Comparison of two culture techniques used to detect environmental contamination with <i>Salmonella enterica</i> in a large-animal hospital
Salmonellosis is a common healthcare-associated infection in large-animal hospitals, and surveillance for Salmonella is an integral part of comprehensive infection control programmes in populations at risk. The present study compares the effectiveness of two culture techniques for recovery of Salmon...
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doaj-93c252e489f24ab18838816b08ec7f272020-11-24T22:27:40ZengAOSISJournal of the South African Veterinary Association1019-91282224-94352015-08-01861e1e510.4102/jsava.v86i1.12921014Comparison of two culture techniques used to detect environmental contamination with <i>Salmonella enterica</i> in a large-animal hospitalCatriona H. Lyle0Cornelius H. Annandale1Johan Gouws2Paul S. Morley3Department of Companion Animal Clinical Studies, University of PretoriaDepartment of Production Animal Studies, University of PretoriaDepartment of Veterinary Tropical Diseases, University of PretoriaDepartment of Companion Animal Clinical Studies, University of Pretoria, South Africa; College of Veterinary Medicine and Biomedical Sciences, Colorado State University, United StatesSalmonellosis is a common healthcare-associated infection in large-animal hospitals, and surveillance for Salmonella is an integral part of comprehensive infection control programmes in populations at risk. The present study compares the effectiveness of two culture techniques for recovery of Salmonella from environmental samples obtained in a large-animal referral veterinary hospital during a Salmonella outbreak. Environmental samples were collected using household cleaning cloths that were incubated overnight in buffered peptone water (BPW). Aliquots of BPW were then processed using two different selective enrichment and culture techniques. In the first technique (TBG-RV-XLT4) samples were incubated at 43 °C in tetrathionate broth and then Rappaport-Vassiliadis broth before plating on XLT4 agar. The second technique (SEL-XLD) involved incubation at 37 °C in selenite broth before plating on XLD agar. Salmonella was recovered from 49.7% (73/147) of samples using the TBG-RV-XLT4 technique, but only 10.2% (15/147) of samples using the SEL-XLD method. Fourteen samples (9.5%) were culture-positive using both methods, and 73 (49.7%) were culture-negative using both techniques. There were discordant results for 60 samples, including 59 that were only culture-positive using the TBG-RV-XLT4 method, and one sample that was only culturepositive using the SEL-XLD method. Salmonella was much more likely to be recovered using the TBG-RV-XLT4 method, and there appeared to be five times more false-negative results using the SEL-XLD technique. Environmental contamination with Salmonella may be underestimated by certain culture techniques, which may impair efforts to control spread in veterinary hospitals.https://jsava.co.za/index.php/jsava/article/view/1292horse, nosocomial infection, infection control, surveillance |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Catriona H. Lyle Cornelius H. Annandale Johan Gouws Paul S. Morley |
spellingShingle |
Catriona H. Lyle Cornelius H. Annandale Johan Gouws Paul S. Morley Comparison of two culture techniques used to detect environmental contamination with <i>Salmonella enterica</i> in a large-animal hospital Journal of the South African Veterinary Association horse, nosocomial infection, infection control, surveillance |
author_facet |
Catriona H. Lyle Cornelius H. Annandale Johan Gouws Paul S. Morley |
author_sort |
Catriona H. Lyle |
title |
Comparison of two culture techniques used to detect environmental contamination with <i>Salmonella enterica</i> in a large-animal hospital |
title_short |
Comparison of two culture techniques used to detect environmental contamination with <i>Salmonella enterica</i> in a large-animal hospital |
title_full |
Comparison of two culture techniques used to detect environmental contamination with <i>Salmonella enterica</i> in a large-animal hospital |
title_fullStr |
Comparison of two culture techniques used to detect environmental contamination with <i>Salmonella enterica</i> in a large-animal hospital |
title_full_unstemmed |
Comparison of two culture techniques used to detect environmental contamination with <i>Salmonella enterica</i> in a large-animal hospital |
title_sort |
comparison of two culture techniques used to detect environmental contamination with <i>salmonella enterica</i> in a large-animal hospital |
publisher |
AOSIS |
series |
Journal of the South African Veterinary Association |
issn |
1019-9128 2224-9435 |
publishDate |
2015-08-01 |
description |
Salmonellosis is a common healthcare-associated infection in large-animal hospitals, and surveillance for Salmonella is an integral part of comprehensive infection control programmes in populations at risk. The present study compares the effectiveness of two culture techniques for recovery of Salmonella from environmental samples obtained in a large-animal referral veterinary hospital during a Salmonella outbreak. Environmental samples were collected using household cleaning cloths that were incubated overnight in buffered peptone water (BPW). Aliquots of BPW were then processed using two different selective enrichment and culture techniques. In the first technique (TBG-RV-XLT4) samples were incubated at 43 °C in tetrathionate broth and then Rappaport-Vassiliadis broth before plating on XLT4 agar. The second technique (SEL-XLD) involved incubation at 37 °C in selenite broth before plating on XLD agar. Salmonella was recovered from 49.7% (73/147) of samples using the TBG-RV-XLT4 technique, but only 10.2% (15/147) of samples using the SEL-XLD method. Fourteen samples (9.5%) were culture-positive using both methods, and 73 (49.7%) were culture-negative using both techniques. There were discordant results for 60 samples, including 59 that were only culture-positive using the TBG-RV-XLT4 method, and one sample that was only culturepositive using the SEL-XLD method. Salmonella was much more likely to be recovered using the TBG-RV-XLT4 method, and there appeared to be five times more false-negative results using the SEL-XLD technique. Environmental contamination with Salmonella may be underestimated by certain culture techniques, which may impair efforts to control spread in veterinary hospitals. |
topic |
horse, nosocomial infection, infection control, surveillance |
url |
https://jsava.co.za/index.php/jsava/article/view/1292 |
work_keys_str_mv |
AT catrionahlyle comparisonoftwoculturetechniquesusedtodetectenvironmentalcontaminationwithisalmonellaentericaiinalargeanimalhospital AT corneliushannandale comparisonoftwoculturetechniquesusedtodetectenvironmentalcontaminationwithisalmonellaentericaiinalargeanimalhospital AT johangouws comparisonoftwoculturetechniquesusedtodetectenvironmentalcontaminationwithisalmonellaentericaiinalargeanimalhospital AT paulsmorley comparisonoftwoculturetechniquesusedtodetectenvironmentalcontaminationwithisalmonellaentericaiinalargeanimalhospital |
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