Ablation of KDM2A Inhibits Preadipocyte Proliferation and Promotes Adipogenic Differentiation
Epigenetic signals and chromatin-modifying proteins play critical roles in adipogenesis, which determines the risk of obesity and which has recently attracted increasing interest. Histone demethylase 2A (KDM2A) is an important component of histone demethylase; however, its direct effect on fat depos...
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doaj-92a3550e964a43d8bbd180a83447e2ab2021-09-26T00:22:41ZengMDPI AGInternational Journal of Molecular Sciences1661-65961422-00672021-09-01229759975910.3390/ijms22189759Ablation of KDM2A Inhibits Preadipocyte Proliferation and Promotes Adipogenic DifferentiationYonglin Hua0Yongqi Yue1Dan Zhao2Yan Ma3Yan Xiong4Xianrong Xiong5Jian Li6Key Laboratory of Qinghai-Tibetan Plateau Animal Genetic Resource Reservation and Utilization, Ministry of Education, Southwest Minzu University, Chengdu 610041, ChinaKey Laboratory of Qinghai-Tibetan Plateau Animal Genetic Resource Reservation and Utilization, Ministry of Education, Southwest Minzu University, Chengdu 610041, ChinaKey Laboratory of Qinghai-Tibetan Plateau Animal Genetic Resource Reservation and Utilization, Ministry of Education, Southwest Minzu University, Chengdu 610041, ChinaKey Laboratory of Qinghai-Tibetan Plateau Animal Genetic Resource Reservation and Utilization, Ministry of Education, Southwest Minzu University, Chengdu 610041, ChinaKey Laboratory of Qinghai-Tibetan Plateau Animal Genetic Resource Reservation and Utilization, Ministry of Education, Southwest Minzu University, Chengdu 610041, ChinaKey Laboratory of Qinghai-Tibetan Plateau Animal Genetic Resource Reservation and Utilization, Ministry of Education, Southwest Minzu University, Chengdu 610041, ChinaKey Laboratory of Qinghai-Tibetan Plateau Animal Genetic Resource Reservation and Utilization, Ministry of Education, Southwest Minzu University, Chengdu 610041, ChinaEpigenetic signals and chromatin-modifying proteins play critical roles in adipogenesis, which determines the risk of obesity and which has recently attracted increasing interest. Histone demethylase 2A (KDM2A) is an important component of histone demethylase; however, its direct effect on fat deposition remains unclear. Here, a KDM2A loss of function was performed using two unbiased methods, small interfering RNA (siRNA) and Cre-Loxp recombinase systems, to reveal its function in adipogenesis. The results show that the knockdown of KDM2A by siRNAs inhibited the proliferation capacity of 3T3-L1 preadipocytes. Furthermore, the promotion of preadipocyte differentiation was observed in siRNA-treated cells, manifested by the increasing content of lipid droplets and the expression level of adipogenic-related genes. Consistently, the genetic deletion of KDM2A by Adipoq-Cre in primary adipocytes exhibited similar phenotypes to those of 3T3-L1 preadipocytes. Interestingly, the knockdown of KDM2A upregulates the expression level of Transportin 1(TNPO1), which in turn may induce the nuclear translocation of PPARγ and the accumulation of lipid droplets. In conclusion, the ablation of KDM2A inhibits preadipocyte proliferation and promotes its adipogenic differentiation. This work provides direct evidence of the exact role of KDM2A in fat deposition and provides theoretical support for obesity therapy that targets KDM2A.https://www.mdpi.com/1422-0067/22/18/9759KDM2Afat depositionpreadipocytesproliferationdifferentiationTNPO1 |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Yonglin Hua Yongqi Yue Dan Zhao Yan Ma Yan Xiong Xianrong Xiong Jian Li |
spellingShingle |
Yonglin Hua Yongqi Yue Dan Zhao Yan Ma Yan Xiong Xianrong Xiong Jian Li Ablation of KDM2A Inhibits Preadipocyte Proliferation and Promotes Adipogenic Differentiation International Journal of Molecular Sciences KDM2A fat deposition preadipocytes proliferation differentiation TNPO1 |
author_facet |
Yonglin Hua Yongqi Yue Dan Zhao Yan Ma Yan Xiong Xianrong Xiong Jian Li |
author_sort |
Yonglin Hua |
title |
Ablation of KDM2A Inhibits Preadipocyte Proliferation and Promotes Adipogenic Differentiation |
title_short |
Ablation of KDM2A Inhibits Preadipocyte Proliferation and Promotes Adipogenic Differentiation |
title_full |
Ablation of KDM2A Inhibits Preadipocyte Proliferation and Promotes Adipogenic Differentiation |
title_fullStr |
Ablation of KDM2A Inhibits Preadipocyte Proliferation and Promotes Adipogenic Differentiation |
title_full_unstemmed |
Ablation of KDM2A Inhibits Preadipocyte Proliferation and Promotes Adipogenic Differentiation |
title_sort |
ablation of kdm2a inhibits preadipocyte proliferation and promotes adipogenic differentiation |
publisher |
MDPI AG |
series |
International Journal of Molecular Sciences |
issn |
1661-6596 1422-0067 |
publishDate |
2021-09-01 |
description |
Epigenetic signals and chromatin-modifying proteins play critical roles in adipogenesis, which determines the risk of obesity and which has recently attracted increasing interest. Histone demethylase 2A (KDM2A) is an important component of histone demethylase; however, its direct effect on fat deposition remains unclear. Here, a KDM2A loss of function was performed using two unbiased methods, small interfering RNA (siRNA) and Cre-Loxp recombinase systems, to reveal its function in adipogenesis. The results show that the knockdown of KDM2A by siRNAs inhibited the proliferation capacity of 3T3-L1 preadipocytes. Furthermore, the promotion of preadipocyte differentiation was observed in siRNA-treated cells, manifested by the increasing content of lipid droplets and the expression level of adipogenic-related genes. Consistently, the genetic deletion of KDM2A by Adipoq-Cre in primary adipocytes exhibited similar phenotypes to those of 3T3-L1 preadipocytes. Interestingly, the knockdown of KDM2A upregulates the expression level of Transportin 1(TNPO1), which in turn may induce the nuclear translocation of PPARγ and the accumulation of lipid droplets. In conclusion, the ablation of KDM2A inhibits preadipocyte proliferation and promotes its adipogenic differentiation. This work provides direct evidence of the exact role of KDM2A in fat deposition and provides theoretical support for obesity therapy that targets KDM2A. |
topic |
KDM2A fat deposition preadipocytes proliferation differentiation TNPO1 |
url |
https://www.mdpi.com/1422-0067/22/18/9759 |
work_keys_str_mv |
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