Analysis of the mechanism for enhanced pyrene biodegradation based on the interactions between iron-ions and Rhodococcus ruber strain L9
A slow degradation rate and low transformation efficiency are the main problems in the biodegradation of polycyclic aromatic hydrocarbons (PAHs). This study selected pyrene as the target PAH to investigate the effect of ferrous ion and ferric ion on pyrene degradation. The driving effect and mechani...
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doaj-923b39ff6d0c4d309e661284ab7b91ca2021-10-01T04:44:46ZengElsevierEcotoxicology and Environmental Safety0147-65132021-12-01225112789Analysis of the mechanism for enhanced pyrene biodegradation based on the interactions between iron-ions and Rhodococcus ruber strain L9Jing Liu0Ai-Ning Zhang1Yong-Jun Liu2Zhe Liu3Yu Liu4Xi-Jun Wu5School of Civil Engineering, Yulin University, Yulin 719000, China; Key Lab of Northwest Water Resource, Ecology and Environment, Ministry of Education, Xi’an University of Architecture and Technology, No. 13 Yanta Road, Xi’an 710055, ChinaKey Lab of Northwest Water Resource, Ecology and Environment, Ministry of Education, Xi’an University of Architecture and Technology, No. 13 Yanta Road, Xi’an 710055, ChinaKey Lab of Northwest Water Resource, Ecology and Environment, Ministry of Education, Xi’an University of Architecture and Technology, No. 13 Yanta Road, Xi’an 710055, China; Corresponding author.Key Lab of Northwest Water Resource, Ecology and Environment, Ministry of Education, Xi’an University of Architecture and Technology, No. 13 Yanta Road, Xi’an 710055, ChinaSchool of Petroleum and Environment Engineering, Yanan University, Yanan 716000, ChinaSchool of Civil Engineering, Yulin University, Yulin 719000, ChinaA slow degradation rate and low transformation efficiency are the main problems in the biodegradation of polycyclic aromatic hydrocarbons (PAHs). This study selected pyrene as the target PAH to investigate the effect of ferrous ion and ferric ion on pyrene degradation. The driving effect and mechanism, including the interaction between pyrene and iron ions and the bacterial physiological response during the biodegradation process by Rhodococcus ruber strain L9, were investigated. The results showed that iron ions did not enhance bacterial growth but improved bacteria’s pyrene removal capacity, contributing to the total efficiency of pyrene biodegradation. The process started with an initial formation of “cation-π” between Fe (III) and pyrene, which subsequently drove the pyrene removal process and accelerated the bacterial metabolic process. Moreover, a significant increase in the protein concentration, catechol dioxygenase (C12O and C23O) activities, and intracellular protein regulation in crude enzyme solution indicate a positive response of the bacteria during the iron ion-enhanced pyrene degradation process.http://www.sciencedirect.com/science/article/pii/S0147651321009015Iron ion - drivenPyreneBiodegradationRhodococcus ruber strain L9Mechanism |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Jing Liu Ai-Ning Zhang Yong-Jun Liu Zhe Liu Yu Liu Xi-Jun Wu |
spellingShingle |
Jing Liu Ai-Ning Zhang Yong-Jun Liu Zhe Liu Yu Liu Xi-Jun Wu Analysis of the mechanism for enhanced pyrene biodegradation based on the interactions between iron-ions and Rhodococcus ruber strain L9 Ecotoxicology and Environmental Safety Iron ion - driven Pyrene Biodegradation Rhodococcus ruber strain L9 Mechanism |
author_facet |
Jing Liu Ai-Ning Zhang Yong-Jun Liu Zhe Liu Yu Liu Xi-Jun Wu |
author_sort |
Jing Liu |
title |
Analysis of the mechanism for enhanced pyrene biodegradation based on the interactions between iron-ions and Rhodococcus ruber strain L9 |
title_short |
Analysis of the mechanism for enhanced pyrene biodegradation based on the interactions between iron-ions and Rhodococcus ruber strain L9 |
title_full |
Analysis of the mechanism for enhanced pyrene biodegradation based on the interactions between iron-ions and Rhodococcus ruber strain L9 |
title_fullStr |
Analysis of the mechanism for enhanced pyrene biodegradation based on the interactions between iron-ions and Rhodococcus ruber strain L9 |
title_full_unstemmed |
Analysis of the mechanism for enhanced pyrene biodegradation based on the interactions between iron-ions and Rhodococcus ruber strain L9 |
title_sort |
analysis of the mechanism for enhanced pyrene biodegradation based on the interactions between iron-ions and rhodococcus ruber strain l9 |
publisher |
Elsevier |
series |
Ecotoxicology and Environmental Safety |
issn |
0147-6513 |
publishDate |
2021-12-01 |
description |
A slow degradation rate and low transformation efficiency are the main problems in the biodegradation of polycyclic aromatic hydrocarbons (PAHs). This study selected pyrene as the target PAH to investigate the effect of ferrous ion and ferric ion on pyrene degradation. The driving effect and mechanism, including the interaction between pyrene and iron ions and the bacterial physiological response during the biodegradation process by Rhodococcus ruber strain L9, were investigated. The results showed that iron ions did not enhance bacterial growth but improved bacteria’s pyrene removal capacity, contributing to the total efficiency of pyrene biodegradation. The process started with an initial formation of “cation-π” between Fe (III) and pyrene, which subsequently drove the pyrene removal process and accelerated the bacterial metabolic process. Moreover, a significant increase in the protein concentration, catechol dioxygenase (C12O and C23O) activities, and intracellular protein regulation in crude enzyme solution indicate a positive response of the bacteria during the iron ion-enhanced pyrene degradation process. |
topic |
Iron ion - driven Pyrene Biodegradation Rhodococcus ruber strain L9 Mechanism |
url |
http://www.sciencedirect.com/science/article/pii/S0147651321009015 |
work_keys_str_mv |
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