Phenotype, Function, and Mobilization of 6-Sulfo LacNAc-Expressing Monocytes in Atopic Dermatitis
Mononuclear phagocytes (MPs) are important immune regulatory cells in atopic dermatitis (AD). We previously identified 6-sulfo LacNAc-expressing monocytes (slanMo) as TNF-α- and IL-23-producing cells in psoriatic skin lesions and as inducers of IFN-γ-, IL-17-, and IL-22-producing T cells. These cyto...
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doaj-93a2ba30cab24fbb8c89229bbe4c4a8c2020-11-25T00:47:39ZengFrontiers Media S.A.Frontiers in Immunology1664-32242018-06-01910.3389/fimmu.2018.01352361601Phenotype, Function, and Mobilization of 6-Sulfo LacNAc-Expressing Monocytes in Atopic DermatitisWojciech Baran0Stephanie Oehrl1Fareed Ahmad2Thomas Döbel3Christina Alt4Angelika Buske-Kirschbaum5Marc Schmitz6Marc Schmitz7Marc Schmitz8Marc Schmitz9Marc Schmitz10Knut Schäkel11Department of Dermatology, Venerology and Allergology, Wroclaw Medical University, Wroclaw, PolandDepartment of Dermatology, Medical Faculty, University of Heidelberg, Heidelberg, GermanyDepartment of Dermatology, Medical Faculty, University of Heidelberg, Heidelberg, GermanyDepartment of Dermatology, Medical Faculty, University of Heidelberg, Heidelberg, GermanyDepartment of Dermatology, Medical Faculty, University of Heidelberg, Heidelberg, GermanyDepartment of Biopsycology, Technical University of Dresden, Dresden, GermanyInstitute of Immunology, Medical Faculty, Technical University of Dresden, Dresden, GermanyNational Center for Tumor Diseases, University Hospital Carl Gustav Carus, Technical University of Dresden, Dresden, GermanyGerman Cancer Consortium (DKTK), Dresden, GermanyGerman Cancer Research Center (DKFZ), Heidelberg, GermanyCenter for Regenerative Therapies Dresden (CRTD), Medical Faculty, Technical University of Dresden, Dresden, GermanyDepartment of Dermatology, Medical Faculty, University of Heidelberg, Heidelberg, GermanyMononuclear phagocytes (MPs) are important immune regulatory cells in atopic dermatitis (AD). We previously identified 6-sulfo LacNAc-expressing monocytes (slanMo) as TNF-α- and IL-23-producing cells in psoriatic skin lesions and as inducers of IFN-γ-, IL-17-, and IL-22-producing T cells. These cytokines are also upregulated in AD and normalize with treatment, as recently shown for dupilumab-treated patients. We here asked for the role of slanMo in AD. Increased numbers of slanMo were found in AD skin lesions. In difference to other MPs in AD, slanMo lacked expression of FcɛRI, CD1a, CD14, and CD163. slanMo from blood of patients with AD expressed increased levels of CD86 and produced IL-12 and TNF-α at higher amounts than CD14+ monocytes and myeloid dendritic cells. While CD14+ monocytes from patients with AD revealed a reduced IL-12 production, we observed no difference in the cytokine production comparing slanMo in AD and healthy controls. Interestingly, experimentally induced mental stress, a common trigger of flares in patients with AD, rapidly mobilized slanMo which retained their high TNF-α-producing capacity. This study identifies slanMo as a distinct population of inflammatory cells in skin lesions and as proinflammatory blood cells in patients with AD. slanMo may, therefore, represent a potent future target for treatment of AD.https://www.frontiersin.org/article/10.3389/fimmu.2018.01352/fullatopic dermatitisdendritic cells6-sulfo LacNAc-expressing monocytesimmunologyinflammationIL-12 |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Wojciech Baran Stephanie Oehrl Fareed Ahmad Thomas Döbel Christina Alt Angelika Buske-Kirschbaum Marc Schmitz Marc Schmitz Marc Schmitz Marc Schmitz Marc Schmitz Knut Schäkel |
spellingShingle |
Wojciech Baran Stephanie Oehrl Fareed Ahmad Thomas Döbel Christina Alt Angelika Buske-Kirschbaum Marc Schmitz Marc Schmitz Marc Schmitz Marc Schmitz Marc Schmitz Knut Schäkel Phenotype, Function, and Mobilization of 6-Sulfo LacNAc-Expressing Monocytes in Atopic Dermatitis Frontiers in Immunology atopic dermatitis dendritic cells 6-sulfo LacNAc-expressing monocytes immunology inflammation IL-12 |
author_facet |
Wojciech Baran Stephanie Oehrl Fareed Ahmad Thomas Döbel Christina Alt Angelika Buske-Kirschbaum Marc Schmitz Marc Schmitz Marc Schmitz Marc Schmitz Marc Schmitz Knut Schäkel |
author_sort |
Wojciech Baran |
title |
Phenotype, Function, and Mobilization of 6-Sulfo LacNAc-Expressing Monocytes in Atopic Dermatitis |
title_short |
Phenotype, Function, and Mobilization of 6-Sulfo LacNAc-Expressing Monocytes in Atopic Dermatitis |
title_full |
Phenotype, Function, and Mobilization of 6-Sulfo LacNAc-Expressing Monocytes in Atopic Dermatitis |
title_fullStr |
Phenotype, Function, and Mobilization of 6-Sulfo LacNAc-Expressing Monocytes in Atopic Dermatitis |
title_full_unstemmed |
Phenotype, Function, and Mobilization of 6-Sulfo LacNAc-Expressing Monocytes in Atopic Dermatitis |
title_sort |
phenotype, function, and mobilization of 6-sulfo lacnac-expressing monocytes in atopic dermatitis |
publisher |
Frontiers Media S.A. |
series |
Frontiers in Immunology |
issn |
1664-3224 |
publishDate |
2018-06-01 |
description |
Mononuclear phagocytes (MPs) are important immune regulatory cells in atopic dermatitis (AD). We previously identified 6-sulfo LacNAc-expressing monocytes (slanMo) as TNF-α- and IL-23-producing cells in psoriatic skin lesions and as inducers of IFN-γ-, IL-17-, and IL-22-producing T cells. These cytokines are also upregulated in AD and normalize with treatment, as recently shown for dupilumab-treated patients. We here asked for the role of slanMo in AD. Increased numbers of slanMo were found in AD skin lesions. In difference to other MPs in AD, slanMo lacked expression of FcɛRI, CD1a, CD14, and CD163. slanMo from blood of patients with AD expressed increased levels of CD86 and produced IL-12 and TNF-α at higher amounts than CD14+ monocytes and myeloid dendritic cells. While CD14+ monocytes from patients with AD revealed a reduced IL-12 production, we observed no difference in the cytokine production comparing slanMo in AD and healthy controls. Interestingly, experimentally induced mental stress, a common trigger of flares in patients with AD, rapidly mobilized slanMo which retained their high TNF-α-producing capacity. This study identifies slanMo as a distinct population of inflammatory cells in skin lesions and as proinflammatory blood cells in patients with AD. slanMo may, therefore, represent a potent future target for treatment of AD. |
topic |
atopic dermatitis dendritic cells 6-sulfo LacNAc-expressing monocytes immunology inflammation IL-12 |
url |
https://www.frontiersin.org/article/10.3389/fimmu.2018.01352/full |
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