Antibacterial Activity of Trypsin-Hydrolyzed Camel and Cow Whey and Their Fractions

• Main Authors: Ruixue Wang, Zhihao Han, Rimutu Ji, Yuchen Xiao, Rendalai Si, Fucheng Guo, Jing He, Le Hai, Liang Ming, Li Yi
• Format: Article
• Language: English
• Published: MDPI AG 2020-02-01
• Series: Animals
• Subjects: camel milk; cow milk; ultra-filtration; chromatography; amino acid; antimicrobial peptide
• Online Access: https://www.mdpi.com/2076-2615/10/2/337

Antibacterial peptides were isolated and purified from whey proteins of camel milk (CaW) and cow milk (CoW) and their antimicrobial activities were studied. The whey proteins were hydrolyzed using trypsin, and the degree of hydrolysis was identified by gel electrophoresis. The whey hydrolysate (WH) was purified using ultrafiltration and Dextran gel chromatography to obtain small peptides with antibacterial activity. The effect of the antimicrobial peptides on the morphology of bacterial strains was investigated using transmission electron microscopy. Their amino acid composition and antimicrobial activities were then determined. Polypeptides CaWH-III (&lt;3 kDa) and CoWH-III (&lt;3 kDa) had the strongest antibacterial activity. Both Fr.A2 (CaWH-Ⅲ&#8217;s fraction 2) and Fr.B1 (CoWH-Ⅲ&#8217;s fraction 1) had antibacterial effects toward <i>Escherichia coli</i> and <i>Staphylococcus aureus</i>, with minimum antimicrobial mass concentrations of 65 mg/mL and 130 mg/mL for Fr.A2, and 130 mg/mL and 130 mg/mL for Fr.B1, respectively. The highly active antimicrobial peptides had high amounts of alkaline amino acids (28.13% in camel milk Fr.A2 and 25.07% in the cow milk Fr.B1) and hydrophobic amino acids. (51.29% in camel milk Fr.A2 and 57.69% in the cow milk Fr.B1). This results showed that hydrolysis of CaW and CoW using trypsin produced a variety of effective antimicrobial peptides against selected pathogens, and the antibacterial activity of camel milk whey was slightly higher than that of cow milk whey.