Establishment of a new cell line from lepidopteran epidermis and hormonal regulation on the genes.
When an insect molts, old cuticle on the outside of the integument is shed by apolysis and a new cuticle is formed under the old one. This process is completed by the epidermal cells which are controlled by 20-hydroxyecdysone (20E) and juvenile hormone. To understand the molecular mechanisms of inte...
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2008-01-01
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doaj-93ff986d91924430a5f06acd64c3596c2020-11-25T02:20:05ZengPublic Library of Science (PLoS)PLoS ONE1932-62032008-01-0139e312710.1371/journal.pone.0003127Establishment of a new cell line from lepidopteran epidermis and hormonal regulation on the genes.Hong-Lian ShaoWei-Wei ZhengPeng-Cheng LiuQian WangJin-Xing WangXiao-Fan ZhaoWhen an insect molts, old cuticle on the outside of the integument is shed by apolysis and a new cuticle is formed under the old one. This process is completed by the epidermal cells which are controlled by 20-hydroxyecdysone (20E) and juvenile hormone. To understand the molecular mechanisms of integument remolding and hormonal regulation on the gene expression, an epidermal cell line from the 5th instar larval integument of Helicoverpa armigera was established and named HaEpi. The cell line has been cultured continuously for 82 passages beginning on June 30, 2005 until now. Cell doubling time was 64 h. The chromosomes were granular and the chromosome mode was from 70 to 76. Collagenase I was used to detach the cells from the flask bottom. Non-self pathogen AcMNPV induced the cells to apoptosis. The cell line was proved to be an epidermal cell line based on its unique gene expression pattern. It responded to 20E and the non-steroidal ecdysone agonist RH-2485. Its gene expression could be knocked down using RNA interference. Various genes in the cell line were investigated based on their response to 20E. This new cell line represents a platform for investigating the 20E signaling transduction pathway, the immune response mechanism in lepidopteran epidermis and interactions of the genes.http://europepmc.org/articles/PMC2518862?pdf=render |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Hong-Lian Shao Wei-Wei Zheng Peng-Cheng Liu Qian Wang Jin-Xing Wang Xiao-Fan Zhao |
spellingShingle |
Hong-Lian Shao Wei-Wei Zheng Peng-Cheng Liu Qian Wang Jin-Xing Wang Xiao-Fan Zhao Establishment of a new cell line from lepidopteran epidermis and hormonal regulation on the genes. PLoS ONE |
author_facet |
Hong-Lian Shao Wei-Wei Zheng Peng-Cheng Liu Qian Wang Jin-Xing Wang Xiao-Fan Zhao |
author_sort |
Hong-Lian Shao |
title |
Establishment of a new cell line from lepidopteran epidermis and hormonal regulation on the genes. |
title_short |
Establishment of a new cell line from lepidopteran epidermis and hormonal regulation on the genes. |
title_full |
Establishment of a new cell line from lepidopteran epidermis and hormonal regulation on the genes. |
title_fullStr |
Establishment of a new cell line from lepidopteran epidermis and hormonal regulation on the genes. |
title_full_unstemmed |
Establishment of a new cell line from lepidopteran epidermis and hormonal regulation on the genes. |
title_sort |
establishment of a new cell line from lepidopteran epidermis and hormonal regulation on the genes. |
publisher |
Public Library of Science (PLoS) |
series |
PLoS ONE |
issn |
1932-6203 |
publishDate |
2008-01-01 |
description |
When an insect molts, old cuticle on the outside of the integument is shed by apolysis and a new cuticle is formed under the old one. This process is completed by the epidermal cells which are controlled by 20-hydroxyecdysone (20E) and juvenile hormone. To understand the molecular mechanisms of integument remolding and hormonal regulation on the gene expression, an epidermal cell line from the 5th instar larval integument of Helicoverpa armigera was established and named HaEpi. The cell line has been cultured continuously for 82 passages beginning on June 30, 2005 until now. Cell doubling time was 64 h. The chromosomes were granular and the chromosome mode was from 70 to 76. Collagenase I was used to detach the cells from the flask bottom. Non-self pathogen AcMNPV induced the cells to apoptosis. The cell line was proved to be an epidermal cell line based on its unique gene expression pattern. It responded to 20E and the non-steroidal ecdysone agonist RH-2485. Its gene expression could be knocked down using RNA interference. Various genes in the cell line were investigated based on their response to 20E. This new cell line represents a platform for investigating the 20E signaling transduction pathway, the immune response mechanism in lepidopteran epidermis and interactions of the genes. |
url |
http://europepmc.org/articles/PMC2518862?pdf=render |
work_keys_str_mv |
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