Recognition of Thiols in Living Cells and Zebrafish Using an Imidazo[1,5-α]pyridine-Derivative Indicator

Recognition of Thiols in Living Cells and Zebrafish Using an Imidazo[1,5-α]pyridine-Derivative Indicator

A new cyan fluorescent probe, <b>MIPY-DNBS</b>, using an imidazo[1,5-&#945;]pyridine derivative as the fluorophore and 2,4-dinitrobenzensufonate as the recognition site for the selective detection of thiols (Cys, GSH, and Hcy), was designed and synthesized. Probe <b>MIPY-DNBS&l...

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Bibliographic Details
Main Authors: Song Chen, Peng Hou, Jingwen Sun, Haijun Wang, Lei Liu
Format: Article
Language:English
Published: MDPI AG 2019-09-01
Series:Molecules
Subjects:
large Stokes shift
zebrafish
thiols
cell imaging
imidazo[1,5-α]pyridine
Online Access:https://www.mdpi.com/1420-3049/24/18/3328
Description
Summary:A new cyan fluorescent probe, <b>MIPY-DNBS</b>, using an imidazo[1,5-&#945;]pyridine derivative as the fluorophore and 2,4-dinitrobenzensufonate as the recognition site for the selective detection of thiols (Cys, GSH, and Hcy), was designed and synthesized. Probe <b>MIPY-DNBS</b> exhibited a 172 nm Stokes shift, a fast response time (400 s), low cytotoxicity, low detection limits (12.7 nM for Cys), and excellent selectively in the detection of thiols. In addition, <b>MIPY-DNBS</b> was successfully applied to imaging thiols in living MCF-7 cells and zebrafish.
ISSN:1420-3049

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