Evidence for co-translational misincorporation of non-canonical amino acid hydroxyproline in recombinant antibodies produced in Chinese Hamster Ovary (CHO) cell lines.

Evidence for co-translational misincorporation of non-canonical amino acid hydroxyproline in recombinant antibodies produced in Chinese Hamster Ovary (CHO) cell lines.

With the advent of highly sensitive technologies such as tandem mass spectrometry and next-generation sequencing, recombinant antibodies are now routinely analyzed for the presence of low-level sequence variants including amino acid misincorporations. During mAb cell culture process development, we...

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Bibliographic Details
Main Authors: Shanta Boddapati, Jason Gilmore, Kyle Boone, John Bushey, Jonathan Ross, Brian Gfeller, William McFee, Romesh Rao, Greg Corrigan, Aaron Chen, Howard Clarke, John Valliere-Douglass, Swapnil Bhargava
Format: Article
Language:English
Published: Public Library of Science (PLoS) 2020-01-01
Series:PLoS ONE
Online Access:https://doi.org/10.1371/journal.pone.0241250

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