (CONACyT)-Instituto Nacional de Perinatología, Ciudad de México 11000, MexicoDirección de Investigación, Instituto Nacional de Perinatología, Ciudad de México 11000, MexicoUnidad de Investigación en Reproducción Humana, Instituto Nacional de Perinatología-Facultad de Química, Universidad Nacional Autónoma de México, Ciudad de México 11000, MexicoUnidad de Investigación en Reproducción Humana, Instituto Nacional de Perinatología-Facultad de Química, Universidad Nacional Autónoma de México, Ciudad de México 11000, MexicoEndometriosis is one of the most frequent gynecological diseases in reproductive age women, but its etiology is not completely understood. Endometriosis is characterized by progesterone resistance, which has been explained in part by a decrease in the expression of the intracellular progesterone receptor in the ectopic endometrium. Progesterone action is also mediated by nongenomic mechanisms via membrane progesterone receptors (mPRs) that belong to the class II members of the progesterone and adipoQ receptor (PAQR) family. The aim of the present study was to evaluate the expression at mRNA and protein levels of mPR members in the eutopic and ectopic endometrium of women with endometriosis. Total RNA and total protein were isolated from control endometrium (17 samples), eutopic endometrium (17 samples), and ectopic endometrium (9 samples). The expression of PAQR7 (mPRα), PAQR8 (mPRβ), and PAQR6 (mPRδ) at mRNA and protein levels was evaluated by RT-qPCR and Western blot, whereas PAQR5 (mPRγ) gene expression was evaluated by RT-qPCR. Statistical analysis between comparable groups was performed using one-way ANOVA followed by Tukey’s multiple comparisons test with a confidence interval of 95 %. The analysis of gene expression showed that PAQR7 and PAQR5 expression was lower in both eutopic and ectopic endometrium as compared to the endometrium of women without endometriosis, whereas the expression of PAQR8 and PAQR6 was only reduced in eutopic endometrium. Furthermore, mPRα and mPRβ protein content was decreased in the ectopic endometrium of women with endometriosis. Our results demonstrate a decrease in the expression and protein content of mPRs in eutopic and ectopic endometrium of patients with endometriosis, which could contribute to the progesterone resistance observed in patients with this disease.http://dx.doi.org/10.1155/2020/2196024 |