Resource partitioning between bacteria, fungi and protists in the detritusphere of an agricultural soil
The flow of plant-derived carbon in soil is a key component of global carbon cycling. Conceptual models of trophic carbon fluxes in soil have assumed separate bacterial and fungal energy channels in the detritusphere, controlled by both substrate complexity and recalcitrance. However, detailed under...
Main Authors: | , , , , , , , , , , , , |
---|---|
Format: | Article |
Language: | English |
Published: |
Frontiers Media S.A.
2016-09-01
|
Series: | Frontiers in Microbiology |
Subjects: | |
Online Access: | http://journal.frontiersin.org/Journal/10.3389/fmicb.2016.01524/full |
id |
doaj-93cd3f7336694a68abc9b589b5872551 |
---|---|
record_format |
Article |
spelling |
doaj-93cd3f7336694a68abc9b589b58725512020-11-24T21:04:40ZengFrontiers Media S.A.Frontiers in Microbiology1664-302X2016-09-01710.3389/fmicb.2016.01524217074Resource partitioning between bacteria, fungi and protists in the detritusphere of an agricultural soilSusanne Kramer0Dörte Dibbern1Julia Moll2Maike Huenninghaus3Robert Koller4Dirk Krueger5Sven Marhan6Tim Urich7Tesfaye Wubet8Michael Bonkowski9Francois Buscot10Tillmann Lueders11Ellen Kandeler12University of HohenheimHelmholtz Zentrum MuenchenUFZ – Helmholtz Centre for Environmental Research Leipzig-HalleUniversity of CologneUniversity of CologneUFZ – Helmholtz Centre for Environmental Research Leipzig-HalleUniversity of HohenheimErnst-Moritz-Arndt University of GreifswaldUFZ – Helmholtz Centre for Environmental Research Leipzig-HalleUniversity of CologneUFZ – Helmholtz Centre for Environmental Research Leipzig-HalleHelmholtz Zentrum MuenchenUniversity of HohenheimThe flow of plant-derived carbon in soil is a key component of global carbon cycling. Conceptual models of trophic carbon fluxes in soil have assumed separate bacterial and fungal energy channels in the detritusphere, controlled by both substrate complexity and recalcitrance. However, detailed understanding of the key populations involved and niche-partitioning between them is limited. Here, a microcosm experiment was performed to trace the flow of detritusphere C from substrate analogues (glucose, cellulose) and plant biomass amendments (maize leaves, roots) in an agricultural soil. Carbon flow was traced by rRNA stable isotope probing and amplicon sequencing across three microbial kingdoms. Distinct lineages within the Actinobacteria, Bacteroidetes, Gammaproteobacteria, Basidiomycota, Ascomycota as well as Peronosporomycetes were identified as important primary substrate consumers. A dynamic succession of primary consumers was observed especially in the cellulose treatments, but also in plant amendments over time. While intra-kingdom niche partitioning was clearly observed, distinct bacterial and fungal energy channels were not apparent. Furthermore, while the diversity of primary substrate consumers did not notably increase with substrate complexity, consumer succession and secondary trophic links to bacterivorous and fungivorous microbes resulted in increased food web complexity in the more recalcitrant substrates. This suggests that rather than substrate-defined energy channels, consumer succession as well as intra- and inter-kingdom cross-feeding should be considered as mechanisms supporting food web complexity in the detritusphere.http://journal.frontiersin.org/Journal/10.3389/fmicb.2016.01524/fullstable isotope probingAmplicon sequencingEnergy channelsPlant litter decompositionrRNA-SIP |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Susanne Kramer Dörte Dibbern Julia Moll Maike Huenninghaus Robert Koller Dirk Krueger Sven Marhan Tim Urich Tesfaye Wubet Michael Bonkowski Francois Buscot Tillmann Lueders Ellen Kandeler |
spellingShingle |
Susanne Kramer Dörte Dibbern Julia Moll Maike Huenninghaus Robert Koller Dirk Krueger Sven Marhan Tim Urich Tesfaye Wubet Michael Bonkowski Francois Buscot Tillmann Lueders Ellen Kandeler Resource partitioning between bacteria, fungi and protists in the detritusphere of an agricultural soil Frontiers in Microbiology stable isotope probing Amplicon sequencing Energy channels Plant litter decomposition rRNA-SIP |
author_facet |
Susanne Kramer Dörte Dibbern Julia Moll Maike Huenninghaus Robert Koller Dirk Krueger Sven Marhan Tim Urich Tesfaye Wubet Michael Bonkowski Francois Buscot Tillmann Lueders Ellen Kandeler |
author_sort |
Susanne Kramer |
title |
Resource partitioning between bacteria, fungi and protists in the detritusphere of an agricultural soil |
title_short |
Resource partitioning between bacteria, fungi and protists in the detritusphere of an agricultural soil |
title_full |
Resource partitioning between bacteria, fungi and protists in the detritusphere of an agricultural soil |
title_fullStr |
Resource partitioning between bacteria, fungi and protists in the detritusphere of an agricultural soil |
title_full_unstemmed |
Resource partitioning between bacteria, fungi and protists in the detritusphere of an agricultural soil |
title_sort |
resource partitioning between bacteria, fungi and protists in the detritusphere of an agricultural soil |
publisher |
Frontiers Media S.A. |
series |
Frontiers in Microbiology |
issn |
1664-302X |
publishDate |
2016-09-01 |
description |
The flow of plant-derived carbon in soil is a key component of global carbon cycling. Conceptual models of trophic carbon fluxes in soil have assumed separate bacterial and fungal energy channels in the detritusphere, controlled by both substrate complexity and recalcitrance. However, detailed understanding of the key populations involved and niche-partitioning between them is limited. Here, a microcosm experiment was performed to trace the flow of detritusphere C from substrate analogues (glucose, cellulose) and plant biomass amendments (maize leaves, roots) in an agricultural soil. Carbon flow was traced by rRNA stable isotope probing and amplicon sequencing across three microbial kingdoms. Distinct lineages within the Actinobacteria, Bacteroidetes, Gammaproteobacteria, Basidiomycota, Ascomycota as well as Peronosporomycetes were identified as important primary substrate consumers. A dynamic succession of primary consumers was observed especially in the cellulose treatments, but also in plant amendments over time. While intra-kingdom niche partitioning was clearly observed, distinct bacterial and fungal energy channels were not apparent. Furthermore, while the diversity of primary substrate consumers did not notably increase with substrate complexity, consumer succession and secondary trophic links to bacterivorous and fungivorous microbes resulted in increased food web complexity in the more recalcitrant substrates. This suggests that rather than substrate-defined energy channels, consumer succession as well as intra- and inter-kingdom cross-feeding should be considered as mechanisms supporting food web complexity in the detritusphere. |
topic |
stable isotope probing Amplicon sequencing Energy channels Plant litter decomposition rRNA-SIP |
url |
http://journal.frontiersin.org/Journal/10.3389/fmicb.2016.01524/full |
work_keys_str_mv |
AT susannekramer resourcepartitioningbetweenbacteriafungiandprotistsinthedetritusphereofanagriculturalsoil AT dortedibbern resourcepartitioningbetweenbacteriafungiandprotistsinthedetritusphereofanagriculturalsoil AT juliamoll resourcepartitioningbetweenbacteriafungiandprotistsinthedetritusphereofanagriculturalsoil AT maikehuenninghaus resourcepartitioningbetweenbacteriafungiandprotistsinthedetritusphereofanagriculturalsoil AT robertkoller resourcepartitioningbetweenbacteriafungiandprotistsinthedetritusphereofanagriculturalsoil AT dirkkrueger resourcepartitioningbetweenbacteriafungiandprotistsinthedetritusphereofanagriculturalsoil AT svenmarhan resourcepartitioningbetweenbacteriafungiandprotistsinthedetritusphereofanagriculturalsoil AT timurich resourcepartitioningbetweenbacteriafungiandprotistsinthedetritusphereofanagriculturalsoil AT tesfayewubet resourcepartitioningbetweenbacteriafungiandprotistsinthedetritusphereofanagriculturalsoil AT michaelbonkowski resourcepartitioningbetweenbacteriafungiandprotistsinthedetritusphereofanagriculturalsoil AT francoisbuscot resourcepartitioningbetweenbacteriafungiandprotistsinthedetritusphereofanagriculturalsoil AT tillmannlueders resourcepartitioningbetweenbacteriafungiandprotistsinthedetritusphereofanagriculturalsoil AT ellenkandeler resourcepartitioningbetweenbacteriafungiandprotistsinthedetritusphereofanagriculturalsoil |
_version_ |
1716770284941344768 |