Effects of quercetin on diabetic retinopathy and its association with NLRP3 inflammasome and autophagy
AIM: To investigate the effects of quercetin on diabetic retinopathy (DR) and its association with nucleotide-binding oligomerization domain-like receptors 3 (NLRP3) inflammasome and autophagy using retinal endothelial cell as an experimental model. METHODS: Human retinal microvascular endothelial c...
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doaj-9340fef9ca3d4a4a973587a168d1cdf72021-02-26T07:25:15ZengPress of International Journal of Ophthalmology (IJO PRESS)International Journal of Ophthalmology2222-39592227-48982021-01-01141424910.18240/ijo.2021.01.0620210106Effects of quercetin on diabetic retinopathy and its association with NLRP3 inflammasome and autophagyRong Li0Lin Chen1Guo-Min Yao2Hong-Lin Yan3Li Wang4Lin Chen. Department of Pharmacy, the First Affiliated Hospital of Xi'an Medical University, Xi'an 710077, Shaanxi Province, China. chenlin710077@sina.comDepartment of Pharmacy, the First Affiliated Hospital of Xi'an Medical University, Xi'an 710077, Shaanxi Province, ChinaDepartment of Ophthalmology, the First Affiliated Hospital of Xi'an Medical University, Xi'an 710077, Shaanxi Province, ChinaDepartment of Drug Clinical Trial Institution, the First Affiliated Hospital of Xi'an Medical University, Xi'an 710077, Shaanxi Province, ChinaDepartment of Scientific Research, the First Affiliated Hospital of Xi'an Medical University, Xi'an 710077, Shaanxi Province, ChinaAIM: To investigate the effects of quercetin on diabetic retinopathy (DR) and its association with nucleotide-binding oligomerization domain-like receptors 3 (NLRP3) inflammasome and autophagy using retinal endothelial cell as an experimental model. METHODS: Human retinal microvascular endothelial cells (HRMECs) were cultured in vitro and assigned into the control group, high-glucose (HG) group, and HG+different concentrations of quercetin groups. Cellular viability, migration, and tube formation in these groups was detected by MTT, transwell and matrigel assay, respectively. Expressions of NLRP3, apoptosis-associated speck-like protein (ASC), cysteiny aspartate-specific protease-1 (Caspase-1) as well as microtubule-related protein 1 light chain 3 (LC3) and Beclin-1 were detected by Western blotting. Expressions of IL-1β and IL-18 were detected by ELISA and cellular autophagy was detected by Cyto-ID® autophagy detection kit. RESULTS: Under an HG condition, the viability, migration, tube formation of HRMECs, and the protein expressions of NLRP3, ASC, Caspase-1, IL-1β, IL-18, LC3, and Beclin-1 as well as autophagy were all increased. Quercetin inhibited angiogenesis of HRMECs as well as the expressions of NLRP3, ASC, Caspase-1, IL-1β, IL-18, LC3, Beclin-1, and autophagy of HRMECs under a HG condition. The inhibitory effects of quercetin on angiogenesis, NLRP3 inflammasome and autophagy increased with the increase of its concentration. CONCLUSION: The therapeutic potential of quercetin in retinal neovascularization of DR, and inhibition of NLRP3 inflammasome and autophagy signaling pathway may be involved.http://ies.ijo.cn/en_publish/2021/1/20210106.pdfquercetinangiogenesisnlrp3autophagyhigh glucosediabetic retinopathy |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Rong Li Lin Chen Guo-Min Yao Hong-Lin Yan Li Wang |
spellingShingle |
Rong Li Lin Chen Guo-Min Yao Hong-Lin Yan Li Wang Effects of quercetin on diabetic retinopathy and its association with NLRP3 inflammasome and autophagy International Journal of Ophthalmology quercetin angiogenesis nlrp3 autophagy high glucose diabetic retinopathy |
author_facet |
Rong Li Lin Chen Guo-Min Yao Hong-Lin Yan Li Wang |
author_sort |
Rong Li |
title |
Effects of quercetin on diabetic retinopathy and its association with NLRP3 inflammasome and autophagy |
title_short |
Effects of quercetin on diabetic retinopathy and its association with NLRP3 inflammasome and autophagy |
title_full |
Effects of quercetin on diabetic retinopathy and its association with NLRP3 inflammasome and autophagy |
title_fullStr |
Effects of quercetin on diabetic retinopathy and its association with NLRP3 inflammasome and autophagy |
title_full_unstemmed |
Effects of quercetin on diabetic retinopathy and its association with NLRP3 inflammasome and autophagy |
title_sort |
effects of quercetin on diabetic retinopathy and its association with nlrp3 inflammasome and autophagy |
publisher |
Press of International Journal of Ophthalmology (IJO PRESS) |
series |
International Journal of Ophthalmology |
issn |
2222-3959 2227-4898 |
publishDate |
2021-01-01 |
description |
AIM: To investigate the effects of quercetin on diabetic retinopathy (DR) and its association with nucleotide-binding oligomerization domain-like receptors 3 (NLRP3) inflammasome and autophagy using retinal endothelial cell as an experimental model. METHODS: Human retinal microvascular endothelial cells (HRMECs) were cultured in vitro and assigned into the control group, high-glucose (HG) group, and HG+different concentrations of quercetin groups. Cellular viability, migration, and tube formation in these groups was detected by MTT, transwell and matrigel assay, respectively. Expressions of NLRP3, apoptosis-associated speck-like protein (ASC), cysteiny aspartate-specific protease-1 (Caspase-1) as well as microtubule-related protein 1 light chain 3 (LC3) and Beclin-1 were detected by Western blotting. Expressions of IL-1β and IL-18 were detected by ELISA and cellular autophagy was detected by Cyto-ID® autophagy detection kit. RESULTS: Under an HG condition, the viability, migration, tube formation of HRMECs, and the protein expressions of NLRP3, ASC, Caspase-1, IL-1β, IL-18, LC3, and Beclin-1 as well as autophagy were all increased. Quercetin inhibited angiogenesis of HRMECs as well as the expressions of NLRP3, ASC, Caspase-1, IL-1β, IL-18, LC3, Beclin-1, and autophagy of HRMECs under a HG condition. The inhibitory effects of quercetin on angiogenesis, NLRP3 inflammasome and autophagy increased with the increase of its concentration. CONCLUSION: The therapeutic potential of quercetin in retinal neovascularization of DR, and inhibition of NLRP3 inflammasome and autophagy signaling pathway may be involved. |
topic |
quercetin angiogenesis nlrp3 autophagy high glucose diabetic retinopathy |
url |
http://ies.ijo.cn/en_publish/2021/1/20210106.pdf |
work_keys_str_mv |
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