Altered protein expression in serum from endometrial hyperplasia and carcinoma patients
<p>Abstract</p> <p>Background</p> <p>Endometrial carcinoma is one of the most common gynecological malignancies in women. The diagnosis of the disease at early or premalignant stages is crucial for the patient's prognosis. To date, diagnosis and follow-up of endome...
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doaj-9299e3fc07e044e781bacb633bd38f332020-11-24T20:51:44ZengBMCJournal of Hematology & Oncology1756-87222011-04-01411510.1186/1756-8722-4-15Altered protein expression in serum from endometrial hyperplasia and carcinoma patientsCong QingZhang Xiao-yanHuang Yi-pingJin HongCao RuiWang Yi-shengHe Yi-fengXu Cong-jian<p>Abstract</p> <p>Background</p> <p>Endometrial carcinoma is one of the most common gynecological malignancies in women. The diagnosis of the disease at early or premalignant stages is crucial for the patient's prognosis. To date, diagnosis and follow-up of endometrial carcinoma and hyperplasia require invasive procedures. Therefore, there is considerable demand for the identification of biomarkers to allow non-invasive detection of these conditions.</p> <p>Methods</p> <p>In this study, we performed a quantitative proteomics analysis on serum samples from simple endometrial hyperplasia, complex endometrial hyperplasia, atypical endometrial hyperplasia, and endometrial carcinoma patients, as well as healthy women. Serum samples were first depleted of high-abundance proteins, labeled with isobaric tags (iTRAQ™), and then analyzed via two-dimensional liquid chromatography and tandem mass spectrometry. Protein identification and quantitation information were acquired by comparing the mass spectrometry data against the International Protein Index Database using ProteinPilot software. Bioinformatics annotation of identified proteins was performed by searching against the PANTHER database.</p> <p>Results</p> <p>In total, 74 proteins were identified and quantified in serum samples from endometrial lesion patients and healthy women. Using a 1.6-fold change as the benchmark, 12 proteins showed significantly altered expression levels in at least one disease group compared with healthy women. Among them, 7 proteins were found, for the first time, to be differentially expressed in atypical endometrial hyperplasia. These proteins are orosomucoid 1, haptoglobin, SERPINC 1, alpha-1-antichymotrypsin, apolipoprotein A-IV, inter-alpha-trypsin inhibitor heavy chain H4, and histidine-rich glycoprotein.</p> <p>Conclusions</p> <p>The differentially expressed proteins we discovered in this study may serve as biomarkers in the diagnosis and follow-up of endometrial hyperplasia and endometrial carcinoma.</p> http://www.jhoonline.org/content/4/1/15 |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Cong Qing Zhang Xiao-yan Huang Yi-ping Jin Hong Cao Rui Wang Yi-sheng He Yi-feng Xu Cong-jian |
spellingShingle |
Cong Qing Zhang Xiao-yan Huang Yi-ping Jin Hong Cao Rui Wang Yi-sheng He Yi-feng Xu Cong-jian Altered protein expression in serum from endometrial hyperplasia and carcinoma patients Journal of Hematology & Oncology |
author_facet |
Cong Qing Zhang Xiao-yan Huang Yi-ping Jin Hong Cao Rui Wang Yi-sheng He Yi-feng Xu Cong-jian |
author_sort |
Cong Qing |
title |
Altered protein expression in serum from endometrial hyperplasia and carcinoma patients |
title_short |
Altered protein expression in serum from endometrial hyperplasia and carcinoma patients |
title_full |
Altered protein expression in serum from endometrial hyperplasia and carcinoma patients |
title_fullStr |
Altered protein expression in serum from endometrial hyperplasia and carcinoma patients |
title_full_unstemmed |
Altered protein expression in serum from endometrial hyperplasia and carcinoma patients |
title_sort |
altered protein expression in serum from endometrial hyperplasia and carcinoma patients |
publisher |
BMC |
series |
Journal of Hematology & Oncology |
issn |
1756-8722 |
publishDate |
2011-04-01 |
description |
<p>Abstract</p> <p>Background</p> <p>Endometrial carcinoma is one of the most common gynecological malignancies in women. The diagnosis of the disease at early or premalignant stages is crucial for the patient's prognosis. To date, diagnosis and follow-up of endometrial carcinoma and hyperplasia require invasive procedures. Therefore, there is considerable demand for the identification of biomarkers to allow non-invasive detection of these conditions.</p> <p>Methods</p> <p>In this study, we performed a quantitative proteomics analysis on serum samples from simple endometrial hyperplasia, complex endometrial hyperplasia, atypical endometrial hyperplasia, and endometrial carcinoma patients, as well as healthy women. Serum samples were first depleted of high-abundance proteins, labeled with isobaric tags (iTRAQ™), and then analyzed via two-dimensional liquid chromatography and tandem mass spectrometry. Protein identification and quantitation information were acquired by comparing the mass spectrometry data against the International Protein Index Database using ProteinPilot software. Bioinformatics annotation of identified proteins was performed by searching against the PANTHER database.</p> <p>Results</p> <p>In total, 74 proteins were identified and quantified in serum samples from endometrial lesion patients and healthy women. Using a 1.6-fold change as the benchmark, 12 proteins showed significantly altered expression levels in at least one disease group compared with healthy women. Among them, 7 proteins were found, for the first time, to be differentially expressed in atypical endometrial hyperplasia. These proteins are orosomucoid 1, haptoglobin, SERPINC 1, alpha-1-antichymotrypsin, apolipoprotein A-IV, inter-alpha-trypsin inhibitor heavy chain H4, and histidine-rich glycoprotein.</p> <p>Conclusions</p> <p>The differentially expressed proteins we discovered in this study may serve as biomarkers in the diagnosis and follow-up of endometrial hyperplasia and endometrial carcinoma.</p> |
url |
http://www.jhoonline.org/content/4/1/15 |
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