Comparison of Anti-Viral Activity of Frog Skin Anti-Microbial Peptides Temporin-Sha and [K3]SHa to LL-37 and Temporin-Tb against Herpes Simplex Virus Type 1
Temporins are anti-microbial peptides synthesized in the skin of frogs of the Ranidae family. The few studies to date that have examined their anti-viral properties have shown that they have potential as anti-viral therapies. In this work, we evaluated the anti-herpes simplex virus type 1 (HSV-1) ac...
Main Authors: | , , , , , , , , |
---|---|
Format: | Article |
Language: | English |
Published: |
MDPI AG
2019-01-01
|
Series: | Viruses |
Subjects: | |
Online Access: | http://www.mdpi.com/1999-4915/11/1/77 |
id |
doaj-93cae83a6c264f9382210f1d5503cace |
---|---|
record_format |
Article |
spelling |
doaj-93cae83a6c264f9382210f1d5503cace2020-11-25T00:17:33ZengMDPI AGViruses1999-49152019-01-011117710.3390/v11010077v11010077Comparison of Anti-Viral Activity of Frog Skin Anti-Microbial Peptides Temporin-Sha and [K3]SHa to LL-37 and Temporin-Tb against Herpes Simplex Virus Type 1Maëva Roy0Lucie Lebeau1Céline Chessa2Alexia Damour3Ali Ladram4Bruno Oury5David Boutolleau6Charles Bodet7Nicolas Lévêque8Laboratoire Inflammation, Tissus Epithéliaux et Cytokines, LITEC EA 4331, Université de Poitiers, 86000 Poitiers, FranceLaboratoire de Virologie et Mycobactériologie, CHU de Poitiers, 86000 Poitiers, FranceLaboratoire Inflammation, Tissus Epithéliaux et Cytokines, LITEC EA 4331, Université de Poitiers, 86000 Poitiers, FranceLaboratoire Inflammation, Tissus Epithéliaux et Cytokines, LITEC EA 4331, Université de Poitiers, 86000 Poitiers, FranceSorbonne Université, CNRS, Institut de Biologie Paris-Seine, IBPS, BIOSIPE, 75252 Paris, FranceInstitut de Recherche pour le Développement (IRD), UMR 224 IRD-CNRS-Univ Montpellier 1 et 2 Maladies infectieuses et Vecteurs: écologie, génétique, évolution et contrôle (MiVegec), 34394 Montpellier, FranceSorbonne Universités, Centre d’Immunologie et des Maladies Infectieuses (CIMI-Paris), INSERM U1135, Eq1, 75013 Paris, FranceLaboratoire Inflammation, Tissus Epithéliaux et Cytokines, LITEC EA 4331, Université de Poitiers, 86000 Poitiers, FranceLaboratoire Inflammation, Tissus Epithéliaux et Cytokines, LITEC EA 4331, Université de Poitiers, 86000 Poitiers, FranceTemporins are anti-microbial peptides synthesized in the skin of frogs of the Ranidae family. The few studies to date that have examined their anti-viral properties have shown that they have potential as anti-viral therapies. In this work, we evaluated the anti-herpes simplex virus type 1 (HSV-1) activity of the temporin-SHa (SHa) and its synthetic analog [K3]SHa. Human cathelicidin LL-37 and temporin-Tb (Tb), previously demonstrated to have anti-HSV-1 properties, were used as positive controls. We observed that SHa and [K3]SHa significantly inhibit HSV-1 replication in human primary keratinocytes when used at micromolar concentrations. This anti-viral activity was equivalent to that of Tb, but lower than that of LL-37. Transcriptomic analyses revealed that SHa did not act through the modulation of the cell innate immune response, but rather, displayed virucidal properties by reducing infectious titer of HSV-1 in suspension. In contrast, pre-incubation of the virus with LL-37 suggests that this peptide does not act directly on the viral particle at non-cytotoxic concentrations tested. The anti-HSV-1 activity of LL-37 appears to be due to the potentiation of cellular anti-viral defenses through the induction of interferon stimulated gene expression in infected primary keratinocytes. This study demonstrated that SHa and [K3]SHa, in addition to their previously reported antibacterial and antiparasitic activities, are direct-acting anti-HSV-1 peptides. Importantly, this study extends the little studied anti-viral attributes of frog temporins and offers perspectives for the development of new anti-HSV-1 therapies.http://www.mdpi.com/1999-4915/11/1/77temporinSHa[K3]SHaTbLL-37herpes simplex virus type 1keratinocytecytotoxicityanti-viralimmunomodulation |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Maëva Roy Lucie Lebeau Céline Chessa Alexia Damour Ali Ladram Bruno Oury David Boutolleau Charles Bodet Nicolas Lévêque |
spellingShingle |
Maëva Roy Lucie Lebeau Céline Chessa Alexia Damour Ali Ladram Bruno Oury David Boutolleau Charles Bodet Nicolas Lévêque Comparison of Anti-Viral Activity of Frog Skin Anti-Microbial Peptides Temporin-Sha and [K3]SHa to LL-37 and Temporin-Tb against Herpes Simplex Virus Type 1 Viruses temporin SHa [K3]SHa Tb LL-37 herpes simplex virus type 1 keratinocyte cytotoxicity anti-viral immunomodulation |
author_facet |
Maëva Roy Lucie Lebeau Céline Chessa Alexia Damour Ali Ladram Bruno Oury David Boutolleau Charles Bodet Nicolas Lévêque |
author_sort |
Maëva Roy |
title |
Comparison of Anti-Viral Activity of Frog Skin Anti-Microbial Peptides Temporin-Sha and [K3]SHa to LL-37 and Temporin-Tb against Herpes Simplex Virus Type 1 |
title_short |
Comparison of Anti-Viral Activity of Frog Skin Anti-Microbial Peptides Temporin-Sha and [K3]SHa to LL-37 and Temporin-Tb against Herpes Simplex Virus Type 1 |
title_full |
Comparison of Anti-Viral Activity of Frog Skin Anti-Microbial Peptides Temporin-Sha and [K3]SHa to LL-37 and Temporin-Tb against Herpes Simplex Virus Type 1 |
title_fullStr |
Comparison of Anti-Viral Activity of Frog Skin Anti-Microbial Peptides Temporin-Sha and [K3]SHa to LL-37 and Temporin-Tb against Herpes Simplex Virus Type 1 |
title_full_unstemmed |
Comparison of Anti-Viral Activity of Frog Skin Anti-Microbial Peptides Temporin-Sha and [K3]SHa to LL-37 and Temporin-Tb against Herpes Simplex Virus Type 1 |
title_sort |
comparison of anti-viral activity of frog skin anti-microbial peptides temporin-sha and [k3]sha to ll-37 and temporin-tb against herpes simplex virus type 1 |
publisher |
MDPI AG |
series |
Viruses |
issn |
1999-4915 |
publishDate |
2019-01-01 |
description |
Temporins are anti-microbial peptides synthesized in the skin of frogs of the Ranidae family. The few studies to date that have examined their anti-viral properties have shown that they have potential as anti-viral therapies. In this work, we evaluated the anti-herpes simplex virus type 1 (HSV-1) activity of the temporin-SHa (SHa) and its synthetic analog [K3]SHa. Human cathelicidin LL-37 and temporin-Tb (Tb), previously demonstrated to have anti-HSV-1 properties, were used as positive controls. We observed that SHa and [K3]SHa significantly inhibit HSV-1 replication in human primary keratinocytes when used at micromolar concentrations. This anti-viral activity was equivalent to that of Tb, but lower than that of LL-37. Transcriptomic analyses revealed that SHa did not act through the modulation of the cell innate immune response, but rather, displayed virucidal properties by reducing infectious titer of HSV-1 in suspension. In contrast, pre-incubation of the virus with LL-37 suggests that this peptide does not act directly on the viral particle at non-cytotoxic concentrations tested. The anti-HSV-1 activity of LL-37 appears to be due to the potentiation of cellular anti-viral defenses through the induction of interferon stimulated gene expression in infected primary keratinocytes. This study demonstrated that SHa and [K3]SHa, in addition to their previously reported antibacterial and antiparasitic activities, are direct-acting anti-HSV-1 peptides. Importantly, this study extends the little studied anti-viral attributes of frog temporins and offers perspectives for the development of new anti-HSV-1 therapies. |
topic |
temporin SHa [K3]SHa Tb LL-37 herpes simplex virus type 1 keratinocyte cytotoxicity anti-viral immunomodulation |
url |
http://www.mdpi.com/1999-4915/11/1/77 |
work_keys_str_mv |
AT maevaroy comparisonofantiviralactivityoffrogskinantimicrobialpeptidestemporinshaandk3shatoll37andtemporintbagainstherpessimplexvirustype1 AT lucielebeau comparisonofantiviralactivityoffrogskinantimicrobialpeptidestemporinshaandk3shatoll37andtemporintbagainstherpessimplexvirustype1 AT celinechessa comparisonofantiviralactivityoffrogskinantimicrobialpeptidestemporinshaandk3shatoll37andtemporintbagainstherpessimplexvirustype1 AT alexiadamour comparisonofantiviralactivityoffrogskinantimicrobialpeptidestemporinshaandk3shatoll37andtemporintbagainstherpessimplexvirustype1 AT aliladram comparisonofantiviralactivityoffrogskinantimicrobialpeptidestemporinshaandk3shatoll37andtemporintbagainstherpessimplexvirustype1 AT brunooury comparisonofantiviralactivityoffrogskinantimicrobialpeptidestemporinshaandk3shatoll37andtemporintbagainstherpessimplexvirustype1 AT davidboutolleau comparisonofantiviralactivityoffrogskinantimicrobialpeptidestemporinshaandk3shatoll37andtemporintbagainstherpessimplexvirustype1 AT charlesbodet comparisonofantiviralactivityoffrogskinantimicrobialpeptidestemporinshaandk3shatoll37andtemporintbagainstherpessimplexvirustype1 AT nicolasleveque comparisonofantiviralactivityoffrogskinantimicrobialpeptidestemporinshaandk3shatoll37andtemporintbagainstherpessimplexvirustype1 |
_version_ |
1725379251319865344 |