Angiotensin II: immunohistochemical study in Sardinian pterygium
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PAGEPress Publications
2014-07-01
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Series: | European Journal of Histochemistry |
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Online Access: | http://www.ejh.it/index.php/ejh/article/view/2426 |
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Article |
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DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
P. Demurtas M. Corrias I. Zucca C. Maxia F. Piras P. Sirigu M.T. Perra |
spellingShingle |
P. Demurtas M. Corrias I. Zucca C. Maxia F. Piras P. Sirigu M.T. Perra Angiotensin II: immunohistochemical study in Sardinian pterygium European Journal of Histochemistry Renin-angiotensin system (RAS), angiotensin II (Ang II), intracellular, immunohistochemistry. |
author_facet |
P. Demurtas M. Corrias I. Zucca C. Maxia F. Piras P. Sirigu M.T. Perra |
author_sort |
P. Demurtas |
title |
Angiotensin II: immunohistochemical study in Sardinian pterygium |
title_short |
Angiotensin II: immunohistochemical study in Sardinian pterygium |
title_full |
Angiotensin II: immunohistochemical study in Sardinian pterygium |
title_fullStr |
Angiotensin II: immunohistochemical study in Sardinian pterygium |
title_full_unstemmed |
Angiotensin II: immunohistochemical study in Sardinian pterygium |
title_sort |
angiotensin ii: immunohistochemical study in sardinian pterygium |
publisher |
PAGEPress Publications |
series |
European Journal of Histochemistry |
issn |
1121-760X 2038-8306 |
publishDate |
2014-07-01 |
description |
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</xml><![endif]--><p>The angiotensin II (Ang II) is the principal effector peptide of the RAS system. It has a pleiotropic effect and, beside its <em>physiological</em> role, it has the property to stimulate angiogenesis and activate multiple signalling pathways related to cell proliferation. The purpose of the study was to determinate the Ang II expression and localization in Sardinian pterygium and normal conjunctiva by immunohistochemistry, and its possible involvement in the development and progression of the disease. Twenty-three pterygiums and eleven normal conjunctiva specimens obtained from Sardinian patients, were processed for paraffin embedding and assessed for the immunohistochemical revelation of Ang II. Significant Ang II expression was identified in pterygium and conjuntica. Particularly, thirteen pterygium specimens (n=13) displayed exclusively moderate to strong nuclear staining; some specimens (n=5) showed exclusively a moderate cytoplasmatic immunoreactivity, and few specimens (n=2) displayed moderate to strong immunoreactivity in both cytoplasm and nucleus. Statistical significance difference in respect of nuclear and cytoplasmatic localization was observed between normal conjunctiva and pterygium (P=0.038).The results showed a predominant intranuclear localization of Ang II in pterygium epithelial cells, in spite of conjunctiva that mainly showed cytoplasmatic localization. In view of these results, we hypothesized a possible gene expression modulator role played by Ang II in pterygium.</p><!--[if gte mso 9]><xml>
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topic |
Renin-angiotensin system (RAS), angiotensin II (Ang II), intracellular, immunohistochemistry. |
url |
http://www.ejh.it/index.php/ejh/article/view/2426 |
work_keys_str_mv |
AT pdemurtas angiotensiniiimmunohistochemicalstudyinsardinianpterygium AT mcorrias angiotensiniiimmunohistochemicalstudyinsardinianpterygium AT izucca angiotensiniiimmunohistochemicalstudyinsardinianpterygium AT cmaxia angiotensiniiimmunohistochemicalstudyinsardinianpterygium AT fpiras angiotensiniiimmunohistochemicalstudyinsardinianpterygium AT psirigu angiotensiniiimmunohistochemicalstudyinsardinianpterygium AT mtperra angiotensiniiimmunohistochemicalstudyinsardinianpterygium |
_version_ |
1724567546764460032 |
spelling |
doaj-92d88e85472644f1adf944e111371cc42020-11-25T03:32:33ZengPAGEPress PublicationsEuropean Journal of Histochemistry 1121-760X2038-83062014-07-0158310.4081/ejh.2014.24261412Angiotensin II: immunohistochemical study in Sardinian pterygiumP. Demurtas0M. Corrias1I. Zucca2C. Maxia3F. Piras4P. Sirigu5M.T. Perra6University of CagliariUniversity of CagliariUniversity of CagliariUniversity of CagliariUniversity of CagliariUniversity of CagliariUniversity of Cagliari<!--[if gte mso 9]><xml> <w:WordDocument> <w:View>Normal</w:View> <w:Zoom>0</w:Zoom> <w:HyphenationZone>14</w:HyphenationZone> <w:PunctuationKerning/> <w:ValidateAgainstSchemas/> <w:SaveIfXMLInvalid>false</w:SaveIfXMLInvalid> <w:IgnoreMixedContent>false</w:IgnoreMixedContent> <w:AlwaysShowPlaceholderText>false</w:AlwaysShowPlaceholderText> <w:Compatibility> <w:BreakWrappedTables/> <w:SnapToGridInCell/> <w:WrapTextWithPunct/> <w:UseAsianBreakRules/> <w:DontGrowAutofit/> </w:Compatibility> <w:BrowserLevel>MicrosoftInternetExplorer4</w:BrowserLevel> </w:WordDocument> </xml><![endif]--><!--[if gte mso 9]><xml> <w:WordDocument> <w:View>Normal</w:View> <w:Zoom>0</w:Zoom> <w:HyphenationZone>14</w:HyphenationZone> <w:PunctuationKerning/> <w:ValidateAgainstSchemas/> <w:SaveIfXMLInvalid>false</w:SaveIfXMLInvalid> <w:IgnoreMixedContent>false</w:IgnoreMixedContent> <w:AlwaysShowPlaceholderText>false</w:AlwaysShowPlaceholderText> <w:Compatibility> <w:BreakWrappedTables/> <w:SnapToGridInCell/> <w:WrapTextWithPunct/> <w:UseAsianBreakRules/> <w:DontGrowAutofit/> </w:Compatibility> <w:BrowserLevel>MicrosoftInternetExplorer4</w:BrowserLevel> </w:WordDocument> </xml><![endif]--><p>The angiotensin II (Ang II) is the principal effector peptide of the RAS system. It has a pleiotropic effect and, beside its <em>physiological</em> role, it has the property to stimulate angiogenesis and activate multiple signalling pathways related to cell proliferation. The purpose of the study was to determinate the Ang II expression and localization in Sardinian pterygium and normal conjunctiva by immunohistochemistry, and its possible involvement in the development and progression of the disease. Twenty-three pterygiums and eleven normal conjunctiva specimens obtained from Sardinian patients, were processed for paraffin embedding and assessed for the immunohistochemical revelation of Ang II. Significant Ang II expression was identified in pterygium and conjuntica. Particularly, thirteen pterygium specimens (n=13) displayed exclusively moderate to strong nuclear staining; some specimens (n=5) showed exclusively a moderate cytoplasmatic immunoreactivity, and few specimens (n=2) displayed moderate to strong immunoreactivity in both cytoplasm and nucleus. Statistical significance difference in respect of nuclear and cytoplasmatic localization was observed between normal conjunctiva and pterygium (P=0.038).The results showed a predominant intranuclear localization of Ang II in pterygium epithelial cells, in spite of conjunctiva that mainly showed cytoplasmatic localization. In view of these results, we hypothesized a possible gene expression modulator role played by Ang II in pterygium.</p><!--[if gte mso 9]><xml> <w:LatentStyles DefLockedState="false" LatentStyleCount="156"> </w:LatentStyles> </xml><![endif]--><!--[if gte mso 10]> <style> /* Style Definitions */ table.MsoNormalTable {mso-style-name:"Tabella normale"; mso-tstyle-rowband-size:0; mso-tstyle-colband-size:0; mso-style-noshow:yes; mso-style-parent:""; mso-padding-alt:0cm 5.4pt 0cm 5.4pt; mso-para-margin:0cm; mso-para-margin-bottom:.0001pt; mso-pagination:widow-orphan; font-size:10.0pt; font-family:"Times New Roman"; mso-ansi-language:#0400; mso-fareast-language:#0400; mso-bidi-language:#0400;} </style> <![endif]--><!--[if gte mso 9]><xml> <w:LatentStyles DefLockedState="false" LatentStyleCount="156"> </w:LatentStyles> </xml><![endif]--><!--[if gte mso 10]> <style> /* Style Definitions */ table.MsoNormalTable {mso-style-name:"Tabella normale"; mso-tstyle-rowband-size:0; mso-tstyle-colband-size:0; mso-style-noshow:yes; mso-style-parent:""; mso-padding-alt:0cm 5.4pt 0cm 5.4pt; mso-para-margin:0cm; mso-para-margin-bottom:.0001pt; mso-pagination:widow-orphan; font-size:10.0pt; font-family:"Times New Roman"; mso-ansi-language:#0400; mso-fareast-language:#0400; mso-bidi-language:#0400;} </style> <![endif]-->http://www.ejh.it/index.php/ejh/article/view/2426Renin-angiotensin system (RAS), angiotensin II (Ang II), intracellular, immunohistochemistry. |