The protective effect of Dendrobium officinale polysaccharides on photoaging fibroblasts by scavenging reactive oxygen species and promoting the expression of TGF-β1

• Main Authors: Rui Tang, Qia-Qia Li, Di Wang, Jing Chen, Jin-Hua Huang, Qing-Hai Zeng
• Format: Article
• Language: English
• Published: Hong Kong Gold Orchid Science and Technology Co., Limited 2018-05-01
• Series: Traditional Medicine Research
• Subjects: Dendrobium officinale polysaccharides; Photoaging fibroblasts; ROS; MMP-1; TGF-β1; Collagen protein
• Online Access: http://www.tmrjournal.com/EN/abstract/abstract245.shtml

The purpose of this study was to explore the protective effect of Dendrobium officinale polysaccharides (DOP) on photoaging human skin fibroblasts and its specific mechanism of action. The photoaging fibroblast model was established by ultraviolet B (UVB) irradiation. The toxic effects of different concentrations of DOP were detected using MTT. Senescent cells were detected using a β-galactosidase kit. Reactive oxygen species (ROS) in cells were detected using a flow cytometer. The expression of matrix metalloproteinase-1 (MMP-1), type I collagen C-terminal peptide (CICP), and transforming growth factor β-1 (TGF-β1) in spent culture medium was detected by ELISA. The results showed that the low concentration of DOP (20, 40, 80 μg/mL) had no cytotoxicity on fibroblasts. After 60 mJ/cm2 UVB irradiation, the number of aging β-gal-positive cells increased, the levels of CICP and TGF-β1 in spent culture medium decreased, while the levels of MMP-1 and ROS increased. After administration of DOP on photoaging fibroblasts, the number of aging β-gal-positive cells decreased, the levels of ROS and MMP-1 decreased, and the levels of TGF-β1 and CICP increased. This experiment suggests that DOP has the effect of removing ROS induced by UVB, regulating the balance of collagen production and degradation, and protecting photoaging human skin fibroblasts.