Purification of Egg White Lysozyme from Indonesian Kampung Chicken and Ducks
Egg white lysozyme (EWL) has considerably a wide functional protein exhibiting antibacterial activity mainly against Gram-positive bacteria. The EWL is widely applied in food industry and is considerably safe. Despite its high potency, EWL of Indonesian poultry has never been studied and exploited....
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Bogor Agricultural University
2015-04-01
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doaj-93b38f65552649db93bc07f846404ca72020-11-24T22:43:16ZengBogor Agricultural UniversityMedia Peternakan0126-04722087-46342015-04-01381182610.5398/medpet.2015.38.1.18Purification of Egg White Lysozyme from Indonesian Kampung Chicken and DucksZ. Wulandari 0D. Fardiaz 1C. Budiman2T. Suryati 3D. Herawati 4Department of Animal Production and Technology, Faculty of Animal Science, Bogor Agricultural University, IndonesiaDepartment of Food Science and Technology, Faculty of Agriculture and Technology, Bogor Agricultural University, IndonesiaDepartment of Animal Production and Technology, Faculty of Animal Science, Bogor Agricultural University, IndonesiaDepartment of Animal Production and Technology, Faculty of Animal Science, Bogor Agricultural University, IndonesiaDepartment of Food Science and Technology, Faculty of Agriculture and Technology, Bogor Agricultural University, IndonesiaEgg white lysozyme (EWL) has considerably a wide functional protein exhibiting antibacterial activity mainly against Gram-positive bacteria. The EWL is widely applied in food industry and is considerably safe. Despite its high potency, EWL of Indonesian poultry has never been studied and exploited. This study was aimed to purify EWL from two Indonesian poultry: kampung chicken and Cihateup duck, and compared to egg of commercial laying hens. The eggs in this study were obtained from field laboratory of Faculty of Animal Science, Bogor Agricultural University (IPB) and classified in AA quality based on the interior quality. First attempt to purify the EWL was performed by using ethanol precipitation yielding purified EWL which was still contaminated by other proteins, hence designated as partially purified EWL. Final concentrations of partially purified EWL of kampung chicken, commercial laying hens, and Cihateup duck were about 5800, 5400, and 5500 μg/mL, respectively. To confirm whether the use of ethanol in the purification affecting EWL antibacterial activities, the activities were examined against Staphylococcus aureus. It demonstrated that the partially purified EWL exhibited ability to inhibit S. aureus at 6 and 26 h suggesting that the method was feasible as it did not interfere EWL antibacterial activities. Yet, based on SDS-Page, purity was the issue in ethanol precipitation method. Further attempt using ion exchange chromatography at pH 10 successfully purified lysozyme as indicated by a single band corresponding to lysozyme size (~14 kD) free from bands of other proteins. Altogether, a single step of ion exchange chromatography is sufficient and promising to isolate EWL from Indonesian poultry for various industrial purposes.http://medpet.journal.ipb.ac.id/index.php/mediapeternakan/article/view/8992Indonesian poultrylysozymeeggkampung chickenCihateup duck |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Z. Wulandari D. Fardiaz C. Budiman T. Suryati D. Herawati |
spellingShingle |
Z. Wulandari D. Fardiaz C. Budiman T. Suryati D. Herawati Purification of Egg White Lysozyme from Indonesian Kampung Chicken and Ducks Media Peternakan Indonesian poultry lysozyme egg kampung chicken Cihateup duck |
author_facet |
Z. Wulandari D. Fardiaz C. Budiman T. Suryati D. Herawati |
author_sort |
Z. Wulandari |
title |
Purification of Egg White Lysozyme from Indonesian Kampung Chicken and Ducks |
title_short |
Purification of Egg White Lysozyme from Indonesian Kampung Chicken and Ducks |
title_full |
Purification of Egg White Lysozyme from Indonesian Kampung Chicken and Ducks |
title_fullStr |
Purification of Egg White Lysozyme from Indonesian Kampung Chicken and Ducks |
title_full_unstemmed |
Purification of Egg White Lysozyme from Indonesian Kampung Chicken and Ducks |
title_sort |
purification of egg white lysozyme from indonesian kampung chicken and ducks |
publisher |
Bogor Agricultural University |
series |
Media Peternakan |
issn |
0126-0472 2087-4634 |
publishDate |
2015-04-01 |
description |
Egg white lysozyme (EWL) has considerably a wide functional protein exhibiting antibacterial activity mainly against Gram-positive bacteria. The EWL is widely applied in food industry and is considerably safe. Despite its high potency, EWL of Indonesian poultry has never been studied and exploited. This study was aimed to purify EWL from two Indonesian poultry: kampung chicken and Cihateup duck, and compared to egg of commercial laying hens. The eggs in this study were obtained from field laboratory of Faculty of Animal Science, Bogor Agricultural University (IPB) and classified in AA quality based on the interior quality. First attempt to purify the EWL was performed by using ethanol precipitation yielding purified EWL which was still contaminated by other proteins, hence designated as partially purified EWL. Final concentrations of partially purified EWL of kampung chicken, commercial laying hens, and Cihateup duck were about 5800, 5400, and 5500 μg/mL, respectively. To confirm whether the use of ethanol in the purification affecting EWL antibacterial activities, the activities were examined against Staphylococcus aureus. It demonstrated that the partially purified EWL exhibited ability to inhibit S. aureus at 6 and 26 h suggesting that the method was feasible as it did not interfere EWL antibacterial activities. Yet, based on SDS-Page, purity was the issue in ethanol precipitation method. Further attempt using ion exchange chromatography at pH 10 successfully purified lysozyme as indicated by a single band corresponding to lysozyme size (~14 kD) free from bands of other proteins. Altogether, a single step of ion exchange chromatography is sufficient and promising to isolate EWL from Indonesian poultry for various industrial purposes. |
topic |
Indonesian poultry lysozyme egg kampung chicken Cihateup duck |
url |
http://medpet.journal.ipb.ac.id/index.php/mediapeternakan/article/view/8992 |
work_keys_str_mv |
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