P40 and P75 Are Singular Functional Muramidases Present in the Lactobacillus casei /paracasei/rhamnosus Taxon
Lactobacillus casei and Lactobacillus rhamnosus proteins P40 and P75 belong to a large family of secreted cell wall proteins that contain a carboxy(C)-terminal CHAP or NlpC/P60 superfamily domains. In addition to their peptidoglycan hydrolases activity, proteins in this family are specific antigens...
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doaj-927a7ae5fa594bedbe7e54da3f370dcc2020-11-24T21:28:32ZengFrontiers Media S.A.Frontiers in Microbiology1664-302X2019-06-011010.3389/fmicb.2019.01420454589P40 and P75 Are Singular Functional Muramidases Present in the Lactobacillus casei /paracasei/rhamnosus TaxonChristine Bäuerl0Gulyaim Abitayeva1Gulyaim Abitayeva2Sebastián Sosa-Carrillo3Ana Mencher-Beltrán4Noemí Navarro-Lleó5José M. Coll-Marqués6Manuel Zúñiga-Cabrera7Serik Shaikhin8Serik Shaikhin9Gaspar Pérez-Martinez10Departamento de Biotecnología, Instituto de Agroquímica y Tecnología de Alimentos (C.S.I.C.), Valencia, SpainDepartment of Microbiology, L.N. Gumilyov Eurasian National University, Astana, KazakhstanLaboratory of Genetics and Biochemistry of Microorganisms, Republican Collection of Microorganisms at Science Committee of Ministry of Education and Science RK, Astana, KazakhstanComputational Biology Department, Inria, Institut Pasteur and Université Paris Diderot, Paris, FranceInstituto de Ciencias de la Vid y del Vino (CSIC), Logroño, SpainDepartamento de Biotecnología, Instituto de Agroquímica y Tecnología de Alimentos (C.S.I.C.), Valencia, SpainDepartamento de Biotecnología, Instituto de Agroquímica y Tecnología de Alimentos (C.S.I.C.), Valencia, SpainDepartamento de Biotecnología, Instituto de Agroquímica y Tecnología de Alimentos (C.S.I.C.), Valencia, SpainDepartment of Microbiology, L.N. Gumilyov Eurasian National University, Astana, KazakhstanLaboratory of Genetics and Biochemistry of Microorganisms, Republican Collection of Microorganisms at Science Committee of Ministry of Education and Science RK, Astana, KazakhstanDepartamento de Biotecnología, Instituto de Agroquímica y Tecnología de Alimentos (C.S.I.C.), Valencia, SpainLactobacillus casei and Lactobacillus rhamnosus proteins P40 and P75 belong to a large family of secreted cell wall proteins that contain a carboxy(C)-terminal CHAP or NlpC/P60 superfamily domains. In addition to their peptidoglycan hydrolases activity, proteins in this family are specific antigens of pathogens, frequently responsible of interactions with the host. L. rhamnosus GG and L. casei BL23 purified P40 and P75 proteins have antiapoptotic activity by inducing the EGF/Akt pathway. The aim of this work was to study the genetics, phylogeny and dissemination of this family of proteins in the genus Lactobacillus as well as their characteristics and likely function. The scrutiny of their DNA encoding sequences revealed the presence of minisatellite DNA in the P75 encoding gene of L. casei/paracasei strains (cmuB) with intraspecific indels that gave raise to four different alleles (cmuB1–4), which are exclusive of this species. Phylogenic analyses suggest that both proteins are present mainly in the L. casei and Lactobacillus sakei phylogenomic groups. A P40 ancestral gene was possibly present in the common ancestor of Enterococcaceae, Lactobacillaceae and Streptococcaceae. P75 is also present in L. casei and L. sakei groups, but its evolution is difficult to explain only by vertical transmission. Antibodies raised against the N-terminal regions of P40 and P75 improved their immunological detection in culture supernatants as they recognized almost exclusively proteins of L. casei/paracasei/rhamnosus strains, highlighting their structural similarity, that allowed to detect them in different fermented dairy products that contained probiotic L. casei strains. Purified P40 and P75 proteins showed no evident lytic activity but they complemented L. casei BL23 cmuA and cmuB defective mutants, respectively, thus proving that they actively participate in cell division.https://www.frontiersin.org/article/10.3389/fmicb.2019.01420/fullP40P75secreted muramidasesgenetic analysisminisatellitephylogeny |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Christine Bäuerl Gulyaim Abitayeva Gulyaim Abitayeva Sebastián Sosa-Carrillo Ana Mencher-Beltrán Noemí Navarro-Lleó José M. Coll-Marqués Manuel Zúñiga-Cabrera Serik Shaikhin Serik Shaikhin Gaspar Pérez-Martinez |
spellingShingle |
Christine Bäuerl Gulyaim Abitayeva Gulyaim Abitayeva Sebastián Sosa-Carrillo Ana Mencher-Beltrán Noemí Navarro-Lleó José M. Coll-Marqués Manuel Zúñiga-Cabrera Serik Shaikhin Serik Shaikhin Gaspar Pérez-Martinez P40 and P75 Are Singular Functional Muramidases Present in the Lactobacillus casei /paracasei/rhamnosus Taxon Frontiers in Microbiology P40 P75 secreted muramidases genetic analysis minisatellite phylogeny |
author_facet |
Christine Bäuerl Gulyaim Abitayeva Gulyaim Abitayeva Sebastián Sosa-Carrillo Ana Mencher-Beltrán Noemí Navarro-Lleó José M. Coll-Marqués Manuel Zúñiga-Cabrera Serik Shaikhin Serik Shaikhin Gaspar Pérez-Martinez |
author_sort |
Christine Bäuerl |
title |
P40 and P75 Are Singular Functional Muramidases Present in the Lactobacillus casei /paracasei/rhamnosus Taxon |
title_short |
P40 and P75 Are Singular Functional Muramidases Present in the Lactobacillus casei /paracasei/rhamnosus Taxon |
title_full |
P40 and P75 Are Singular Functional Muramidases Present in the Lactobacillus casei /paracasei/rhamnosus Taxon |
title_fullStr |
P40 and P75 Are Singular Functional Muramidases Present in the Lactobacillus casei /paracasei/rhamnosus Taxon |
title_full_unstemmed |
P40 and P75 Are Singular Functional Muramidases Present in the Lactobacillus casei /paracasei/rhamnosus Taxon |
title_sort |
p40 and p75 are singular functional muramidases present in the lactobacillus casei /paracasei/rhamnosus taxon |
publisher |
Frontiers Media S.A. |
series |
Frontiers in Microbiology |
issn |
1664-302X |
publishDate |
2019-06-01 |
description |
Lactobacillus casei and Lactobacillus rhamnosus proteins P40 and P75 belong to a large family of secreted cell wall proteins that contain a carboxy(C)-terminal CHAP or NlpC/P60 superfamily domains. In addition to their peptidoglycan hydrolases activity, proteins in this family are specific antigens of pathogens, frequently responsible of interactions with the host. L. rhamnosus GG and L. casei BL23 purified P40 and P75 proteins have antiapoptotic activity by inducing the EGF/Akt pathway. The aim of this work was to study the genetics, phylogeny and dissemination of this family of proteins in the genus Lactobacillus as well as their characteristics and likely function. The scrutiny of their DNA encoding sequences revealed the presence of minisatellite DNA in the P75 encoding gene of L. casei/paracasei strains (cmuB) with intraspecific indels that gave raise to four different alleles (cmuB1–4), which are exclusive of this species. Phylogenic analyses suggest that both proteins are present mainly in the L. casei and Lactobacillus sakei phylogenomic groups. A P40 ancestral gene was possibly present in the common ancestor of Enterococcaceae, Lactobacillaceae and Streptococcaceae. P75 is also present in L. casei and L. sakei groups, but its evolution is difficult to explain only by vertical transmission. Antibodies raised against the N-terminal regions of P40 and P75 improved their immunological detection in culture supernatants as they recognized almost exclusively proteins of L. casei/paracasei/rhamnosus strains, highlighting their structural similarity, that allowed to detect them in different fermented dairy products that contained probiotic L. casei strains. Purified P40 and P75 proteins showed no evident lytic activity but they complemented L. casei BL23 cmuA and cmuB defective mutants, respectively, thus proving that they actively participate in cell division. |
topic |
P40 P75 secreted muramidases genetic analysis minisatellite phylogeny |
url |
https://www.frontiersin.org/article/10.3389/fmicb.2019.01420/full |
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