Recognition of Thiols in Living Cells and Zebrafish Using an Imidazo[1,5-α]pyridine-Derivative Indicator

A new cyan fluorescent probe, <b>MIPY-DNBS</b>, using an imidazo[1,5-α]pyridine derivative as the fluorophore and 2,4-dinitrobenzensufonate as the recognition site for the selective detection of thiols (Cys, GSH, and Hcy), was designed and synthesized. Probe <b>MIPY-DNBS&l...

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Main Authors:	Song Chen, Peng Hou, Jingwen Sun, Haijun Wang, Lei Liu
Format:	Article
Language:	English
Published:	MDPI AG 2019-09-01
Series:	Molecules
Subjects:	large Stokes shift zebrafish thiols cell imaging imidazo[1,5-α]pyridine
Online Access:	https://www.mdpi.com/1420-3049/24/18/3328

id	doaj-92712510c2024f728cc9a721858e3190
record_format	Article
spelling	doaj-92712510c2024f728cc9a721858e31902020-11-25T01:08:42ZengMDPI AGMolecules1420-30492019-09-012418332810.3390/molecules24183328molecules24183328Recognition of Thiols in Living Cells and Zebrafish Using an Imidazo[1,5-α]pyridine-Derivative IndicatorSong Chen0Peng Hou1Jingwen Sun2Haijun Wang3Lei Liu4College of Pharmacy, Qiqihar Medical University, Qiqihar 161006, ChinaCollege of Pharmacy, Qiqihar Medical University, Qiqihar 161006, ChinaCollege of Pharmacy, Qiqihar Medical University, Qiqihar 161006, ChinaCollege of Pharmacy, Qiqihar Medical University, Qiqihar 161006, ChinaCollege of Pharmacy, Qiqihar Medical University, Qiqihar 161006, ChinaA new cyan fluorescent probe, <b>MIPY-DNBS</b>, using an imidazo[1,5-α]pyridine derivative as the fluorophore and 2,4-dinitrobenzensufonate as the recognition site for the selective detection of thiols (Cys, GSH, and Hcy), was designed and synthesized. Probe <b>MIPY-DNBS</b> exhibited a 172 nm Stokes shift, a fast response time (400 s), low cytotoxicity, low detection limits (12.7 nM for Cys), and excellent selectively in the detection of thiols. In addition, <b>MIPY-DNBS</b> was successfully applied to imaging thiols in living MCF-7 cells and zebrafish.https://www.mdpi.com/1420-3049/24/18/3328large Stokes shiftzebrafishthiolscell imagingimidazo[1,5-α]pyridine
collection	DOAJ
language	English
format	Article
sources	DOAJ
author	Song Chen Peng Hou Jingwen Sun Haijun Wang Lei Liu
spellingShingle	Song Chen Peng Hou Jingwen Sun Haijun Wang Lei Liu Recognition of Thiols in Living Cells and Zebrafish Using an Imidazo[1,5-α]pyridine-Derivative Indicator Molecules large Stokes shift zebrafish thiols cell imaging imidazo[1,5-α]pyridine
author_facet	Song Chen Peng Hou Jingwen Sun Haijun Wang Lei Liu
author_sort	Song Chen
title	Recognition of Thiols in Living Cells and Zebrafish Using an Imidazo[1,5-α]pyridine-Derivative Indicator
title_short	Recognition of Thiols in Living Cells and Zebrafish Using an Imidazo[1,5-α]pyridine-Derivative Indicator
title_full	Recognition of Thiols in Living Cells and Zebrafish Using an Imidazo[1,5-α]pyridine-Derivative Indicator
title_fullStr	Recognition of Thiols in Living Cells and Zebrafish Using an Imidazo[1,5-α]pyridine-Derivative Indicator
title_full_unstemmed	Recognition of Thiols in Living Cells and Zebrafish Using an Imidazo[1,5-α]pyridine-Derivative Indicator
title_sort	recognition of thiols in living cells and zebrafish using an imidazo[1,5-α]pyridine-derivative indicator
publisher	MDPI AG
series	Molecules
issn	1420-3049
publishDate	2019-09-01
description	A new cyan fluorescent probe, <b>MIPY-DNBS</b>, using an imidazo[1,5-α]pyridine derivative as the fluorophore and 2,4-dinitrobenzensufonate as the recognition site for the selective detection of thiols (Cys, GSH, and Hcy), was designed and synthesized. Probe <b>MIPY-DNBS</b> exhibited a 172 nm Stokes shift, a fast response time (400 s), low cytotoxicity, low detection limits (12.7 nM for Cys), and excellent selectively in the detection of thiols. In addition, <b>MIPY-DNBS</b> was successfully applied to imaging thiols in living MCF-7 cells and zebrafish.
topic	large Stokes shift zebrafish thiols cell imaging imidazo[1,5-α]pyridine
url	https://www.mdpi.com/1420-3049/24/18/3328
work_keys_str_mv	AT songchen recognitionofthiolsinlivingcellsandzebrafishusinganimidazo15apyridinederivativeindicator AT penghou recognitionofthiolsinlivingcellsandzebrafishusinganimidazo15apyridinederivativeindicator AT jingwensun recognitionofthiolsinlivingcellsandzebrafishusinganimidazo15apyridinederivativeindicator AT haijunwang recognitionofthiolsinlivingcellsandzebrafishusinganimidazo15apyridinederivativeindicator AT leiliu recognitionofthiolsinlivingcellsandzebrafishusinganimidazo15apyridinederivativeindicator
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Recognition of Thiols in Living Cells and Zebrafish Using an Imidazo[1,5-α]pyridine-Derivative Indicator

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