N-Linked Glycopeptide Identification Based on Open Mass Spectral Library Search
Confident characterization of intact glycopeptides is a challenging task in mass spectrometry-based glycoproteomics due to microheterogeneity of glycosylation, complexity of glycans, and insufficient fragmentation of peptide bones. Open mass spectral library search is a promising computational appro...
Main Authors: | , , , , , , |
---|---|
Format: | Article |
Language: | English |
Published: |
Hindawi Limited
2018-01-01
|
Series: | BioMed Research International |
Online Access: | http://dx.doi.org/10.1155/2018/1564136 |
id |
doaj-9367f3fc4458411bb04869919850e5de |
---|---|
record_format |
Article |
spelling |
doaj-9367f3fc4458411bb04869919850e5de2020-11-24T23:23:49ZengHindawi LimitedBioMed Research International2314-61332314-61412018-01-01201810.1155/2018/15641361564136N-Linked Glycopeptide Identification Based on Open Mass Spectral Library SearchZhiwu An0Qingbo Shu1Hao Lv2Lian Shu3Jifeng Wang4Fuquan Yang5Yan Fu6National Center for Mathematics and Interdisciplinary Sciences, Key Laboratory of Random Complex Structures and Data Science, Academy of Mathematics and Systems Science, Chinese Academy of Sciences, Beijing 100101, ChinaUniversity of Chinese Academy of Sciences, Beijing 100049, ChinaNational Center for Mathematics and Interdisciplinary Sciences, Key Laboratory of Random Complex Structures and Data Science, Academy of Mathematics and Systems Science, Chinese Academy of Sciences, Beijing 100101, ChinaUniversity of Chinese Academy of Sciences, Beijing 100049, ChinaLaboratory of Proteomics, Institute of Biophysics, Chinese Academy of Sciences, Beijing 100101, ChinaUniversity of Chinese Academy of Sciences, Beijing 100049, ChinaNational Center for Mathematics and Interdisciplinary Sciences, Key Laboratory of Random Complex Structures and Data Science, Academy of Mathematics and Systems Science, Chinese Academy of Sciences, Beijing 100101, ChinaConfident characterization of intact glycopeptides is a challenging task in mass spectrometry-based glycoproteomics due to microheterogeneity of glycosylation, complexity of glycans, and insufficient fragmentation of peptide bones. Open mass spectral library search is a promising computational approach to peptide identification, but its potential in the identification of glycopeptides has not been fully explored. Here we present pMatchGlyco, a new spectral library search tool for intact N-linked glycopeptide identification using high-energy collisional dissociation (HCD) tandem mass spectrometry (MS/MS) data. In pMatchGlyco, (1) MS/MS spectra of deglycopeptides are used to create spectral library, (2) MS/MS spectra of glycopeptides are matched to the spectra in library in an open (precursor tolerant) manner and the glycans are inferred, and (3) a false discovery rate is estimated for top-scored matches above a threshold. The efficiency and reliability of pMatchGlyco were demonstrated on a data set of mixture sample of six standard glycoproteins and a complex glycoprotein data set generated from human cancer cell line OVCAR3.http://dx.doi.org/10.1155/2018/1564136 |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Zhiwu An Qingbo Shu Hao Lv Lian Shu Jifeng Wang Fuquan Yang Yan Fu |
spellingShingle |
Zhiwu An Qingbo Shu Hao Lv Lian Shu Jifeng Wang Fuquan Yang Yan Fu N-Linked Glycopeptide Identification Based on Open Mass Spectral Library Search BioMed Research International |
author_facet |
Zhiwu An Qingbo Shu Hao Lv Lian Shu Jifeng Wang Fuquan Yang Yan Fu |
author_sort |
Zhiwu An |
title |
N-Linked Glycopeptide Identification Based on Open Mass Spectral Library Search |
title_short |
N-Linked Glycopeptide Identification Based on Open Mass Spectral Library Search |
title_full |
N-Linked Glycopeptide Identification Based on Open Mass Spectral Library Search |
title_fullStr |
N-Linked Glycopeptide Identification Based on Open Mass Spectral Library Search |
title_full_unstemmed |
N-Linked Glycopeptide Identification Based on Open Mass Spectral Library Search |
title_sort |
n-linked glycopeptide identification based on open mass spectral library search |
publisher |
Hindawi Limited |
series |
BioMed Research International |
issn |
2314-6133 2314-6141 |
publishDate |
2018-01-01 |
description |
Confident characterization of intact glycopeptides is a challenging task in mass spectrometry-based glycoproteomics due to microheterogeneity of glycosylation, complexity of glycans, and insufficient fragmentation of peptide bones. Open mass spectral library search is a promising computational approach to peptide identification, but its potential in the identification of glycopeptides has not been fully explored. Here we present pMatchGlyco, a new spectral library search tool for intact N-linked glycopeptide identification using high-energy collisional dissociation (HCD) tandem mass spectrometry (MS/MS) data. In pMatchGlyco, (1) MS/MS spectra of deglycopeptides are used to create spectral library, (2) MS/MS spectra of glycopeptides are matched to the spectra in library in an open (precursor tolerant) manner and the glycans are inferred, and (3) a false discovery rate is estimated for top-scored matches above a threshold. The efficiency and reliability of pMatchGlyco were demonstrated on a data set of mixture sample of six standard glycoproteins and a complex glycoprotein data set generated from human cancer cell line OVCAR3. |
url |
http://dx.doi.org/10.1155/2018/1564136 |
work_keys_str_mv |
AT zhiwuan nlinkedglycopeptideidentificationbasedonopenmassspectrallibrarysearch AT qingboshu nlinkedglycopeptideidentificationbasedonopenmassspectrallibrarysearch AT haolv nlinkedglycopeptideidentificationbasedonopenmassspectrallibrarysearch AT lianshu nlinkedglycopeptideidentificationbasedonopenmassspectrallibrarysearch AT jifengwang nlinkedglycopeptideidentificationbasedonopenmassspectrallibrarysearch AT fuquanyang nlinkedglycopeptideidentificationbasedonopenmassspectrallibrarysearch AT yanfu nlinkedglycopeptideidentificationbasedonopenmassspectrallibrarysearch |
_version_ |
1725563472621600768 |