Isolation, characterization, and cross‐amplification of 20 microsatellite markers for Conospermum undulatum (Proteaceae)
Premise Recent habitat fragmentation is posing a risk to the wavy‐leaved smokebush, Conospermum undulatum (Proteaceae), a rare plant species endemic to southwestern Western Australia. Microsatellite markers are required to characterize the genetic diversity and structure of the species for conservat...
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doaj-9364fa87560449c0be36ac6c65ac5f182020-11-25T01:08:14ZengWileyApplications in Plant Sciences2168-04502019-08-0178n/an/a10.1002/aps3.11283Isolation, characterization, and cross‐amplification of 20 microsatellite markers for Conospermum undulatum (Proteaceae)Nicola Delnevo0Andrea Piotti1Eddie J. vanEtten2William D. Stock3Margaret Byrne4Centre for Ecosystem Management Edith Cowan University 270 Joondalup Drive Joondalup 6027 Western Australia AustraliaInstitute of Biosciences and BioResources (IBBR) National Research Council (CNR) 50019 Sesto Fiorentino (Firenze) ItalyCentre for Ecosystem Management Edith Cowan University 270 Joondalup Drive Joondalup 6027 Western Australia AustraliaCentre for Ecosystem Management Edith Cowan University 270 Joondalup Drive Joondalup 6027 Western Australia AustraliaBiodiversity and Conservation Science Department of Biodiversity Conservation and Attractions Bentley Delivery Centre Locked Bag 104 Bentley Western Australia 6983 AustraliaPremise Recent habitat fragmentation is posing a risk to the wavy‐leaved smokebush, Conospermum undulatum (Proteaceae), a rare plant species endemic to southwestern Western Australia. Microsatellite markers are required to characterize the genetic diversity and structure of the species for conservation purposes and to facilitate ecological studies. Methods and Results Illumina MiSeq high‐throughput sequencing was used to develop 20 novel microsatellite markers for C. undulatum. Polymorphism at each locus was assessed using 72 individuals from three natural populations. Nineteen markers were polymorphic, with the number of alleles per locus ranging from two to 21, and observed and expected heterozygosity ranging from 0.000 to 1.000 and 0.117 to 0.919, respectively. All markers successfully amplified in three congeneric species (C. stoechadis, C. canaliculatum and C. triplinervium). Conclusions The microsatellite markers will be useful for revealing patterns of genetic diversity, dispersal dynamics, and hybridization events for C. undulatum to inform future conservation efforts.https://doi.org/10.1002/aps3.11283AustraliaConospermum undulatumconservationhybridizationmicrosatellite primersProteaceae |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Nicola Delnevo Andrea Piotti Eddie J. vanEtten William D. Stock Margaret Byrne |
spellingShingle |
Nicola Delnevo Andrea Piotti Eddie J. vanEtten William D. Stock Margaret Byrne Isolation, characterization, and cross‐amplification of 20 microsatellite markers for Conospermum undulatum (Proteaceae) Applications in Plant Sciences Australia Conospermum undulatum conservation hybridization microsatellite primers Proteaceae |
author_facet |
Nicola Delnevo Andrea Piotti Eddie J. vanEtten William D. Stock Margaret Byrne |
author_sort |
Nicola Delnevo |
title |
Isolation, characterization, and cross‐amplification of 20 microsatellite markers for Conospermum undulatum (Proteaceae) |
title_short |
Isolation, characterization, and cross‐amplification of 20 microsatellite markers for Conospermum undulatum (Proteaceae) |
title_full |
Isolation, characterization, and cross‐amplification of 20 microsatellite markers for Conospermum undulatum (Proteaceae) |
title_fullStr |
Isolation, characterization, and cross‐amplification of 20 microsatellite markers for Conospermum undulatum (Proteaceae) |
title_full_unstemmed |
Isolation, characterization, and cross‐amplification of 20 microsatellite markers for Conospermum undulatum (Proteaceae) |
title_sort |
isolation, characterization, and cross‐amplification of 20 microsatellite markers for conospermum undulatum (proteaceae) |
publisher |
Wiley |
series |
Applications in Plant Sciences |
issn |
2168-0450 |
publishDate |
2019-08-01 |
description |
Premise Recent habitat fragmentation is posing a risk to the wavy‐leaved smokebush, Conospermum undulatum (Proteaceae), a rare plant species endemic to southwestern Western Australia. Microsatellite markers are required to characterize the genetic diversity and structure of the species for conservation purposes and to facilitate ecological studies. Methods and Results Illumina MiSeq high‐throughput sequencing was used to develop 20 novel microsatellite markers for C. undulatum. Polymorphism at each locus was assessed using 72 individuals from three natural populations. Nineteen markers were polymorphic, with the number of alleles per locus ranging from two to 21, and observed and expected heterozygosity ranging from 0.000 to 1.000 and 0.117 to 0.919, respectively. All markers successfully amplified in three congeneric species (C. stoechadis, C. canaliculatum and C. triplinervium). Conclusions The microsatellite markers will be useful for revealing patterns of genetic diversity, dispersal dynamics, and hybridization events for C. undulatum to inform future conservation efforts. |
topic |
Australia Conospermum undulatum conservation hybridization microsatellite primers Proteaceae |
url |
https://doi.org/10.1002/aps3.11283 |
work_keys_str_mv |
AT nicoladelnevo isolationcharacterizationandcrossamplificationof20microsatellitemarkersforconospermumundulatumproteaceae AT andreapiotti isolationcharacterizationandcrossamplificationof20microsatellitemarkersforconospermumundulatumproteaceae AT eddiejvanetten isolationcharacterizationandcrossamplificationof20microsatellitemarkersforconospermumundulatumproteaceae AT williamdstock isolationcharacterizationandcrossamplificationof20microsatellitemarkersforconospermumundulatumproteaceae AT margaretbyrne isolationcharacterizationandcrossamplificationof20microsatellitemarkersforconospermumundulatumproteaceae |
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