Mixture of Phlorotannin and Fucoidan from <i>Ecklonia cava</i> Prevents the Aβ-Induced Cognitive Decline with Mitochondrial and Cholinergic Activation
The anti-amnesic effect of a mixture (4:6 = phlorotannin:fucoidan from <i>Ecklonia cava</i>, P4F6) was evaluated on amyloid-beta peptide (Aβ)-induced cognitive deficit mice. The cognitive function was examined by Y-maze, passive avoidance, and Morris water maze tests, and the intake of t...
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MDPI AG
2021-07-01
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| Series: | Marine Drugs |
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| Online Access: | https://www.mdpi.com/1660-3397/19/8/434 |
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doaj-9404971622ea4e41865436ec7ef7919a2021-08-26T13:59:57ZengMDPI AGMarine Drugs1660-33972021-07-011943443410.3390/md19080434Mixture of Phlorotannin and Fucoidan from <i>Ecklonia cava</i> Prevents the Aβ-Induced Cognitive Decline with Mitochondrial and Cholinergic ActivationHye Ju Han0Seon Kyeong Park1Jin Yong Kang2Jong Min Kim3Seul Ki Yoo4Dae-Ok Kim5Gun-Hee Kim6Ho Jin Heo7Division of Applied Life Science (BK21), Institute of Agriculture and Life Science, Gyeongsang National University, Jinju 52828, KoreaDivision of Applied Life Science (BK21), Institute of Agriculture and Life Science, Gyeongsang National University, Jinju 52828, KoreaDivision of Applied Life Science (BK21), Institute of Agriculture and Life Science, Gyeongsang National University, Jinju 52828, KoreaDivision of Applied Life Science (BK21), Institute of Agriculture and Life Science, Gyeongsang National University, Jinju 52828, KoreaDivision of Applied Life Science (BK21), Institute of Agriculture and Life Science, Gyeongsang National University, Jinju 52828, KoreaDepartment of Food Science and Biotechnology, Kyung Hee University, Yongin 17104, KoreaDepartment of Food and Nutrition, Duksung Women’s University, Seoul 01369, KoreaDivision of Applied Life Science (BK21), Institute of Agriculture and Life Science, Gyeongsang National University, Jinju 52828, KoreaThe anti-amnesic effect of a mixture (4:6 = phlorotannin:fucoidan from <i>Ecklonia cava</i>, P4F6) was evaluated on amyloid-beta peptide (Aβ)-induced cognitive deficit mice. The cognitive function was examined by Y-maze, passive avoidance, and Morris water maze tests, and the intake of the mixture (P4F6) showed an ameliorating effect on Aβ-induced learning and memory impairment. After the behavioral tests, superoxide dismutase (SOD) activity and thiobarbituric acid-reactive substances (TBARS) contents were confirmed in brain tissue, and in the results, the mixture (P4F6) attenuated Aβ-induced oxidative stress. In addition, mitochondrial activity was evaluated by mitochondrial reactive oxygen species (ROS) content, mitochondrial membrane potential (MMP), adenosine triphosphate (ATP) content, and mitochondria-mediated apoptotic signaling pathway, and the mixture (P4F6) enhanced mitochondrial function. Furthermore, the mixture (P4F6) effectively regulated tau hyperphosphorylation by regulating the protein kinase B (Akt) pathway, and promoted brain-derived neurotrophic factor (BDNF) in brain tissue. Moreover, in the cholinergic system, the mixture (P4F6) ameliorated acetylcholine (ACh) content by regulating acetylcholinesterase (AChE) activity and choline acetyltransferase (ChAT) expression in brain tissue. Based on these results, we suggest that this mixture of phlorotannin and fucoidan (P4F6) might be a substance for improving cognitive function by effectively regulating cognition-related molecules.https://www.mdpi.com/1660-3397/19/8/434<i>Ecklonia cava</i>phlorotanninfucoidanamyloid-betacognitionmitochondrial function |
| collection |
DOAJ |
| language |
English |
| format |
Article |
| sources |
DOAJ |
| author |
Hye Ju Han Seon Kyeong Park Jin Yong Kang Jong Min Kim Seul Ki Yoo Dae-Ok Kim Gun-Hee Kim Ho Jin Heo |
| spellingShingle |
Hye Ju Han Seon Kyeong Park Jin Yong Kang Jong Min Kim Seul Ki Yoo Dae-Ok Kim Gun-Hee Kim Ho Jin Heo Mixture of Phlorotannin and Fucoidan from <i>Ecklonia cava</i> Prevents the Aβ-Induced Cognitive Decline with Mitochondrial and Cholinergic Activation Marine Drugs <i>Ecklonia cava</i> phlorotannin fucoidan amyloid-beta cognition mitochondrial function |
| author_facet |
Hye Ju Han Seon Kyeong Park Jin Yong Kang Jong Min Kim Seul Ki Yoo Dae-Ok Kim Gun-Hee Kim Ho Jin Heo |
| author_sort |
Hye Ju Han |
| title |
Mixture of Phlorotannin and Fucoidan from <i>Ecklonia cava</i> Prevents the Aβ-Induced Cognitive Decline with Mitochondrial and Cholinergic Activation |
| title_short |
Mixture of Phlorotannin and Fucoidan from <i>Ecklonia cava</i> Prevents the Aβ-Induced Cognitive Decline with Mitochondrial and Cholinergic Activation |
| title_full |
Mixture of Phlorotannin and Fucoidan from <i>Ecklonia cava</i> Prevents the Aβ-Induced Cognitive Decline with Mitochondrial and Cholinergic Activation |
| title_fullStr |
Mixture of Phlorotannin and Fucoidan from <i>Ecklonia cava</i> Prevents the Aβ-Induced Cognitive Decline with Mitochondrial and Cholinergic Activation |
| title_full_unstemmed |
Mixture of Phlorotannin and Fucoidan from <i>Ecklonia cava</i> Prevents the Aβ-Induced Cognitive Decline with Mitochondrial and Cholinergic Activation |
| title_sort |
mixture of phlorotannin and fucoidan from <i>ecklonia cava</i> prevents the aβ-induced cognitive decline with mitochondrial and cholinergic activation |
| publisher |
MDPI AG |
| series |
Marine Drugs |
| issn |
1660-3397 |
| publishDate |
2021-07-01 |
| description |
The anti-amnesic effect of a mixture (4:6 = phlorotannin:fucoidan from <i>Ecklonia cava</i>, P4F6) was evaluated on amyloid-beta peptide (Aβ)-induced cognitive deficit mice. The cognitive function was examined by Y-maze, passive avoidance, and Morris water maze tests, and the intake of the mixture (P4F6) showed an ameliorating effect on Aβ-induced learning and memory impairment. After the behavioral tests, superoxide dismutase (SOD) activity and thiobarbituric acid-reactive substances (TBARS) contents were confirmed in brain tissue, and in the results, the mixture (P4F6) attenuated Aβ-induced oxidative stress. In addition, mitochondrial activity was evaluated by mitochondrial reactive oxygen species (ROS) content, mitochondrial membrane potential (MMP), adenosine triphosphate (ATP) content, and mitochondria-mediated apoptotic signaling pathway, and the mixture (P4F6) enhanced mitochondrial function. Furthermore, the mixture (P4F6) effectively regulated tau hyperphosphorylation by regulating the protein kinase B (Akt) pathway, and promoted brain-derived neurotrophic factor (BDNF) in brain tissue. Moreover, in the cholinergic system, the mixture (P4F6) ameliorated acetylcholine (ACh) content by regulating acetylcholinesterase (AChE) activity and choline acetyltransferase (ChAT) expression in brain tissue. Based on these results, we suggest that this mixture of phlorotannin and fucoidan (P4F6) might be a substance for improving cognitive function by effectively regulating cognition-related molecules. |
| topic |
<i>Ecklonia cava</i> phlorotannin fucoidan amyloid-beta cognition mitochondrial function |
| url |
https://www.mdpi.com/1660-3397/19/8/434 |
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