Molecular Profiling of Malignant Pleural Effusions with Next Generation Sequencing (NGS): Evidence that Supports Its Role in Cancer Management
Malignant pleural effusions (MPEs) often develop in advanced cancer patients and confer significant morbidity and mortality. In this review, we evaluated whether molecular profiling of MPEs with next generation sequencing (NGS) could have a role in cancer management, focusing on lung cancer. We revi...
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doaj-921d9746d0b64df5bfcbfff368dc9c942020-11-25T03:52:48ZengMDPI AGJournal of Personalized Medicine2075-44262020-11-011020620610.3390/jpm10040206Molecular Profiling of Malignant Pleural Effusions with Next Generation Sequencing (NGS): Evidence that Supports Its Role in Cancer ManagementGeorgia Ι. Grigoriadou0Stepan M. Esagian1Han Suk Ryu2Ilias P. Nikas31st Department of Medical Oncology, Theageneio Anticancer Hospital, 54007 Thessaloniki, GreeceDepartment of Medicine, School of Health Sciences, Democritus University of Thrace, 68100 Alexandroupolis, GreeceDepartment of Pathology, Seoul National University Hospital, Seoul 03080, KoreaSchool of Medicine, European University of Cyprus, 2404 Nicosia, CyprusMalignant pleural effusions (MPEs) often develop in advanced cancer patients and confer significant morbidity and mortality. In this review, we evaluated whether molecular profiling of MPEs with next generation sequencing (NGS) could have a role in cancer management, focusing on lung cancer. We reviewed and compared the diagnostic performance of pleural fluid liquid biopsy with other types of samples. When applied in MPEs, NGS may have comparable performance with corresponding tissue biopsies, yield higher DNA amount, and detect more genetic aberrations than blood-derived liquid biopsies. NGS in MPEs may also be preferable to plasma liquid biopsy in advanced cancer patients with a MPE and a paucicellular or it could be difficult to obtain tissue/fine-needle aspiration biopsy. Of interest, post-centrifuge supernatant NGS may exhibit superior results compared to cell pellet, cell block or other materials. NGS in MPEs can also guide clinicians in tailoring established therapies and identifying therapy resistance. Evidence is still premature regarding the role of NGS in MPEs from patients with cancers other than lung. We concluded that MPE processing could provide useful prognostic and theranostic information, besides its diagnostic role.https://www.mdpi.com/2075-4426/10/4/206liquid biopsysupernatantpleural fluidnon-small cell lung carcinomaNSCLCprecision oncology |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Georgia Ι. Grigoriadou Stepan M. Esagian Han Suk Ryu Ilias P. Nikas |
spellingShingle |
Georgia Ι. Grigoriadou Stepan M. Esagian Han Suk Ryu Ilias P. Nikas Molecular Profiling of Malignant Pleural Effusions with Next Generation Sequencing (NGS): Evidence that Supports Its Role in Cancer Management Journal of Personalized Medicine liquid biopsy supernatant pleural fluid non-small cell lung carcinoma NSCLC precision oncology |
author_facet |
Georgia Ι. Grigoriadou Stepan M. Esagian Han Suk Ryu Ilias P. Nikas |
author_sort |
Georgia Ι. Grigoriadou |
title |
Molecular Profiling of Malignant Pleural Effusions with Next Generation Sequencing (NGS): Evidence that Supports Its Role in Cancer Management |
title_short |
Molecular Profiling of Malignant Pleural Effusions with Next Generation Sequencing (NGS): Evidence that Supports Its Role in Cancer Management |
title_full |
Molecular Profiling of Malignant Pleural Effusions with Next Generation Sequencing (NGS): Evidence that Supports Its Role in Cancer Management |
title_fullStr |
Molecular Profiling of Malignant Pleural Effusions with Next Generation Sequencing (NGS): Evidence that Supports Its Role in Cancer Management |
title_full_unstemmed |
Molecular Profiling of Malignant Pleural Effusions with Next Generation Sequencing (NGS): Evidence that Supports Its Role in Cancer Management |
title_sort |
molecular profiling of malignant pleural effusions with next generation sequencing (ngs): evidence that supports its role in cancer management |
publisher |
MDPI AG |
series |
Journal of Personalized Medicine |
issn |
2075-4426 |
publishDate |
2020-11-01 |
description |
Malignant pleural effusions (MPEs) often develop in advanced cancer patients and confer significant morbidity and mortality. In this review, we evaluated whether molecular profiling of MPEs with next generation sequencing (NGS) could have a role in cancer management, focusing on lung cancer. We reviewed and compared the diagnostic performance of pleural fluid liquid biopsy with other types of samples. When applied in MPEs, NGS may have comparable performance with corresponding tissue biopsies, yield higher DNA amount, and detect more genetic aberrations than blood-derived liquid biopsies. NGS in MPEs may also be preferable to plasma liquid biopsy in advanced cancer patients with a MPE and a paucicellular or it could be difficult to obtain tissue/fine-needle aspiration biopsy. Of interest, post-centrifuge supernatant NGS may exhibit superior results compared to cell pellet, cell block or other materials. NGS in MPEs can also guide clinicians in tailoring established therapies and identifying therapy resistance. Evidence is still premature regarding the role of NGS in MPEs from patients with cancers other than lung. We concluded that MPE processing could provide useful prognostic and theranostic information, besides its diagnostic role. |
topic |
liquid biopsy supernatant pleural fluid non-small cell lung carcinoma NSCLC precision oncology |
url |
https://www.mdpi.com/2075-4426/10/4/206 |
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