Molecular Cloning and Expression Analysis of a MADS-Box Gene (<i>GbMADS2</i>) from <i>Ginkgo biloba
As a kind of transcription factors gene family, MADS-box genes play an important role in plant development processes. To find genes involved in the floral transition of <i>Ginkgo biloba</i>, a MADS-box gene, designated as <i style='mso-bidi-font-style:normal'>GbMADS2<s...
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doaj-930bb71329c8438ab5a32546c032e6782021-05-02T12:45:57ZengAcademicPresNotulae Botanicae Horti Agrobotanici Cluj-Napoca0255-965X1842-43092015-04-01431192410.15835/nbha.43.1.97608031Molecular Cloning and Expression Analysis of a MADS-Box Gene (<i>GbMADS2</i>) from <i>Ginkgo bilobaXiaohui WANG0Junhuan CHENG1Feng XU2Xingxiang LI3Weiwei ZHANG4Yongling LIAO5Shuiyuan CHENG6Xianbin LI7College of Horticulture and Gardening, Yangtze University, Jingzhou, 434023College of Horticulture and Gardening, Yangtze University, Jingzhou, 434023College of Horticulture and Gardening, Yangtze University, Jingzhou, 434023Medical School, Yangtze University, Jingzhou, 434023College of Horticulture and Gardening, Yangtze University, Jingzhou, 434023College of Forestry, Nanjing Forest University, Nanjing, 210037Wuhan Polytechnic University, Wuhan 430023Jingzhou Soil and Fertilizer Station, Jingzhou 434025As a kind of transcription factors gene family, MADS-box genes play an important role in plant development processes. To find genes involved in the floral transition of <i>Ginkgo biloba</i>, a MADS-box gene, designated as <i style='mso-bidi-font-style:normal'>GbMADS2<span style='mso-bidi-font-style: italic'>, </span></i>was cloned from <i>G. biloba </i>based on EST sequences by RT-PCR. Sequence analysis results showed that the cDNA sequence of <i style='mso-bidi-font-style:normal'>GbMADS2</i> contained a 663 bp length ORF encoding 221 amino acids protein, which displayed typical structure of plant MADS-box protein including MADS, I, and K domains and C terminus. The sequence of <span style='mso-bidi-font-style: italic'>GbMADS2 </span>protein was highly homologous to those of MADS-box proteins from other plant species with the highest homologous to <span style='mso-bidi-font-style:italic'>AGAMOUS<i> </i></span>(</span><span lang=EN-US style='font-size:10.0pt;font-family:"Garamond Premr Pro","serif"; mso-bidi-font-family:"Garamond Premr Pro Subh";color:black'>CyAG)<span style='mso-bidi-font-weight:bold'> from </span><i>Cycas revoluta</i><span style='mso-bidi-font-weight:bold'>. The phylogenetic tree analysis revealed that <i style='mso-bidi-font-style:normal'>GbMADS2 </i>belonged to <st1:stockticker w:st="on"><i>AGAM</i></st1:stockticker><i>OUS</i> clade genes. </span>Real-time PCR analysis indicated that expression levels of <i>GbMADS2</i> gene in female and male flower were significantly higher than those in root, stem, and leaves, and that <i>GbMADS2</i> expression level increased along with time of flower development. The spatial and time-course expression profile of <i>GbMADS2 </i>implied that <i>GbMADS2</i> might be involved in development of reproductive organs. The isolation and expression analysis of <i>GbMADS2</i> provided basis for further studying the molecular mechanism of flower development in <istyle='mso-bidi-font-style:normal'>G. biloba</i>.http://www.notulaebotanicae.ro/index.php/nbha/article/view/9760 |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Xiaohui WANG Junhuan CHENG Feng XU Xingxiang LI Weiwei ZHANG Yongling LIAO Shuiyuan CHENG Xianbin LI |
spellingShingle |
Xiaohui WANG Junhuan CHENG Feng XU Xingxiang LI Weiwei ZHANG Yongling LIAO Shuiyuan CHENG Xianbin LI Molecular Cloning and Expression Analysis of a MADS-Box Gene (<i>GbMADS2</i>) from <i>Ginkgo biloba Notulae Botanicae Horti Agrobotanici Cluj-Napoca |
author_facet |
Xiaohui WANG Junhuan CHENG Feng XU Xingxiang LI Weiwei ZHANG Yongling LIAO Shuiyuan CHENG Xianbin LI |
author_sort |
Xiaohui WANG |
title |
Molecular Cloning and Expression Analysis of a MADS-Box Gene (<i>GbMADS2</i>) from <i>Ginkgo biloba |
title_short |
Molecular Cloning and Expression Analysis of a MADS-Box Gene (<i>GbMADS2</i>) from <i>Ginkgo biloba |
title_full |
Molecular Cloning and Expression Analysis of a MADS-Box Gene (<i>GbMADS2</i>) from <i>Ginkgo biloba |
title_fullStr |
Molecular Cloning and Expression Analysis of a MADS-Box Gene (<i>GbMADS2</i>) from <i>Ginkgo biloba |
title_full_unstemmed |
Molecular Cloning and Expression Analysis of a MADS-Box Gene (<i>GbMADS2</i>) from <i>Ginkgo biloba |
title_sort |
molecular cloning and expression analysis of a mads-box gene (<i>gbmads2</i>) from <i>ginkgo biloba |
publisher |
AcademicPres |
series |
Notulae Botanicae Horti Agrobotanici Cluj-Napoca |
issn |
0255-965X 1842-4309 |
publishDate |
2015-04-01 |
description |
As a kind of transcription factors gene family, MADS-box genes play an important role in plant development processes. To find genes involved in the floral transition of <i>Ginkgo biloba</i>, a MADS-box gene, designated as <i style='mso-bidi-font-style:normal'>GbMADS2<span style='mso-bidi-font-style: italic'>, </span></i>was cloned from <i>G. biloba </i>based on EST sequences by RT-PCR. Sequence analysis results showed that the cDNA sequence of <i style='mso-bidi-font-style:normal'>GbMADS2</i> contained a 663 bp length ORF encoding 221 amino acids protein, which displayed typical structure of plant MADS-box protein including MADS, I, and K domains and C terminus. The sequence of <span style='mso-bidi-font-style: italic'>GbMADS2 </span>protein was highly homologous to those of MADS-box proteins from other plant species with the highest homologous to <span style='mso-bidi-font-style:italic'>AGAMOUS<i> </i></span>(</span><span lang=EN-US style='font-size:10.0pt;font-family:"Garamond Premr Pro","serif"; mso-bidi-font-family:"Garamond Premr Pro Subh";color:black'>CyAG)<span style='mso-bidi-font-weight:bold'> from </span><i>Cycas revoluta</i><span style='mso-bidi-font-weight:bold'>. The phylogenetic tree analysis revealed that <i style='mso-bidi-font-style:normal'>GbMADS2 </i>belonged to <st1:stockticker w:st="on"><i>AGAM</i></st1:stockticker><i>OUS</i> clade genes. </span>Real-time PCR analysis indicated that expression levels of <i>GbMADS2</i> gene in female and male flower were significantly higher than those in root, stem, and leaves, and that <i>GbMADS2</i> expression level increased along with time of flower development. The spatial and time-course expression profile of <i>GbMADS2 </i>implied that <i>GbMADS2</i> might be involved in development of reproductive organs. The isolation and expression analysis of <i>GbMADS2</i> provided basis for further studying the molecular mechanism of flower development in <istyle='mso-bidi-font-style:normal'>G. biloba</i>. |
url |
http://www.notulaebotanicae.ro/index.php/nbha/article/view/9760 |
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